中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
6期
1351-1354
,共4页
胶质瘤%阿霉素%N-琥珀酰壳聚糖%缓释微粒
膠質瘤%阿黴素%N-琥珀酰殼聚糖%緩釋微粒
효질류%아매소%N-호박선각취당%완석미립
Glioma%Adriamycin%N-succinyl chitosan%Sustained release particles
目的 观察自制阿霉素-N-琥珀酰壳聚糖纳米缓释微粒在体内、外释药效果及对小鼠C6胶质瘤动物模型的治疗作用.方法 合成阿霉素-N-琥珀酰壳聚糖纳米缓释微粒,检测其在人工脑脊液及20只正常SD大鼠脑内药物释放的情况.检测阿霉素-N-琥珀酰壳聚糖纳米缓释微粒对20只脑内荷瘤SD小鼠生存期的影响以及对20只SD小鼠皮下接种肿瘤生长的抑制作用.结果 阿霉素-N-琥珀酰壳聚糖纳米缓释微粒在人工脑脊液和兔脑内可持续释放达30 d以上;局部控释化疗可明显延长脑内荷瘤小鼠的生存期,实验组中位生存时间为35 d.局部阿霉素控释化疗对于皮下肿瘤有明显抑制效应,与注射的对照组比较,实验组小鼠皮下瘤体生长减慢甚至停止生长,两组之间肿瘤体积差异有统计学意义(x2=7.68,P＜0.05),肿瘤细胞凋亡指数为:对照组为6.4％、实验组为59.3％,差异有统计学意义(x2=23.59,P＜0.05).结论 阿霉素-N-琥珀酰壳聚糖纳米缓释微粒的控释化疗在避免系统化疗全身毒性的同时,增加肿瘤局部药物浓度,可有效抑制肿瘤生长.
目的 觀察自製阿黴素-N-琥珀酰殼聚糖納米緩釋微粒在體內、外釋藥效果及對小鼠C6膠質瘤動物模型的治療作用.方法 閤成阿黴素-N-琥珀酰殼聚糖納米緩釋微粒,檢測其在人工腦脊液及20隻正常SD大鼠腦內藥物釋放的情況.檢測阿黴素-N-琥珀酰殼聚糖納米緩釋微粒對20隻腦內荷瘤SD小鼠生存期的影響以及對20隻SD小鼠皮下接種腫瘤生長的抑製作用.結果 阿黴素-N-琥珀酰殼聚糖納米緩釋微粒在人工腦脊液和兔腦內可持續釋放達30 d以上;跼部控釋化療可明顯延長腦內荷瘤小鼠的生存期,實驗組中位生存時間為35 d.跼部阿黴素控釋化療對于皮下腫瘤有明顯抑製效應,與註射的對照組比較,實驗組小鼠皮下瘤體生長減慢甚至停止生長,兩組之間腫瘤體積差異有統計學意義(x2=7.68,P＜0.05),腫瘤細胞凋亡指數為:對照組為6.4％、實驗組為59.3％,差異有統計學意義(x2=23.59,P＜0.05).結論 阿黴素-N-琥珀酰殼聚糖納米緩釋微粒的控釋化療在避免繫統化療全身毒性的同時,增加腫瘤跼部藥物濃度,可有效抑製腫瘤生長.
목적 관찰자제아매소-N-호박선각취당납미완석미립재체내、외석약효과급대소서C6효질류동물모형적치료작용.방법 합성아매소-N-호박선각취당납미완석미립,검측기재인공뇌척액급20지정상SD대서뇌내약물석방적정황.검측아매소-N-호박선각취당납미완석미립대20지뇌내하류SD소서생존기적영향이급대20지SD소서피하접충종류생장적억제작용.결과 아매소-N-호박선각취당납미완석미립재인공뇌척액화토뇌내가지속석방체30 d이상;국부공석화료가명현연장뇌내하류소서적생존기,실험조중위생존시간위35 d.국부아매소공석화료대우피하종류유명현억제효응,여주사적대조조비교,실험조소서피하류체생장감만심지정지생장,량조지간종류체적차이유통계학의의(x2=7.68,P＜0.05),종류세포조망지수위:대조조위6.4％、실험조위59.3％,차이유통계학의의(x2=23.59,P＜0.05).결론 아매소-N-호박선각취당납미완석미립적공석화료재피면계통화료전신독성적동시,증가종류국부약물농도,가유효억제종류생장.
Objective To observe the in vitro and in vivoreleasing effects of adriamycin-N-succinyl chitosan (SCS) sustained release nanometer particles following implantation,and evaluate the local chemotherapeutic effect on rats bearing C6 gliomas.Methods The matrix with adriamycin was prepared.Gelatin and agar were used as major materials.The releasing effects of adriamycin-SCS sustained release nanometer particles were determined in artificial cerebrospinal fluid or in 20 normal rats brains by the method of fluorometry.The median survival time (MST) was measured in 20 SD rats bearing intracranial C6 gliomas,and the inhibitory effect of regional administration of adriamycin-SCS on growth of subcutaneous tumors was evaluated in 20 rats.Results Constant-release of adriamycin-SCS lasted over 30 days in artificial cerebro-spinal fluid or normal rabbit brains.Local controlled release of adriamycin could prolong the survival of rats bearing C6 gliomas with the MST being 35 days.Local controlled release of adriamycin could significant inhibit the growth of the subcutaneous tumors.As compared with control group,the growth of the subcutaneous tumors was slowed down or ceased in experimental group.There was statistically significant difference in tumor volume between the two groups (x2 =7.68,P ＜ 0.05).There was significant difference in apoptosis index between control group (6.4％) and experimental group (59.3％,x2 =23.59,P ＜ 0.05).Conclusion With high regional concentration and avoidance of toxicity of systemic chemotherapy,control released chemotherapy of adriamycin-SCS sustained nanometer particles remarkably inhibited the growth of C6 gliomas.
