CAJ | 학술논문

建立一个简便、重现性好测定鸡蛋中的雌二醇、雌酮、雌三醇及睾酮残留量的方法。使用高效液相色谱法，选择流动相为：乙腈∶甲醇∶四氢呋喃∶（0.01 mol/l）醋酸钠=2∶2∶1∶5，能够有效提高色谱峰的分离度，190 nm～800 nm波段下扫描，选择270 nm为最佳吸收波长，柱温：30℃。结果表明4种激素的方法检出限为0.06 mg/kg～0.17 mg/kg，样品回收率是79.23%～102.26%，相对标准偏差为1.05%～4.21%。该方法准确度高，操作简便。
건립일개간편、중현성호측정계단중적자이순、자동、자삼순급고동잔류량적방법。사용고효액상색보법，선택류동상위：을정∶갑순∶사경부남∶（0.01 mol/l）작산납=2∶2∶1∶5，능구유효제고색보봉적분리도，190 nm～800 nm파단하소묘，선택270 nm위최가흡수파장，주온：30℃。결과표명4충격소적방법검출한위0.06 mg/kg～0.17 mg/kg，양품회수솔시79.23%～102.26%，상대표준편차위1.05%～4.21%。해방법준학도고，조작간편。
A efficient method for analysis of estradiol,estrone,estriol,testosterone in eggs was developed. The separation was achieved with a mobile phase composed of acetate sodium (0.01mol/L)-acetonitrile-methanol-tetrahydrofuran (5∶2∶2∶1).The mixed the standard solution of four kinds of anabolic steroids in the 190 nm-800 nm bandwidth scan,selected 270 nm with optimum absorption wavelength. The column was maintained at 30℃. The limit of detection were between 0.06 mg/kg-0.17 mg/kg. Overall recoveries were between 79.23%-102.26%,relative standard deviations between 1.05 %and 4.21 %. The method was suitable to simultaneously analyze the four anabolic steroids in eggs with simplicity and accuracy in a single injection.