CAJ | 학술논문

目的：观察大鼠过敏性休克死亡后血清IgE、类胰蛋白酶的变化规律，探讨其与死亡时间（PMI）、尸体及样本保存环境的相关性。方法建立大鼠过敏性休克死亡动物模型并分组，包括室温组、冷藏组、冷冻组、人工溶血组、血清样本保存组，并设立对照组。大鼠处死采血后，按离心后上层血清颜色进行溶血程度分级。通过ELISA法检测血清IgE、类胰蛋白酶在各组中的质量浓度。结果大鼠过敏性休克死亡后血清IgE、类胰蛋白酶水平明显高于对照组；室温、冷冻保存下血清IgE、类胰蛋白酶随PMI的不同有明显变化，冷藏保存下浓度相对稳定；随溶血程度的增加，血清IgE、类胰蛋白酶水平均呈升高趋势。样本不同温度条件下保存25 d，血清IgE、类胰蛋白酶无明显变化。结论大鼠过敏性休克死后血清IgE、类胰蛋白酶明显升高，但其水平受PMI、环境温度影响较大，尤其是室温或冷冻保存。
목적：관찰대서과민성휴극사망후혈청IgE、류이단백매적변화규률，탐토기여사망시간（PMI）、시체급양본보존배경적상관성。방법건립대서과민성휴극사망동물모형병분조，포괄실온조、랭장조、냉동조、인공용혈조、혈청양본보존조，병설립대조조。대서처사채혈후，안리심후상층혈청안색진행용혈정도분급。통과ELISA법검측혈청IgE、류이단백매재각조중적질량농도。결과대서과민성휴극사망후혈청IgE、류이단백매수평명현고우대조조；실온、냉동보존하혈청IgE、류이단백매수PMI적불동유명현변화，랭장보존하농도상대은정；수용혈정도적증가，혈청IgE、류이단백매수평균정승고추세。양본불동온도조건하보존25 d，혈청IgE、류이단백매무명현변화。결론대서과민성휴극사후혈청IgE、류이단백매명현승고，단기수평수PMI、배경온도영향교대，우기시실온혹냉동보존。
Objective To explore the changes of serum IgE and tryptase caused by anaphylactic shock rats and discuss the relation to PMI and preservative environm ent of corpse and specim en. Methods Rats were used for establishing anaphylactic shock m odels and random ly divided into room tem perature group, refrigeration group, frozen group, manual hem olysis group, specim en preservation group. And the control group was also established. The blood sam ples were collected after rats were sacrificed. The de-gree of hem olysis was graded according to the color of the upper layer of the serum . The mass concen-tration of IgE and tryptase in each group was detected by ELISA. Results The levels of serum IgE and tryptase in anaphylactic shock dead rats were higher than that of the control group. Room tem perature and frozen m ade obviously differences on the levels of serum IgE and tryptase with various PMI. The levels of serum IgE and tryptase in refrigeration group show ed relatively stable. The levels of serum tryptase and IgE were elevated with differently increasing hem olysis. The levels of serum IgE and tryptase show ed no obvious changes during the specim en kept under different tem perature conditions for 25 days. Conclusion Serum IgE and tryptase obviously increased in anaphylactic shock rats. H ow ever, the levels were influenced by PMI and environm ental tem perature, especially under the conditions of room tem perature and frozen.