中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2015年
5期
332-335
,共4页
有机磷中毒,急性%脑损伤%缺氧,脑%高压氧%丙二醛
有機燐中毒,急性%腦損傷%缺氧,腦%高壓氧%丙二醛
유궤린중독,급성%뇌손상%결양,뇌%고압양%병이철
Organophosphorus poisoning,acute%Brain injury%Hypoxia,brain%Hyperbaric oxygenation%Malondialdehyde
目的 观察急性有机磷中毒大鼠脑组织缺氧诱导因子-1α(HIF-1α)表达的变化,探讨高压氧(HBO)对急性有机磷中毒脑损伤的作用及其机制.方法 健康雄性SD大鼠60只,按随机数字表法分为健康对照组、中毒组、常规治疗组和HBO治疗组,健康对照组6只大鼠,其余每组18只,建立大鼠急性有机磷中毒脑损伤模型,均造模成功.常规治疗组给予长托宁和氯解磷定治疗1次,HBO治疗组在给予常规治疗后即行HBO治疗1次.中毒组、常规治疗组和HBO治疗组分别于造模成功后1、3和7h(每个时间点6只)下腔静脉采血检测丙二醛(MDA)的含量,荧光定量PCR检测脑组织HIF-1 αmRNA的表达,免疫组织化学法检测脑组织HIF-1α蛋白的表达,同时行HE染色观察脑组织病理改变.结果 ①HE染色显示,治疗后HBO治疗组的脑组织病理损伤形态学表现较中毒组有所减轻;②与健康对照组比较,中毒组常规治疗组和HBO治疗组各时间点脑组织HIF-1α蛋白表达明显增高(P＜0.05),各时间点脑组织HIF-1α mRNA相对表达量亦明显增高(P＜0.05);③造模成功后1、3和7h,HBO治疗组各时间点的脑组织HIF-1α的表达分别为(226.57 ± 57.49)、(205.91±30.36)、(187.67±29.25),明显低于较中毒组脑组织HIF-1α的表达[(1305.67±167.17)、(2667.83±367.79)、(1709.24±199.07)],且血清MDA含量[(7.74±0.14)、(7.40±0.13)和(6.10±0.08) nmol/ml]亦较中毒组的血清MDA含量[(9.48±0.05)、(11.56±0.13)和(12.26±0.14) nmol/ml]明显下降,组间差异均有统计学意义(P＜0.05);而HBO治疗组治疗后1h和3h时间点的上述指标低于同时间点常规治疗组,组间差异亦有统计学意义(P＜0.05);④线性相关分析表明,HIF-1α mRNA相对表达量与血清MDA含量具有显著的正相关性(r=0.909,P=0.000).结论 HIF-1α参与了急性有机磷中毒性脑损伤的病理生理过程,HBO对急性有机磷中毒性脑损伤早期的保护作用优于常规治疗组,其作用机制与抗氧化损伤和抑制HIF-1α的表达有关.
目的 觀察急性有機燐中毒大鼠腦組織缺氧誘導因子-1α(HIF-1α)錶達的變化,探討高壓氧(HBO)對急性有機燐中毒腦損傷的作用及其機製.方法 健康雄性SD大鼠60隻,按隨機數字錶法分為健康對照組、中毒組、常規治療組和HBO治療組,健康對照組6隻大鼠,其餘每組18隻,建立大鼠急性有機燐中毒腦損傷模型,均造模成功.常規治療組給予長託寧和氯解燐定治療1次,HBO治療組在給予常規治療後即行HBO治療1次.中毒組、常規治療組和HBO治療組分彆于造模成功後1、3和7h(每箇時間點6隻)下腔靜脈採血檢測丙二醛(MDA)的含量,熒光定量PCR檢測腦組織HIF-1 αmRNA的錶達,免疫組織化學法檢測腦組織HIF-1α蛋白的錶達,同時行HE染色觀察腦組織病理改變.結果 ①HE染色顯示,治療後HBO治療組的腦組織病理損傷形態學錶現較中毒組有所減輕;②與健康對照組比較,中毒組常規治療組和HBO治療組各時間點腦組織HIF-1α蛋白錶達明顯增高(P＜0.05),各時間點腦組織HIF-1α mRNA相對錶達量亦明顯增高(P＜0.05);③造模成功後1、3和7h,HBO治療組各時間點的腦組織HIF-1α的錶達分彆為(226.57 ± 57.49)、(205.91±30.36)、(187.67±29.25),明顯低于較中毒組腦組織HIF-1α的錶達[(1305.67±167.17)、(2667.83±367.79)、(1709.24±199.07)],且血清MDA含量[(7.74±0.14)、(7.40±0.13)和(6.10±0.08) nmol/ml]亦較中毒組的血清MDA含量[(9.48±0.05)、(11.56±0.13)和(12.26±0.14) nmol/ml]明顯下降,組間差異均有統計學意義(P＜0.05);而HBO治療組治療後1h和3h時間點的上述指標低于同時間點常規治療組,組間差異亦有統計學意義(P＜0.05);④線性相關分析錶明,HIF-1α mRNA相對錶達量與血清MDA含量具有顯著的正相關性(r=0.909,P=0.000).結論 HIF-1α參與瞭急性有機燐中毒性腦損傷的病理生理過程,HBO對急性有機燐中毒性腦損傷早期的保護作用優于常規治療組,其作用機製與抗氧化損傷和抑製HIF-1α的錶達有關.
목적 관찰급성유궤린중독대서뇌조직결양유도인자-1α(HIF-1α)표체적변화,탐토고압양(HBO)대급성유궤린중독뇌손상적작용급기궤제.방법 건강웅성SD대서60지,안수궤수자표법분위건강대조조、중독조、상규치료조화HBO치료조,건강대조조6지대서,기여매조18지,건립대서급성유궤린중독뇌손상모형,균조모성공.상규치료조급여장탁저화록해린정치료1차,HBO치료조재급여상규치료후즉행HBO치료1차.중독조、상규치료조화HBO치료조분별우조모성공후1、3화7h(매개시간점6지)하강정맥채혈검측병이철(MDA)적함량,형광정량PCR검측뇌조직HIF-1 αmRNA적표체,면역조직화학법검측뇌조직HIF-1α단백적표체,동시행HE염색관찰뇌조직병리개변.결과 ①HE염색현시,치료후HBO치료조적뇌조직병리손상형태학표현교중독조유소감경;②여건강대조조비교,중독조상규치료조화HBO치료조각시간점뇌조직HIF-1α단백표체명현증고(P＜0.05),각시간점뇌조직HIF-1α mRNA상대표체량역명현증고(P＜0.05);③조모성공후1、3화7h,HBO치료조각시간점적뇌조직HIF-1α적표체분별위(226.57 ± 57.49)、(205.91±30.36)、(187.67±29.25),명현저우교중독조뇌조직HIF-1α적표체[(1305.67±167.17)、(2667.83±367.79)、(1709.24±199.07)],차혈청MDA함량[(7.74±0.14)、(7.40±0.13)화(6.10±0.08) nmol/ml]역교중독조적혈청MDA함량[(9.48±0.05)、(11.56±0.13)화(12.26±0.14) nmol/ml]명현하강,조간차이균유통계학의의(P＜0.05);이HBO치료조치료후1h화3h시간점적상술지표저우동시간점상규치료조,조간차이역유통계학의의(P＜0.05);④선성상관분석표명,HIF-1α mRNA상대표체량여혈청MDA함량구유현저적정상관성(r=0.909,P=0.000).결론 HIF-1α삼여료급성유궤린중독성뇌손상적병리생리과정,HBO대급성유궤린중독성뇌손상조기적보호작용우우상규치료조,기작용궤제여항양화손상화억제HIF-1α적표체유관.
Objective To study the expression of hypoxia-inducible factor-1 (HIF-1) in the brain tissue after the brain injury caused by acute organophosphate poisoning,and the interventional effect and mechanism of hyperbaric oxygen (HBO) therapy.Methods Sixty healthy male Sprague-Dawley rats randomly divided into a control group (n=6),a poisoning group (n=18),a routine group (n=18) and an HBO group (n=18) according to a random number table.Acute organophosphate poisoning was induced into all rats except those in the control group.The routine group was given penehyclidine hydrochloride and pralidoxime chloride for once,while the HBO group was provided with HBO therapy immediately on the basis of routine treatment.At 1,3 and 7 hours after acute organophosphate poisoning was induced,six rats were sacrificed at each time point and the blood samples were taken from inferior caval vein to measure the content of Malondialdehyde (MDA).The expression of HIF-1α mRNA in the brain tissue was detected by the quantitative real-time PCR,and that of HIF-1 protein was evaluated by immunohistochemical method.Meanwhile,pathologic changes of the brain tissues were observed using hematoxylin-eosin (HE) staining.Results Compared with the poisoning group,the pathological damage to cerebral tissues lessened in the HBO group.The expression of HIF-1 protein and HIF-1 mRNA of the poisoning and the HBO groups was significantly higher than the control group at 3 different time points.After the HBO treatment,the protein expression of HIF-1 lowered from 226.57 ± 57.49,to 205.91 ± 30.36 and further to 187.67 ± 29.25,while the MDA content decreased from 7.74 ± 0.14,to 7.40 ± 0.13 and later to 6.10 ±0.08,both were significantly lower than those of the poisoning group at all time points,with HIF-1 being 1305.67 ± 167.17,2667.83 ± 367.79 and 1709.24 ± 199.07,along with MDA content being 9.48 ± 0.05,11.56 ± 0.13 and 12.26 ± 0.14,and those in the routine group at the time points of 1 and 3 hour later (P ＜ 0.05).A positive correlation was found between the expression level of HIF-1 mRNA and level of MDA in the serum (r =0.909,P=0.000).Conclusion HIF-1 plays an important role in the development of brain injury caused by acute organophosphate poisoning.The efficacy of hyperbaric oxygen intervention against AOPP-induced brain injury is better than that of the routine treatment and its mechanism may be its antioxidation and inhibition of HIF-1 expression.
