中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
6期
1401-1403
,共3页
李辉%曹会存%刘诗义%刘健%曹广劭%赵晓阳
李輝%曹會存%劉詩義%劉健%曹廣劭%趙曉暘
리휘%조회존%류시의%류건%조엄소%조효양
癌,肝细胞%微小RNA-219-5p%乙醇%聚糖蛋白3
癌,肝細胞%微小RNA-219-5p%乙醇%聚糖蛋白3
암,간세포%미소RNA-219-5p%을순%취당단백3
Carcinoma,hepatocellular%MicroRNA-219-5p%Alcohol%Proteoglycan 3
目的 观察乙醇、微小RNA(miRNA)-219-5p对肝细胞肝癌(HCC)中聚糖蛋白3(GPC3)表达的影响.方法 采用免疫组织化学检测30例正常肝脏组织、120例HCC患者肝癌组织及相应癌旁组织标本中GPC3蛋白的表达水平,分析GPC3与HCC汉族临床资料之间的关系,探讨其与饮酒、miRNA-219-5p相关性.结果 GPC3在HCC组织中的阳性表达率为75％,在癌旁组织中其阳性表达率为30％,正常肝组织中未发现其阳性表达,3组比较差异有统计学意义(x2=74.348,P＜0.01).在年龄、乙型肝炎表面抗原(HBsAg)阳性、慢性肝病病史以及肿瘤大小方面,GPC3表达均未受到影响[x2(肝癌组织)=1.800、0.533、1.140、0.568,P＞0.05;x2(癌旁组织)=0.179、0、0、0.714,P＞0.05];在大量饮酒的患者中,GPC3阳性表达率为100％,在非大量饮酒的患者中,其阳性表达率为70％,两组间差异有统计学意义(x2=8.000,P＜0.01),提示饮酒行为对GPC3的表达明显相关.结论 HCC的发生与miRNA-219-5p表达过低、GPC3蛋白表达过高有关,同时大量饮酒行为可能放大了此种效应.
目的 觀察乙醇、微小RNA(miRNA)-219-5p對肝細胞肝癌(HCC)中聚糖蛋白3(GPC3)錶達的影響.方法 採用免疫組織化學檢測30例正常肝髒組織、120例HCC患者肝癌組織及相應癌徬組織標本中GPC3蛋白的錶達水平,分析GPC3與HCC漢族臨床資料之間的關繫,探討其與飲酒、miRNA-219-5p相關性.結果 GPC3在HCC組織中的暘性錶達率為75％,在癌徬組織中其暘性錶達率為30％,正常肝組織中未髮現其暘性錶達,3組比較差異有統計學意義(x2=74.348,P＜0.01).在年齡、乙型肝炎錶麵抗原(HBsAg)暘性、慢性肝病病史以及腫瘤大小方麵,GPC3錶達均未受到影響[x2(肝癌組織)=1.800、0.533、1.140、0.568,P＞0.05;x2(癌徬組織)=0.179、0、0、0.714,P＞0.05];在大量飲酒的患者中,GPC3暘性錶達率為100％,在非大量飲酒的患者中,其暘性錶達率為70％,兩組間差異有統計學意義(x2=8.000,P＜0.01),提示飲酒行為對GPC3的錶達明顯相關.結論 HCC的髮生與miRNA-219-5p錶達過低、GPC3蛋白錶達過高有關,同時大量飲酒行為可能放大瞭此種效應.
목적 관찰을순、미소RNA(miRNA)-219-5p대간세포간암(HCC)중취당단백3(GPC3)표체적영향.방법 채용면역조직화학검측30례정상간장조직、120례HCC환자간암조직급상응암방조직표본중GPC3단백적표체수평,분석GPC3여HCC한족림상자료지간적관계,탐토기여음주、miRNA-219-5p상관성.결과 GPC3재HCC조직중적양성표체솔위75％,재암방조직중기양성표체솔위30％,정상간조직중미발현기양성표체,3조비교차이유통계학의의(x2=74.348,P＜0.01).재년령、을형간염표면항원(HBsAg)양성、만성간병병사이급종류대소방면,GPC3표체균미수도영향[x2(간암조직)=1.800、0.533、1.140、0.568,P＞0.05;x2(암방조직)=0.179、0、0、0.714,P＞0.05];재대량음주적환자중,GPC3양성표체솔위100％,재비대량음주적환자중,기양성표체솔위70％,량조간차이유통계학의의(x2=8.000,P＜0.01),제시음주행위대GPC3적표체명현상관.결론 HCC적발생여miRNA-219-5p표체과저、GPC3단백표체과고유관,동시대량음주행위가능방대료차충효응.
Objective To observe the effects of alcohol and microRNA (miRNA)-219-5p on proteoglycan 3 (GPC3) in hepatocullular carcinoma (HCC).Methods Immunohistochemical staining method was used to detect the expression of 30 cases of normal liver tissues,120 cases of HCC tissues and corresponding para-concerous liver tissues.The relationship between GPC3 and clinical data of Han patients with HCC,and the inner link between GPC3 with alcohol and miRNA-219-5p were analyzed.Results The GPC3 positive expression was 75％ in HCC tissues and 30％ in para-cancerous tissues (x2 =74.348,P ＜0.01).No GPC3 expression was detected in normal liver tissues.The GPC3 expression was not related to age,positive hepatitis B surface antigen (HBsAg),history of chronic liver disease and tumor size [x2(HCC tissues) =1.800,0.533,1.140,0.568,P ＞ 0.05;x2(para-cancerous tissues) =0.179,0,0,0.714,P ＞ 0.05).The GPC3 positive expression rate was 100％ in patients with heavy drinking,and that was 70％ in non-heavy drinking patients.(x2 =8.000,P ＜ 0.01),suggesting drinking behavior had a significant relevance with the GPC3 expression.Conclusion The occurrence of HCC is correlated with the low expression of miRAN-219 and high expression of GPC3 protein.At the same time,heavy drinking behavior can enlarge this effect.
