中国医科大学学报
中國醫科大學學報
중국의과대학학보
JOURNAL OF CHINA MEDICAL UNIVERSITY
2015年
6期
562-564,572
,共4页
髓样分化因子88%ST2825%重组耻垢分枝杆菌
髓樣分化因子88%ST2825%重組恥垢分枝桿菌
수양분화인자88%ST2825%중조치구분지간균
myeloid differentiation factor 88%ST2825%recombinant mycobacterium smegmatis
目的:探讨髓样分化因子88抑制剂ST2825对重组耻垢分枝杆菌感染THP?1细胞自噬的影响。方法使用髓样分化因子88抑制剂ST2825作用于重组耻垢分枝杆菌感染的THP?1细胞,确定ST2825干预的实验组、无ST2825作用的对照组以及空白组。运用荧光显微镜观察自噬小体,RT?PCR检测Beclin?1基因和Bcl?2基因的mRNA的表达。结果与对照组比较,实验组的自噬荧光小点数目明显减少,差异具有统计学意义(P<0.05);RT?PCR检测结果显示Beclin?1和Bcl?2 mRNA表达较对照组下调。结论髓样分化因子88抑制剂ST2858可能通过干扰Beclin?1和Bcl?2的分离,抑制THP?1细胞自噬。
目的:探討髓樣分化因子88抑製劑ST2825對重組恥垢分枝桿菌感染THP?1細胞自噬的影響。方法使用髓樣分化因子88抑製劑ST2825作用于重組恥垢分枝桿菌感染的THP?1細胞,確定ST2825榦預的實驗組、無ST2825作用的對照組以及空白組。運用熒光顯微鏡觀察自噬小體,RT?PCR檢測Beclin?1基因和Bcl?2基因的mRNA的錶達。結果與對照組比較,實驗組的自噬熒光小點數目明顯減少,差異具有統計學意義(P<0.05);RT?PCR檢測結果顯示Beclin?1和Bcl?2 mRNA錶達較對照組下調。結論髓樣分化因子88抑製劑ST2858可能通過榦擾Beclin?1和Bcl?2的分離,抑製THP?1細胞自噬。
목적:탐토수양분화인자88억제제ST2825대중조치구분지간균감염THP?1세포자서적영향。방법사용수양분화인자88억제제ST2825작용우중조치구분지간균감염적THP?1세포,학정ST2825간예적실험조、무ST2825작용적대조조이급공백조。운용형광현미경관찰자서소체,RT?PCR검측Beclin?1기인화Bcl?2기인적mRNA적표체。결과여대조조비교,실험조적자서형광소점수목명현감소,차이구유통계학의의(P<0.05);RT?PCR검측결과현시Beclin?1화Bcl?2 mRNA표체교대조조하조。결론수양분화인자88억제제ST2858가능통과간우Beclin?1화Bcl?2적분리,억제THP?1세포자서。
Objective To investigate the effect of myeloid differentiation factor 88 inhibitor ST2825 on the autophagy of THP?1 cells infected by re?combinant mycobacterium smegmatis. Methods The myeloid differentiation factor 88 inhibitor ST2825 was applied on the THP?1 cells infected by recombinant mycobacterium smegmatis,and three groups were defined:the test group with ST2825 treatment,the control group without ST2825 treatment,and the blank group. Autophagosomes were observed under the fluorescence microscope,and the mRNA expression of Beclin?1 gene and Bcl?2gene was analyzed by RT?PCR. Results Compared with the control group,the number of autophagy fluorescent dots in the test group was ob?viously reduced(P<0. 05),and the expression levels of Beclin 1 gene and Bcl?2 gene were declined as indicated by the RT?PCR detection. Con?clusion The myeloid differentiation factor 88 inhibitor ST2825 might inhibit the autophagy of THP?1 cells through interfering the separation of Be?clin?1 and Bcl?2.