CAJ | 학술논문

目的观察人类表皮生长因子受体-2(Her-2)/neu基因对脑膜瘤细胞增殖能力的影响.方法收集脑膜瘤Her-2阳性患者术后新鲜标本5例,原代培养细胞,用脂质体法干扰细胞Her-2基因后,分别采用反转录-聚合酶链反应(RT-PCR)、Western blot法检测Her-2 mRNA和蛋白的表达;噻唑蓝(MTT)法、流式细胞术检测细胞增殖活性及周期的变化.结果转染后72 h,干扰组与阴性对照组比较,Her-2 mRNA和蛋白表达水平分别下降56.3％和52.7％,差异有统计学意义(P＜0.05);干扰组转染后72 h的抑制作用最强,细胞增殖抑制率为50.08％,细胞周期中G0/G1期细胞比例上升18.11％,S期比例下降39.34％,差异有统计学意义(P＜0.05).结论 Her-2/neu小分子干扰RNA可抑制脑膜瘤细胞的增殖活性.
목적 관찰인류표피생장인자수체-2(Her-2)/neu기인대뇌막류세포증식능력적영향.방법 수집뇌막류Her-2양성환자술후신선표본5례,원대배양세포,용지질체법간우세포Her-2기인후,분별채용반전록-취합매련반응(RT-PCR)、Western blot법검측Her-2 mRNA화단백적표체;새서람(MTT)법、류식세포술검측세포증식활성급주기적변화.결과 전염후72 h,간우조여음성대조조비교,Her-2 mRNA화단백표체수평분별하강56.3％화52.7％,차이유통계학의의(P＜0.05);간우조전염후72 h적억제작용최강,세포증식억제솔위50.08％,세포주기중G0/G1기세포비례상승18.11％,S기비례하강39.34％,차이유통계학의의(P＜0.05).결론 Her-2/neu소분자간우RNA가억제뇌막류세포적증식활성.
Objective To investigate the effects of human epidermal growth factor receptor-2 (Her-2)/neu gene on cell proliferative activity in human meningiomas.Methods The surgicall resected fresh samples from 5 cases of human meningiomas with Her-2 overexpression were selected,and cells were primarily cultured.The expression of Her-2 mRNA and protein was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting respectively.Methyl thiazol tetrazolium (MTT) assay was employed to examined the cell viability.The cell cycle distribution was measured by flow cytometry.Results At 72nd h after transfection,as compared with the silencing group and the negative control group,the Her-2 mRNA and protein expression was reduced by 56.3％ and 52.7％ respectively (P ＜ 0.05).At 72nd h after transfection,the proliferation inhibition rate was 50.08％.The proportion of cells in G0/G1 phase was increased by 18.11％,and that in S phase decreased by 39.34％ (P ＜0.05).Conclusion Her-2 small interfering RNA (siRNA)-mediated gene may inhibit the proliferative ablity of human meningiomas.