CAJ | 학술논문

目的：探讨孕激素对激素依赖性乳腺癌细胞增殖的影响，观察不同水平维生素D以及孕激素对激素依赖性乳腺癌细胞系T?47D细胞增殖的共同作用及二者之间的相互作用。方法将培养的T?47D细胞分为单纯高、低浓度孕激素处理组，观察孕激素对激素依赖性乳腺癌细胞增殖的影响；设单纯高、低浓度维生素D处理组，观察维生素D对T?47D细胞增殖的影响；设高、低浓度孕激素与高、低浓度维生素D处理组，采用两因素两水平平行析因实验设计，观察不同水平维生素D及孕激素对T?47D细胞增殖的共同作用，对照组为正常培养乳腺癌细胞。采用MTT法检测各组吸光度，观察细胞生长凋亡情况。结果高、低浓度孕激素处理组细胞浓度均显著高于对照组，且低浓度孕激素处理组细胞浓度显著高于高浓度孕激素处理组（P<0.05）；双低组（低浓度维生素D、低浓度孕激素处理组）细胞浓度显著低于其他3个双因素处理组及对照组（P<0.05）。结论孕激素对激素依赖性乳腺癌细胞增殖有促进作用。维生素D和孕激素存在相互作用，且这种相互作用根据二者的浓度不同而有所变化，提示孕激素对乳腺癌细胞的双向作用可能与孕激素和（或）其他因素的浓度有关，但其具体机制尚不明确。
목적：탐토잉격소대격소의뢰성유선암세포증식적영향，관찰불동수평유생소D이급잉격소대격소의뢰성유선암세포계T?47D세포증식적공동작용급이자지간적상호작용。방법장배양적T?47D세포분위단순고、저농도잉격소처리조，관찰잉격소대격소의뢰성유선암세포증식적영향；설단순고、저농도유생소D처리조，관찰유생소D대T?47D세포증식적영향；설고、저농도잉격소여고、저농도유생소D처리조，채용량인소량수평평행석인실험설계，관찰불동수평유생소D급잉격소대T?47D세포증식적공동작용，대조조위정상배양유선암세포。채용MTT법검측각조흡광도，관찰세포생장조망정황。결과고、저농도잉격소처리조세포농도균현저고우대조조，차저농도잉격소처리조세포농도현저고우고농도잉격소처리조（P<0.05）；쌍저조（저농도유생소D、저농도잉격소처리조）세포농도현저저우기타3개쌍인소처리조급대조조（P<0.05）。결론잉격소대격소의뢰성유선암세포증식유촉진작용。유생소D화잉격소존재상호작용，차저충상호작용근거이자적농도불동이유소변화，제시잉격소대유선암세포적쌍향작용가능여잉격소화（혹）기타인소적농도유관，단기구체궤제상불명학。
Objective To study the influence of progesterone on the cell proliferation of hormone?dependent breast cancer and observe the co?effect of vitamin D and progesterone of different levels on the proliferation of the cell line T?47D in hormone?dependent breast cancer and the interaction be?tween vitamin D and progesterone. Methods The cultured T?47D cells were divided into the high and the low progesterone mono?treated groups to observe the effects of different levels of progesterone on the cell proliferation of hormone?dependent breast cancer cell line(T?47D). The high and the low vitamin D mono?treated groups were set to observe the effects of different levels of vitamin D on the cell proliferation. Groups treated by differ?ent levels of progesterone combined with vitamin D were set to observe the interaction between them. Normal breast cancer cells were set as the con?trol group. The two?factor two?level parallel factorial experiment was conducted to observe the co?effect of different levels of progesterone and vitamin D on the proliferation of T?47D. The growth and apoptosis of cells was observed through detection of absorbance in each group by MTT. Results The cell concentration in high and low progesterone treated groups was increased than that in the control group,and was increased in the low proges?terone treated group compared with the high progesterone treated group(P<0.05). The cell concentration in dual?low level treated group was de?creased than that in the other three groups which were two?factor treated and the control group(P<0.05). Conclusion Progesterone stimulates the cell proliferation of hormone?dependent breast cancer. The concentration?based interaction between vitamin D and progesterone indicates that the bi?directional effect of progesterone on breast cancer cells may be related to the concentration of progesterone and other factors,but the specific interac?tion and the mechanism is unclear.