CAJ | 학술논문

目的观察吴茱萸碱联合顺铂对前列腺癌PC-3细胞增殖和细胞间缝隙连接通讯(GJIC)的影响.方法体外培养PC-3细胞,分为顺铂组(5、10、15、20 μmol/L)和顺铂(5、10、15、20 μmol/L)+吴茱萸碱组(0.4μmol/L)作用PC-3细胞.细胞计数试剂盒(CCK-8)法检测细胞增殖率变化;流式细胞术检测细胞凋亡变化;Western blot分别检测间隙连接蛋白43(Cx43)蛋白表达;荧光光漂白恢复技术检测细胞缝隙连接(GJ)功能改变.结果顺铂组荧光恢复率分别为(41.93±1.30)％、(36.32±2.33)％、(30.81±1.43)％、(22.54±1.70)％,顺铂+吴茱萸碱组分别为(69.86±2.16)％、(50.03±5.00)％、(45.38 ±2.59)％、(34.01±4.01)％.与顺铂组比较,顺铂联合吴茱萸碱组荧光恢复率、细胞凋亡水平、Cx43蛋白表达水平均明显升高(P＜0.05).结论吴茱萸碱可改善前列腺癌PC-3细胞株对顺铂耐药性,增强顺铂对前列腺癌PC-3细胞杀伤作用.
목적 관찰오수유감연합순박대전렬선암PC-3세포증식화세포간봉극련접통신(GJIC)적영향.방법 체외배양PC-3세포,분위순박조(5、10、15、20 μmol/L)화순박(5、10、15、20 μmol/L)+오수유감조(0.4μmol/L)작용PC-3세포.세포계수시제합(CCK-8)법검측세포증식솔변화;류식세포술검측세포조망변화;Western blot분별검측간극련접단백43(Cx43)단백표체;형광광표백회복기술검측세포봉극련접(GJ)공능개변.결과 순박조형광회복솔분별위(41.93±1.30)％、(36.32±2.33)％、(30.81±1.43)％、(22.54±1.70)％,순박+오수유감조분별위(69.86±2.16)％、(50.03±5.00)％、(45.38 ±2.59)％、(34.01±4.01)％.여순박조비교,순박연합오수유감조형광회복솔、세포조망수평、Cx43단백표체수평균명현승고(P＜0.05).결론 오수유감가개선전렬선암PC-3세포주대순박내약성,증강순박대전렬선암PC-3세포살상작용.
Objective To evaluate the effect of the combination of cisplatin and evodiamine on the growth of PC-3 cells and gap junction intercellular communication (GJIC).Methods Human prostate cancer PC-3 cells in logarithmic growth phase were cultured in vitro and then treated with various concentrations of cisplatin (5,10,15,20 μmol/L) or evodiamine (0.4 μmol/L) combined with various concentrations of cisplatin (5,10,15,20 μmol/L).The effects of the proliferation was investigated by cell counting kit-8 (CCK-8) assay.Apoptosis rate,and cell cycle distribution were examined by Annexin V-fluoresceine isothiocyanate (FITC)/propidiun iodide (PI) double staining flow cytometry.The expression of connexin43 (Cx43) protein was detected by Western blotting.The changes in the GJIC function were observed by FRAP.Results Fluorescence recovery rate was (41.93 ± 1.30) ％,(36.32 ± 2.33) ％,(30.81 ± 1.43)％,and (22.54 ±1.70)％ in cisplatin groups,and (69.86 ±2.16)％,(50.03 ± 5.00)％,(45.38 ±2.59)％,and (34.01 ±4.01)％ in Csplatin + evodiamine groups with statistically significant differences within groups respectively (P ＜ 0.05 for all).Fluorescence recovery rate,apoptosis rate,and the expression of Cx43 protein in cisplatin + evodiamine groups were significantly increased as compared with cisplatin groups (P ＜ 0.05 for all).Conclusion Evodiamine could recover the gap junction intercellular communication through increasing the expression of Cx43 protein and enhancing the cytotoxicity of cisplatin to prostate cancer PC-3 cells.