CAJ | 학술논문

目的观察帕瑞昔布钠预先给药对大鼠肺缺血再灌注损伤时细胞凋亡的影响.方法健康雄性SD大鼠72只,体质量250～ 300 g,随机分为3组(n=24):假手术组(S组)、肺缺血再灌注组(I/R组)、肺缺血再灌注+帕瑞昔布钠组(P组).阻断右肺门60 min后再灌注120 min制备大鼠肺缺血再灌注模型,P组在夹闭右肺门前30 min腹腔注射帕瑞昔布钠5 mg/kg,S组和I/R组给予等体积生理盐水.于右肺门再灌注2h时处死大鼠,取肺组织.计算肺湿干重比和凋亡指数.免疫组织化学法检测肺组织B细胞淋巴瘤/白血病-2(bcl-2)、B细胞淋巴瘤/白血病-2相关X蛋白(bax)蛋白表达,计算bcl-2/bax比值,并观察病理学结果.结果 I/R组和P组肺组织湿干重比值分别为6.34 ±0.19和5.28 ±0.17、凋亡指数分别为(21.5±2.1)％和(11.6±0.8)％,bcl-2蛋白分别为0.29±0.07和0.45±0.09,bax蛋白分别为0.34 ±0.05和0.18 ±0.04,bcl-2/bax比分别为0.85±0.05和2.50±0.03,两组比较差异均有统计学意义(P＜0.05).结论帕瑞昔布钠预先给药可通过调节bcl-2和bax蛋白的表达抑制细胞凋亡,减轻肺缺血再灌注损伤.
목적 관찰파서석포납예선급약대대서폐결혈재관주손상시세포조망적영향.방법 건강웅성SD대서72지,체질량250～ 300 g,수궤분위3조(n=24):가수술조(S조)、폐결혈재관주조(I/R조)、폐결혈재관주+파서석포납조(P조).조단우폐문60 min후재관주120 min제비대서폐결혈재관주모형,P조재협폐우폐문전30 min복강주사파서석포납5 mg/kg,S조화I/R조급여등체적생리염수.우우폐문재관주2h시처사대서,취폐조직.계산폐습간중비화조망지수.면역조직화학법검측폐조직B세포림파류/백혈병-2(bcl-2)、B세포림파류/백혈병-2상관X단백(bax)단백표체,계산bcl-2/bax비치,병관찰병이학결과.결과 I/R조화P조폐조직습간중비치분별위6.34 ±0.19화5.28 ±0.17、조망지수분별위(21.5±2.1)％화(11.6±0.8)％,bcl-2단백분별위0.29±0.07화0.45±0.09,bax단백분별위0.34 ±0.05화0.18 ±0.04,bcl-2/bax비분별위0.85±0.05화2.50±0.03,량조비교차이균유통계학의의(P＜0.05).결론 파서석포납예선급약가통과조절bcl-2화bax단백적표체억제세포조망,감경폐결혈재관주손상.
Objective To investigate the effects of parecoxib on pneumocyet apoptosis during lung ischemia-reperfusion (I/R) injury in rats.Methods Seventy-two male SD rats were randomly divided into 3 groups (n =24 each):sham operation group (S group);I/R group and parecoxib group (P group).The lung I/R was induced by occlusion of hilum of the right lung for 60 min followed by 120 min of reperfusion.In P group,parecoxib (5 mg/kg) was injected intravenously at 30 min before occlusion of hilum of the right lung.The rats were sacrificed at 2 h of reperfusion and then the lungs were removed for measurement of lung wet/dry weight ratio,apoptosis index,B cell lymphoma/leukemia-2 (bcl-2) and B cell lymphoma/leukemia-2 associated X protein (bax) protein expression,and microscopic examination.Bcl-2/bax ratio was calculated.Results Parecoxib preconditioning significantly attenuated the I/R-induced changes in lung wet/dry weight ratio (6.34 ±0.19 vs.5.28 ±0.17,P ＜0.05),apoptosis index [(21.5 ±2.1)％ vs.(11.6 ±0.8)％,P＜0.05],bcl-2 protein expression (0.29 ±0.07 vs.0.45 ±0.09,P＜0.05) and bax protein expression (0.34 ±0.05 vs.0.18 ±0.04,P＜0.05),and bcl-2/bax ratio (0.85 ± 0.05 vs.2.50 ± 0.03,P ＜ 0.05) in P group as compared with I/R group.Parecoxib preconditioning also ameliorated I/R-induced lung damage.Conclusion Parecoxib can inhibit pneumocyte apoptosis through regulating the expression of bcl-2 and bax protein,and ameliorate lung I/R injury.