CAJ | 학술논문

目的检测溃疡性结肠炎(UC)患者外周血中CC趋化因子配体20(CCL20)表达水平,分析CCL20表达水平与UC严重程度的关系.方法入组52例UC患者(根据病情分为轻、中、重度)及30例健康体检者(对照组),分别收集外周血,Trizol法提取外周血中淋巴细胞总RNA,用实时定量聚合酶链反应(Real-time PCR)检测CCL20 mRNA表达水平;酶联免疫吸附试验(ELISA)检测外周血中CCL20蛋白质表达水平,独立样本t检验比较两组外周血中CCL20表达水平,方差分析比较轻、中、重度UC患者CCL20表达水平的差异.结果 UC组患者外周血中CCL20蛋白表达为(2 040.03 ± 730.07) ng/L,对照组外周血中CCL20蛋白表达为(550.01 ±70.71) ng/L,两组差异有统计学意义(P＜0.05);轻、中、重度UC患者外周血中CCL20蛋白表达水平分别为(1 233.33±251.66)、(2300.01 ±300.00)、(3 034.56±152.75) ng/L,其差异有统计学意义(P＜0.05).结论 UC患者外周血中CCL20表达增高,CCL20可能参与UC的发病.
목적 검측궤양성결장염(UC)환자외주혈중CC추화인자배체20(CCL20)표체수평,분석CCL20표체수평여UC엄중정도적관계.방법 입조52례UC환자(근거병정분위경、중、중도)급30례건강체검자(대조조),분별수집외주혈,Trizol법제취외주혈중림파세포총RNA,용실시정량취합매련반응(Real-time PCR)검측CCL20 mRNA표체수평;매련면역흡부시험(ELISA)검측외주혈중CCL20단백질표체수평,독립양본t검험비교량조외주혈중CCL20표체수평,방차분석비교경、중、중도UC환자CCL20표체수평적차이.결과 UC조환자외주혈중CCL20단백표체위(2 040.03 ± 730.07) ng/L,대조조외주혈중CCL20단백표체위(550.01 ±70.71) ng/L,량조차이유통계학의의(P＜0.05);경、중、중도UC환자외주혈중CCL20단백표체수평분별위(1 233.33±251.66)、(2300.01 ±300.00)、(3 034.56±152.75) ng/L,기차이유통계학의의(P＜0.05).결론 UC환자외주혈중CCL20표체증고,CCL20가능삼여UC적발병.
Objective To explore the expression of chemokine (C-C motif) ligand 20 (CCL20) in peripheral blood of patients with ulcerative colitis (UC) and its relationship with severity of UC.Methods Fifty-two UC patients (18 mild cases,20 moderate cases,and 14 severe cases) and 30 healthy controls were enrolled.Peripheral blood of UC patients and healthy controls was collected.Trizol was used to extract total RNA in peripheral blood monocytes (PBMCs).Real-time quantitative polymerase chain reaction (Real-time PCR) was used to detect CCL20 mRNA expression level in PBMCs.CCL20 protein level in serum was assayed by enzyme-linked immunosorbent assay (ELISA).The difference in CCL20 expression level between two groups was compared by Student' s t test.The difference in CCL20 expression level among mild,moderate and severe UC patients was compared by analysis of variance (ANOVA).Results CCL20 protein expression level in serum of UC patients and healthy controls was (2 040.03 ± 730.07) and (550.01 ± 70.71) ng/L respectively (P ＜ 0.05).CCL20 protein expression in serum of mild,moderate and severe UC patients was (1 233.33± 251.66),(2300.01± 300.00) and (3 034.56 ± 152.75) ng/L,respectively,with the difference being statistically significant (P ＜ 0.05).Conclusion Serum CCL20 expression in UC patients is increased.CCL20 might be involved in the pathogenesis of UC.