食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2015年
10期
85-89
,共5页
γ-氨基丁酸%比色法%溴甲酚绿
γ-氨基丁痠%比色法%溴甲酚綠
γ-안기정산%비색법%추갑분록
γ-Aminobutyric acid%colorimetric method%bromcresol green
当有乙醛酸、琥珀酸存在时，γ-氨基丁酸（GABA）与溴甲酚绿发生专一的、特殊的显色反应。基于这一显色反应，建立了一种新的快速测定GABA含量的比色法。显色剂中溴甲酚绿、琥珀酸和乙醛酸溶液的适宜浓度分别为0.5、4.0 mg/mL和6.0 mg/mL。测定GABA含量的适宜条件为：4 mL样品溶液，加入2 mL显色剂，混合摇匀，在25℃下水浴5 min，于617 nm测定吸光值。其它氨基酸如谷氨酸对GABA测定有一定的影响。
噹有乙醛痠、琥珀痠存在時，γ-氨基丁痠（GABA）與溴甲酚綠髮生專一的、特殊的顯色反應。基于這一顯色反應，建立瞭一種新的快速測定GABA含量的比色法。顯色劑中溴甲酚綠、琥珀痠和乙醛痠溶液的適宜濃度分彆為0.5、4.0 mg/mL和6.0 mg/mL。測定GABA含量的適宜條件為：4 mL樣品溶液，加入2 mL顯色劑，混閤搖勻，在25℃下水浴5 min，于617 nm測定吸光值。其它氨基痠如穀氨痠對GABA測定有一定的影響。
당유을철산、호박산존재시，γ-안기정산（GABA）여추갑분록발생전일적、특수적현색반응。기우저일현색반응，건립료일충신적쾌속측정GABA함량적비색법。현색제중추갑분록、호박산화을철산용액적괄의농도분별위0.5、4.0 mg/mL화6.0 mg/mL。측정GABA함량적괄의조건위：4 mL양품용액，가입2 mL현색제，혼합요균，재25℃하수욕5 min，우617 nm측정흡광치。기타안기산여곡안산대GABA측정유일정적영향。
γ-aminobutyric acid (GABA) had a unique reaction with bromcresol green when glyoxylic acid and succinic acid were present with them. A new colorimetric method for rapid determination of GABA was developed based on the reaction above. The optimal concentrations of bromcresol green , glyoxylic acid and succinic acid in the chromogenic agent were 0.5, 4.0 mg/mL and 6.0 mg/mL respectively. The optimal condition for determination of GABA was that 4 mL sample was mixed with 2 mL chromogenic agent , and the absorbance of the mixed solution was determined in a spectrophotometer at 617 nm after it was kept in a water bath at 25℃for 5 min. It was found that other amino acids such as glutamate acid had some disturbance.
