CAJ | 학술논문

目的：研究骨形态发生蛋白-2（BMP-2）促进骨质疏松性成骨细胞成骨过程中血管内皮生长因子（VEGF）的作用机制。方法用最佳浓度的rhBMP-2干预成骨细胞，同时分别加入不同浓度的VEGF反义寡核苷酸（ASODNs）、苏拉明和外源性VEGF，继续培养48 h后检测碱性磷酸酶（ALP）活性，18 d后检测钙结节的形成。结果（1）大鼠全身骨密度：手术前后大鼠全身骨密度分别为（0.179±0.006）、（0.158±0.007）g/cm2，差异有统计学意义（t=4.180， P<0.05）。（2）VEGF ASODNs组和苏拉明组，其钙结节形成和ALP活性分别随其剂量增加而减少；添加外源性VEGF组，其钙结节形成和ALP活性不受其剂量大小影响。结论单独rhBMP-2能提高去卵巢骨质疏松大鼠骨髓间充质干细胞（BMSCs）的体外成骨能力，而VEGF则不行。VEGF在成骨细胞可能分泌某种物质，促进rhBMP-2诱导成骨活性。
목적：연구골형태발생단백-2（BMP-2）촉진골질소송성성골세포성골과정중혈관내피생장인자（VEGF）적작용궤제。방법용최가농도적rhBMP-2간예성골세포，동시분별가입불동농도적VEGF반의과핵감산（ASODNs）、소랍명화외원성VEGF，계속배양48 h후검측감성린산매（ALP）활성，18 d후검측개결절적형성。결과（1）대서전신골밀도：수술전후대서전신골밀도분별위（0.179±0.006）、（0.158±0.007）g/cm2，차이유통계학의의（t=4.180， P<0.05）。（2）VEGF ASODNs조화소랍명조，기개결절형성화ALP활성분별수기제량증가이감소；첨가외원성VEGF조，기개결절형성화ALP활성불수기제량대소영향。결론단독rhBMP-2능제고거란소골질소송대서골수간충질간세포（BMSCs）적체외성골능력，이VEGF칙불행。VEGF재성골세포가능분비모충물질，촉진rhBMP-2유도성골활성。
Objective To study the mechanism of vascular endothelial growth factor(VEGF)in bone osteoporotic bone marrow stromal cells (BMSCs) induced bone morphogenetic by protein-2. Methods Optimal concentration protein-2 intervene osteoblasts,While add different concentration respectively VEGF antisense oligodeoxynucleotides(VEGF ASODNs), Suramin and exogenous VEGF. After 48 hours,observe the influence on alkaline phosphatase(ALP) activity .After 18 days,observe the influence on bone nodule formation of osteoblasts. Results ①Whole body bone mineral density of rats:the whole body bone mineral density of rats before and after operation were (0.179±0.007) g/cm2, (0.158 ± 0.006) g/cm2. Difference was statistically significant (t=4.180, P<0.05). ② The alkaline phosphatase(ALP) activity and bone nodule formation of osteoblasts reduce respectively With the increase of VEGF ASODNs and Suramin ,yet unaffect practically With the increase of exogenous VEGF. Conclusion Alonely RhBMP-2 can improve to ovary osteoporosis rat BMSCs in vitro osteogenesis ability, yet VEGF cannot. VEGF acts in an autocrine manner on the osteoblastic cells, at least partly in the process of osteogenesis induced by rhBMP-2.