CAJ | 학술논문

目的探讨高迁移率族蛋白-1(HMGB1)在尿源性脓毒血症家兔肾组织中的表达水平及意义.方法健康雄性家兔随机分为假手术组(Sham组)和尿源性脓毒血症组(US组).采用肾盂内高压法制备家兔尿源性脓毒血症模型.分别于术后12、24、36 h检测血白细胞计数、肾功能、早期炎性因子肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6),以及肾组织中HMGB1 mRNA表达.结果与同时间点Sham组比较,US组血白细胞计数[36 h(×109/L):17.22 ±0.32比9.54±0.24]、尿素氮(BUN)[36 h (mmol/L):11.71±0.35比7.14 ±0.12]、肌酐(Cr) [36 h(μmol/L):136.10 ±7.39比82.74 ±2.18]水平均明显升高(P＜0.05);US组血清TNF-α和IL-6水平先升高后降低,12 h[TNF-α (ng/L):124.6 ±4.42比18.18±1.26;IL-6 (ng/L):120.5±6.99比51.65 ±2.26]和24 h[TNF-α (ng/L):96.94 ±7.95比19.04 ±0.89;IL-6 (ng/L):83.22±4.23比53.95±2.26]与Sham组比较差异有统计学意义(P＜0.05);US组肾组织中HMGB1 mRNA表达逐渐升高,各时间点与Sham组比较差异有统计学意义(P＜0.05).结论尿源性脓毒症致急性肾损伤病程中,家兔肾组织HMGB1表达逐渐升高.
목적 탐토고천이솔족단백-1(HMGB1)재뇨원성농독혈증가토신조직중적표체수평급의의.방법 건강웅성가토수궤분위가수술조(Sham조)화뇨원성농독혈증조(US조).채용신우내고압법제비가토뇨원성농독혈증모형.분별우술후12、24、36 h검측혈백세포계수、신공능、조기염성인자종류배사인자-α(TNF-α)화백세포개소-6(IL-6),이급신조직중HMGB1 mRNA표체.결과 여동시간점Sham조비교,US조혈백세포계수[36 h(×109/L):17.22 ±0.32비9.54±0.24]、뇨소담(BUN)[36 h (mmol/L):11.71±0.35비7.14 ±0.12]、기항(Cr) [36 h(μmol/L):136.10 ±7.39비82.74 ±2.18]수평균명현승고(P＜0.05);US조혈청TNF-α화IL-6수평선승고후강저,12 h[TNF-α (ng/L):124.6 ±4.42비18.18±1.26;IL-6 (ng/L):120.5±6.99비51.65 ±2.26]화24 h[TNF-α (ng/L):96.94 ±7.95비19.04 ±0.89;IL-6 (ng/L):83.22±4.23비53.95±2.26]여Sham조비교차이유통계학의의(P＜0.05);US조신조직중HMGB1 mRNA표체축점승고,각시간점여Sham조비교차이유통계학의의(P＜0.05).결론 뇨원성농독증치급성신손상병정중,가토신조직HMGB1표체축점승고.
Objective To investigate the expression and significance of high mobility group box 1 (HMGB1) in the renal tissues of rabbits with urosepsis.Methods Male rabbits were randomly divided into two group:sham group and urosepsis group.The rabbit urosepsis model was established by artificial high pressure in the infected pelvis.The blood white blood cells (WBC) count,serum levels of blood urea nitrogen (BUN),creatinine (Cr),tumor necrosis factor-α (TNF-α),interleukin-6 (IL-6),and the expression of HMGB1 mRNA in the renal tissues of the two groups were observed at 12th,24th and 36th h after operation.Results As compared with sham group,the WBC count [36 h (× 109/L):17.22 ± 0.32 vs.9.54 ±0.24],serum levels of BUN [36 h (mmol/L):11.71 ±0.35 vs.7.14 ±0.12] and Cr [36 h (μmol/L):136.10 ± 7.39 vs.82.74 ± 2.18] in urosepsis group were significantly increased at every time point (P ＜ 0.05).The serun levels of TNF-α and IL-6 were first increased and then decreased in urosepsis group as compared with sham group at 12th h [TNF-α (ng/L):124.6 ± 4.42 vs.18.18 ± 1.26;IL-6 (ng/L):120.5 ±6.99 vs.51.65 ±2.26] and 24 h [TNF-α (ng/L):96.94 ±7.95 vs.19.04 ±0.89;IL-6 (ng/L):83.22 ±4.23 vs.53.95 ±2.26].The expression of HMGB1 mRNA was gradually increased from 12th to 36th h in urosepsis group as compared with sham group at the same time point (P ＜ 0.05).Conclusion In the progression of acute kidney injury induced by urosepsis,the expression of HMGB1 mRNA was gradually increased in renal tissues.