CAJ | 학술논문

目的通过对大鼠不同肌群乙酰胆碱受体形态学观察和含量测定,探讨自身免疫性重症肌无力肌群易受累的原因.方法分离正常大鼠膈肌、腓肠肌、肋间肌、胸锁乳突肌、心肌、眼肌、咀嚼肌和前肢肌等8组肌群,进行免疫荧光染色,激光共聚焦显微镜观察乙酰胆碱受体(AChR)形态;实时荧光定量聚合酶链反应(FQ-PCR)法比较AChRβ1亚基基因含量.结果 8组肌群均可见红色AChR、绿色神经纤维和突触囊泡;8组肌群中AChRβ1亚基的表达量之间差异有统计学意义(F =395.1,P＜0.05),组间比较腓肠肌(25.49 ±0.31)、肋间肌(24.42±0.75)、胸锁乳突肌(22.20±1.23)、咀嚼肌(11.22 ±0.80)、眼肌(1.46±0.11)之间差异有统计学意义(P＜0.05).结论大鼠各肌群AChR含量的不同与累及不同肌群的重症肌无力发病率明显相关,可部分解释重症肌无力患者发病后肌群受累顺序.
목적 통과대대서불동기군을선담감수체형태학관찰화함량측정,탐토자신면역성중증기무력기군역수루적원인.방법 분리정상대서격기、비장기、륵간기、흉쇄유돌기、심기、안기、저작기화전지기등8조기군,진행면역형광염색,격광공취초현미경관찰을선담감수체(AChR)형태;실시형광정량취합매련반응(FQ-PCR)법비교AChRβ1아기기인함량.결과 8조기군균가견홍색AChR、록색신경섬유화돌촉낭포;8조기군중AChRβ1아기적표체량지간차이유통계학의의(F =395.1,P＜0.05),조간비교비장기(25.49 ±0.31)、륵간기(24.42±0.75)、흉쇄유돌기(22.20±1.23)、저작기(11.22 ±0.80)、안기(1.46±0.11)지간차이유통계학의의(P＜0.05).결론 대서각기군AChR함량적불동여루급불동기군적중증기무력발병솔명현상관,가부분해석중증기무력환자발병후기군수루순서.
Objective To observe acetylcholine receptor (AchR) morphology and content of different muscles in rats,and explore the reasons of myasthenia gravis muscles likely involved.Methods Respectively,we harvested diaphragm,gastrocnemius,intercostal muscle,sternocleidomastoid,myocardial,musculi oculi,cardiac muscle,forelimb in normal rats,and immunofluorescence staining and laser confocal microscopy were used to observe AChR patterns.Results (1) Nerve fibers (green) and AChR (red) could be observed in all the muscles.(2) Among the muscles,AchRβ1 content showed obvious difference (F =395.1,P ＜ 0.05).The content of AchR contents showed an decreased tendency as follows:gastrocnemius (25.49 ± 0.31),intercostal muscle (24.42 ± 0.75),sternocleidomastoid muscle (22.20 ± 1.23),chewing muscle (11.22 ± 0.80),and musculi oculi (1.46 ± 0.11).Conclusion The incidence of rat muscle weakness and its different AChR content has some relevance,which can partialy explain the order of onset of muscle involvement in myasthenia gravis.