临床肿瘤学杂志
臨床腫瘤學雜誌
림상종류학잡지
CHINESE CLINICAL ONCOLOGY
2015年
6期
506-511
,共6页
P16INK4A%Brn-3a%端粒酶%C-myc%宫颈癌
P16INK4A%Brn-3a%耑粒酶%C-myc%宮頸癌
P16INK4A%Brn-3a%단립매%C-myc%궁경암
P16INK4A%Brn-3a%Telomerase%C-myc%Cervical carcinoma
目的：探讨P16INK4A、Brn?3a及C?myc基因表达及端粒酶活性在宫颈癌诊断中的价值。方法选取2012年3月至2014年5月接受手术治疗的宫颈癌患者40例，检测宫颈癌组织中P16INK4A、Brn?3a、C?myc的表达情况及端粒酶活性水平。同时选取40例宫颈上皮内瘤样病变（ CIN）组织和28例正常宫颈组织作对比。采用受试者工作特征曲线（ ROC）评价组织中P16INK4A、Brn?3a、原癌基因C?myc及端粒酶活性水平在宫颈癌诊断中的价值。结果宫颈癌组织中P16INK4A、Brn?3a和C?myc的阳性表达率分别为100．0％、95．0％和100．0％，均高于正常宫颈和CIN组织，差异有统计学意义（ P＜0．05）；CIN Ⅱ和CIN Ⅲ组织的P16INK4A阳性表达率高于正常宫颈和CINⅠ组织（ P＜0．05）；正常宫颈组织中Brn?3a和C?myc阳性表达率均为7．14％，明显低于其他组织（P＜0．05）；宫颈癌组织中端粒酶活性水平为（44．38±3．82）U／g，高于其余各组，差异有统计学意义（P＜0．05）。 Brn?3a、C?myc诊断宫颈癌的曲线下面积Az＞0．8，且灵敏度和特异度均在80．0％以上，而P16INK4A及端粒酶活性水平的诊断效能相对较低，但4个指标联合检测的诊断效能获提高，灵敏度和特异度分别为89．5％和83．4，曲线下面积Az为0．879。结论宫颈癌组织中P16INK4A、Brn?3a、C?myc表达水平和端粒酶活性异常，可能与宫颈癌的发生发展有密切关系，可作为宫颈癌诊断的参考指标。
目的：探討P16INK4A、Brn?3a及C?myc基因錶達及耑粒酶活性在宮頸癌診斷中的價值。方法選取2012年3月至2014年5月接受手術治療的宮頸癌患者40例，檢測宮頸癌組織中P16INK4A、Brn?3a、C?myc的錶達情況及耑粒酶活性水平。同時選取40例宮頸上皮內瘤樣病變（ CIN）組織和28例正常宮頸組織作對比。採用受試者工作特徵麯線（ ROC）評價組織中P16INK4A、Brn?3a、原癌基因C?myc及耑粒酶活性水平在宮頸癌診斷中的價值。結果宮頸癌組織中P16INK4A、Brn?3a和C?myc的暘性錶達率分彆為100．0％、95．0％和100．0％，均高于正常宮頸和CIN組織，差異有統計學意義（ P＜0．05）；CIN Ⅱ和CIN Ⅲ組織的P16INK4A暘性錶達率高于正常宮頸和CINⅠ組織（ P＜0．05）；正常宮頸組織中Brn?3a和C?myc暘性錶達率均為7．14％，明顯低于其他組織（P＜0．05）；宮頸癌組織中耑粒酶活性水平為（44．38±3．82）U／g，高于其餘各組，差異有統計學意義（P＜0．05）。 Brn?3a、C?myc診斷宮頸癌的麯線下麵積Az＞0．8，且靈敏度和特異度均在80．0％以上，而P16INK4A及耑粒酶活性水平的診斷效能相對較低，但4箇指標聯閤檢測的診斷效能穫提高，靈敏度和特異度分彆為89．5％和83．4，麯線下麵積Az為0．879。結論宮頸癌組織中P16INK4A、Brn?3a、C?myc錶達水平和耑粒酶活性異常，可能與宮頸癌的髮生髮展有密切關繫，可作為宮頸癌診斷的參攷指標。
목적：탐토P16INK4A、Brn?3a급C?myc기인표체급단립매활성재궁경암진단중적개치。방법선취2012년3월지2014년5월접수수술치료적궁경암환자40례，검측궁경암조직중P16INK4A、Brn?3a、C?myc적표체정황급단립매활성수평。동시선취40례궁경상피내류양병변（ CIN）조직화28례정상궁경조직작대비。채용수시자공작특정곡선（ ROC）평개조직중P16INK4A、Brn?3a、원암기인C?myc급단립매활성수평재궁경암진단중적개치。결과궁경암조직중P16INK4A、Brn?3a화C?myc적양성표체솔분별위100．0％、95．0％화100．0％，균고우정상궁경화CIN조직，차이유통계학의의（ P＜0．05）；CIN Ⅱ화CIN Ⅲ조직적P16INK4A양성표체솔고우정상궁경화CINⅠ조직（ P＜0．05）；정상궁경조직중Brn?3a화C?myc양성표체솔균위7．14％，명현저우기타조직（P＜0．05）；궁경암조직중단립매활성수평위（44．38±3．82）U／g，고우기여각조，차이유통계학의의（P＜0．05）。 Brn?3a、C?myc진단궁경암적곡선하면적Az＞0．8，차령민도화특이도균재80．0％이상，이P16INK4A급단립매활성수평적진단효능상대교저，단4개지표연합검측적진단효능획제고，령민도화특이도분별위89．5％화83．4，곡선하면적Az위0．879。결론궁경암조직중P16INK4A、Brn?3a、C?myc표체수평화단립매활성이상，가능여궁경암적발생발전유밀절관계，가작위궁경암진단적삼고지표。
Objective To explore the value of gene expression of P16INK4A , Brn?3a and C?myc as well as the activity of te?lomerase in diagnosis of cervical carcinoma. Methods Cancer tissues from 40 cases of cervical cancer undergoing surgical treatment in our hospital from 2012 March to 2014 May were collected for the detection of P16INK4A , Brn?3a, C?myc and telomerase. Meanwhile, cervical tissues from 28 cases of normal cervical patients and 40 cases of cervical intraepithelial neoplasia were chosen for comparison. The receiver operating characteristic curve ( ROC) was employed to evaluate the diagnostic value of gene expression of P16INK4A , Brn?3a and C?myc as well as the activity of telomerase on cervical carcinoma. Results The positive expression rates of P16INK4A , Brn?3a and C?myc were 100. 0%, 95. 0% and 100. 0% in cervical cancer, significantly higher than those in normal cervical and CIN tissues with statistical difference ( P<0. 05) . P16INK4A positive expression rates of CIN II?III were higher than those of the normal cervical and CIN I tissues ( P<0. 05) . The positive expression rates of Brn?3a and C?myc in normal cervical tissues were 7. 1%, significantly lower than those of other groups (P<0. 05).The level of telomerase activity in tissues from cervical cancer is (44. 38±3. 82) U/g, higher than other groups ( P<0. 05) . Conclusion There were abnormal pattern of gene expression of P16INK4A , Brn?3a and C?myc as well as activity of telomerase, likely to be associated with the pathogenesis and development of cervical cancer. The above parameters can be taken as reference indices for the diagnose of cervical cancer.
