CAJ | 학술논문

目的：研究外周血中分泌型磷脂酶A2（sPLA2）在未足月胎膜早破及羊膜腔感染中的作用及其意义。方法采用实时荧光定量PCR技术检测30例未足月胎膜早破患者（实验组）分娩发动前后、30例未发生胎膜早破的未足月正常妊娠患者（正常对照组）及30例足月胎膜早破患者（足月对照组）分娩发动后外周血中sPLA2 mRNA的表达水平。未足月胎膜早破患者结束妊娠后取胎膜行病理学检查，以确定有无组织学绒毛膜羊膜炎。结果未分娩发动时sPLA2 mRNA的表达水平实验组为1.079±0.746，正常对照组为0.651±0.481，实验组与同孕周正常对照组相比sPLA2 mRNA的表达水平升高，差异有统计学意义（P=0.011）。分娩发动时外周血中sPLA2 mRNA的表达水平实验组为2.439±0.086，足月对照组为2.575±0.036，实验组与足月对照组相比sPLA2 mRNA的表达水平无统计学差异（P=0.787）。实验组中分娩发动时sPLA2的含量与是否患绒毛膜羊膜炎有关（P=0.008）。结论 sPLA2升高可能参与未足月胎膜早破的发病，并与羊膜腔的感染有关。
목적：연구외주혈중분비형린지매A2（sPLA2）재미족월태막조파급양막강감염중적작용급기의의。방법채용실시형광정량PCR기술검측30례미족월태막조파환자（실험조）분면발동전후、30례미발생태막조파적미족월정상임신환자（정상대조조）급30례족월태막조파환자（족월대조조）분면발동후외주혈중sPLA2 mRNA적표체수평。미족월태막조파환자결속임신후취태막행병이학검사，이학정유무조직학융모막양막염。결과미분면발동시sPLA2 mRNA적표체수평실험조위1.079±0.746，정상대조조위0.651±0.481，실험조여동잉주정상대조조상비sPLA2 mRNA적표체수평승고，차이유통계학의의（P=0.011）。분면발동시외주혈중sPLA2 mRNA적표체수평실험조위2.439±0.086，족월대조조위2.575±0.036，실험조여족월대조조상비sPLA2 mRNA적표체수평무통계학차이（P=0.787）。실험조중분면발동시sPLA2적함량여시부환융모막양막염유관（P=0.008）。결론 sPLA2승고가능삼여미족월태막조파적발병，병여양막강적감염유관。
Objective To explore the role and significance of secretory phospholipase A2(sPLA2)in peripheral blood in preterm premature rup?ture of membranes(pPROM)and infection of amniotic cavity. Methods RT-PCR was used to detect the expression levels of sPLA2 mRNA in pe?ripheral blood of 30 patients with pPROM (experimental group),30 non-full term normal pregnant patients without pPROM (normal control group)and 30 full term patients with PROM(full-term control group)before and after delivery. Fetal membranes were collected at the time of deliv?ery of patients with pPROM for pathologic examination to determine histological chorioamnionitis(HCA). Results The expression levels of sPLA2 mRNA in peripheral blood were 1.079±0.746 and 0.651±0.481 in the experimental group and the normal control group before delivery,respectively, indicating that the expression of sPLA2 mRNA was increased in the experimental group compared with the normal control group(P=0.011). The expression levels of sPLA2 mRNA in peripheral blood were 2.439±0.086 and 2.575±0.036 in the experimental group and the full-term control group at labor onset,respectively,indicating that there was no statistically significant difference in the level of sPLA2 mRNA in peripheral blood between the experimental group and the full-term control group at labor onset(P=0.787). The level of sPLA2 was related to chorioamnionitis in the experi?mental group at labor onset. Conclusion The increase of sPLA2 may participate in the pathogenesis of preterm premature rupture of membranes and is related with the infection of chorioamnionitis.