CAJ | 학술논문

目的探讨自体红骨髓移植在治疗胫骨骨不连大鼠模型中的作用.方法选取SD大鼠80只,截断其胫骨中段,灼烧骨折断端(包括骨膜),用克氏针钻入骨髓腔,制作胫骨骨不连大鼠模型,造模成功后选取其中40只大鼠行自体红骨髓移植,在大鼠模型髂前上棘或髂后上棘穿刺2～3个点,抽取红骨髓,即刻在直视下将骨髓缓慢注入骨不连部位.另40只大鼠作为对照组,未行红骨髓移植.术后大鼠模型全身给予抗生素,清洁换药,骨折肢体制动等.3个月后处死大鼠,经大体标本、病理组织学及放射学检查确定两组大鼠模型胫骨骨不连愈合情况.结果 X线检查显示,行自体红骨髓移植组大鼠术后4周胫骨骨折骨不连区域可见不规则的新生骨阴影.术后8周,骨阴影增多,遍布骨不连区,密度增高.术后12周,自体骨髓移植鼠模型胫骨骨不连消失,出现髓腔.对照组大鼠模型胫骨骨不连未发生骨性愈合,骨缺损均存在.病理检查示:术后12周,移植组所有自体骨髓移植组胫骨骨不连已形成骨性愈合,中央的骨小梁明显减少,髓腔融合,但不规则,而对照组的大鼠模型,胫骨皮质变薄,甚至穿孔,无1例形成骨性愈合,骨缺损区为纤维组织.两组差异有统计学意义(P＜0.01).结论自体红骨髓移植治疗胫骨骨不连大鼠模型方法简单,效果较好,可有效促进胫骨骨不连的愈合,恢复大鼠行走功能.
목적 탐토자체홍골수이식재치료경골골불련대서모형중적작용.방법 선취SD대서80지,절단기경골중단,작소골절단단(포괄골막),용극씨침찬입골수강,제작경골골불련대서모형,조모성공후선취기중40지대서행자체홍골수이식,재대서모형가전상극혹가후상극천자2～3개점,추취홍골수,즉각재직시하장골수완만주입골불련부위.령40지대서작위대조조,미행홍골수이식.술후대서모형전신급여항생소,청길환약,골절지체제동등.3개월후처사대서,경대체표본、병리조직학급방사학검사학정량조대서모형경골골불련유합정황.결과 X선검사현시,행자체홍골수이식조대서술후4주경골골절골불련구역가견불규칙적신생골음영.술후8주,골음영증다,편포골불련구,밀도증고.술후12주,자체골수이식서모형경골골불련소실,출현수강.대조조대서모형경골골불련미발생골성유합,골결손균존재.병리검사시:술후12주,이식조소유자체골수이식조경골골불련이형성골성유합,중앙적골소량명현감소,수강융합,단불규칙,이대조조적대서모형,경골피질변박,심지천공,무1례형성골성유합,골결손구위섬유조직.량조차이유통계학의의(P＜0.01).결론 자체홍골수이식치료경골골불련대서모형방법간단,효과교호,가유효촉진경골골불련적유합,회복대서행주공능.
Objective To investigate the effect of autologous redbone marrow transplantation for rat tibial nonunion model.Methods Select 80 SD rats,to cut off its tibia andburning the broken end of the fracture (including periosteal),using Kirschner wiredrilled into the bone marrow cavity,making the nonunion of the tibiaof rats model,after the success of modeling which selected from 40 rats underwent autologous red bone marrowtransplantationin the rat models ofanterior superior iliac spineor posterior superior iliac spine puncture 2-3 points,the extractionof red bone marrow,bone marrow immediatelyunder direct vision is slowly injected intothe nonunion site.The other 40 rats as the control group,without red bone marrowtransplantation.The rat model ofpostoperative systemicadministration of antibiotics,cleandressing,bone fracture limbs brakingetc.3 months after the rats were sacrificedbygross specimen,histopathologicaland radiological examination confirmed thetibia of tworats in the model groupwithout healing.Results X-ray inspection showed that:autologous red bone marrow transplantation rats,4 weeks after operation,new bone shadow region showed irregular bone nonunion of tibial fracture.8 weeks after operation,bone nonunion shadow increased throughout the region,increased density.12 weeks after the transplantation,autologous bone marrow transplantation rat model of tibial nonunion disappear,medullary cavity.The control rats in the model group,the tibial nonunion did not occur in the bone healing,bone defect exists.Pathological examination showed:12 weeks after transplantation,transplantation of autologous bone marrow transplantation group,all groups of nonunion of tibia has formed the bone healing,bone little Liang Mingxian central reduction,medullary cavity fusion,but not very rules,while the control grouprats model,tibialcortical thinning,even perforation.1 cases of no formation of bone healing,bone defect areato fibrous tissue.There was a statistically significantdifference between the two groups (P ＜0.01).Conclusion Autologous red bone marrowtransplantation for the treatment of tibialnonunionmethodsratmodel is simple,the effect is better,can effectively promotethe healing oftibialnonunion,walkingfunction recovery of rat model.