CAJ | 학술논문

目的：研究福建汉族人群D1S1656的遗传多态性及其在亲权鉴定中的应用价值。方法应用荧光标记复合扩增系统对日常检案中收集的521名福建汉族无关个体血样DNA进行PCR扩增，用ABI 3130基因分析仪对扩增产物进行毛细管电泳，用GeneMapper v3.1软件进行基因分型，统计分析D1S1656基因座的多态信息，并与其他5个群体进行比较。结果在D1S1656基因座发现17个等位基因，在福建汉族人群中的个体识别能力为0.948，非父排除率为0.665。结论D1S1656基因座在福建汉族人群具有高度多态性，在亲权鉴定中具有重要应用价值。
목적：연구복건한족인군D1S1656적유전다태성급기재친권감정중적응용개치。방법응용형광표기복합확증계통대일상검안중수집적521명복건한족무관개체혈양DNA진행PCR확증，용ABI 3130기인분석의대확증산물진행모세관전영，용GeneMapper v3.1연건진행기인분형，통계분석D1S1656기인좌적다태신식，병여기타5개군체진행비교。결과재D1S1656기인좌발현17개등위기인，재복건한족인군중적개체식별능력위0.948，비부배제솔위0.665。결론D1S1656기인좌재복건한족인군구유고도다태성，재친권감정중구유중요응용개치。
Objective To investigate the genetic polymorphism of D1S1656 STR loci in Han population from Fujian province and to study its application in paternity testing. Methods Fluorescent dye labeling multiplex STR-PCR,capillary electrophoresis and GeneMapper v3.1 software were adopted in genotyping 521 unrelated samples. D1S6156 STR database was established and ana-lyzed.Population comparison was conducted between Han population in Fujian province and 5 other population. Results We ob-served the distribution of D1S1656 STR loci meet Hardy-Weinberge equilibrium and was not different from other 5 population (χ2 test,P>0.05). The power of discrimination was 0.948, and the power of exclusion was 0.665. Conclusion The D1S1656 loci was relatively highly genetic polymorphic in Fujian Han population and could be used for paternity testing.