CAJ | 학술논문

目的探讨特发性肺纤维化(IPF)患者血清基质金属蛋白酶(MMPs)水平及其临床意义. 方法 IPF患者(IPF组)39例和健康体检者(对照组)36例,采集外周静脉血5 ml,采用酶联免疫吸附法检测血清MMP 1、MMP-2、MMP-9水平,同时对IPF患者进行用力肺活量(FVC)和一氧化碳弥散量(DLCO)测定,比较两组血清MMP-1、MMP-2和MMP-9水平,以及IPF组患者血清MMPs水平与肺功能指标的相关性. 结果 IPF组患者血清MMP-1、MMP-2和MMP-9中位数(范围)分别为906.30(613.50～1139.23) μg/L、9.13(5.95～11.92)μg/L和14.56(8.24～18.96)μg/L,均明显高于对照组808.38(596.59～1249.36)μg/L、7.72(5.28～10.35)μg/L和11.38(8.07～17.14)μg/L,两组血清MMP-1、MMP-2和MMP-9水平的差异有统计学意义(Z=2.397、-3.564、-4.152,P=0.017、0.000、0.000).IPF组患者血清MMP-2水平与DLCO占预计值百分比呈负相关(r=-0.53,P=0.011),MMP-9水平与FVC占预计值百分比、DLCO占预计值百分比均呈负相关(r=-0.49、-0.58,P=0.020、0.005). 结论联合检测血清MMP-1、MMP-2和MMP-9水平有助于临床诊断IPF,MMP 2和MMP-9可能成为预测IPF预后的生物标志物.
목적 탐토특발성폐섬유화(IPF)환자혈청기질금속단백매(MMPs)수평급기림상의의. 방법 IPF환자(IPF조)39례화건강체검자(대조조)36례,채집외주정맥혈5 ml,채용매련면역흡부법검측혈청MMP 1、MMP-2、MMP-9수평,동시대IPF환자진행용력폐활량(FVC)화일양화탄미산량(DLCO)측정,비교량조혈청MMP-1、MMP-2화MMP-9수평,이급IPF조환자혈청MMPs수평여폐공능지표적상관성. 결과 IPF조환자혈청MMP-1、MMP-2화MMP-9중위수(범위)분별위906.30(613.50～1139.23) μg/L、9.13(5.95～11.92)μg/L화14.56(8.24～18.96)μg/L,균명현고우대조조808.38(596.59～1249.36)μg/L、7.72(5.28～10.35)μg/L화11.38(8.07～17.14)μg/L,량조혈청MMP-1、MMP-2화MMP-9수평적차이유통계학의의(Z=2.397、-3.564、-4.152,P=0.017、0.000、0.000).IPF조환자혈청MMP-2수평여DLCO점예계치백분비정부상관(r=-0.53,P=0.011),MMP-9수평여FVC점예계치백분비、DLCO점예계치백분비균정부상관(r=-0.49、-0.58,P=0.020、0.005). 결론 연합검측혈청MMP-1、MMP-2화MMP-9수평유조우림상진단IPF,MMP 2화MMP-9가능성위예측IPF예후적생물표지물.
Objective To investigate serum levels of matrix metalloproteinase (MMP) 1,-2 and -9 in patients with idiopathic pulmonary fibrosis (IPF),and to assess their clinical value.Methods 39 clinically confirmed IPF cases and 36 healthy controls were recruited.A sample of 5 ml peripheral blood was collected from each individual and serum levels of MMP-1,-2 and 9 were detected by the enzyme-linked immunosorbent assay.Forced vital capacity (FVC) and diffusing capacity of carbon monoxide (DLCO) were measured in IPF patients.Serum levels of MMP 1,2 and-9 were compared between the groups.The correlations between serum levels of MMPs and lung function indicators were analyzed.Results The median serum levels of MMP 1,MMP-2 and MMP-9 were higher in the IPF group than in the control group [906.30 (613.50-1139.23) μg/L vs.808.38 (596.59 1249.36) μg/L,9.13 (5.95-11.92) μg/L vs.7.72 (5.28 10.35) μg/L,14.56 (8.24-18.96) μg/L vs.11.38 (8.07-17.14) μg/L,Z=-2.397,-3.564,-4.152,respectively,P=0.017,0.000,0.000].In patients with IPF,the serum level of MMP-2 was negatively correlated with DLCO％ (r=-0.53,P=0.011) and the serum level of MMP-9 had a negative correlation with FVC％ (r=-0.49,P=0.020) and DLCO％ (r=0.58,P=0.005).Conclusions Taken together,serum MMP-1,MMP-2 and MMP-9 levels may help in the clinical diagnosis of IPF.MMP 2 and MMP-9 may be important biomarkers to predict the prognosis of IPF.