CAJ | 학술논문

采用室内培养实验，首先用0~300μg·L-1砷酸盐[As（Ⅴ）]处理钝顶螺旋藻（Spirulina platensis），分析了螺旋藻对As（Ⅴ）的吸附和吸收特性，并在300μg·L-1 As（Ⅴ）处理下，研究了不同磷浓度（P 正、1/10 P 正、1/25 P 正、1/50 P 正）对螺旋藻吸附、吸收和转化As （Ⅴ）的影响。结果表明，在本研究As（Ⅴ）处理范围内，螺旋藻的干重与对照没有显著差异。随着As（Ⅴ）浓度的升高，藻体富集的总砷含量增加，当As（Ⅴ）处理浓度为150~300μg·L-1时砷富集量为1.006~1.569 mg·kg-1，超过了国家保健（功能）食品的砷污染限量1.0 mg·kg-1（GB 16740—1997）。随着培养基中磷浓度的降低，螺旋藻体内吸收的砷含量呈现增多趋势。在正常磷（P 正）和1/10 P 正条件下，螺旋藻体内的砷均为As（Ⅴ）；当磷浓度降低至1/25 P 正时，藻细胞中的砷有3.28%为As（Ⅲ）；当磷浓度降低为1/50 P 正时，螺旋藻吸收的砷增加至1.457 mg·kg-1，其中有9.24%和37.35%分别转化为As（Ⅲ）和二甲基砷（DMA），表明降低培养基中磷浓度促进了螺旋藻体对As（Ⅴ）的吸收、还原和甲基化，但藻细胞中砷的主要形态仍为As（Ⅴ）。在正常磷浓度培养下，藻体富集的砷以藻细胞表面吸附为主，通过磷酸盐缓冲液脱附可去除95%以上螺旋藻富集的砷。
채용실내배양실험，수선용0~300μg·L-1신산염[As（Ⅴ）]처리둔정라선조（Spirulina platensis），분석료라선조대As（Ⅴ）적흡부화흡수특성，병재300μg·L-1 As（Ⅴ）처리하，연구료불동린농도（P 정、1/10 P 정、1/25 P 정、1/50 P 정）대라선조흡부、흡수화전화As （Ⅴ）적영향。결과표명，재본연구As（Ⅴ）처리범위내，라선조적간중여대조몰유현저차이。수착As（Ⅴ）농도적승고，조체부집적총신함량증가，당As（Ⅴ）처리농도위150~300μg·L-1시신부집량위1.006~1.569 mg·kg-1，초과료국가보건（공능）식품적신오염한량1.0 mg·kg-1（GB 16740—1997）。수착배양기중린농도적강저，라선조체내흡수적신함량정현증다추세。재정상린（P 정）화1/10 P 정조건하，라선조체내적신균위As（Ⅴ）；당린농도강저지1/25 P 정시，조세포중적신유3.28%위As（Ⅲ）；당린농도강저위1/50 P 정시，라선조흡수적신증가지1.457 mg·kg-1，기중유9.24%화37.35%분별전화위As（Ⅲ）화이갑기신（DMA），표명강저배양기중린농도촉진료라선조체대As（Ⅴ）적흡수、환원화갑기화，단조세포중신적주요형태잉위As（Ⅴ）。재정상린농도배양하，조체부집적신이조세포표면흡부위주，통과린산염완충액탈부가거제95%이상라선조부집적신。
Arsenic(As)accumulation in Spirulina platensis may reduce the quality of health products produced from this microalga. Phos-phorus(P)may influence the algal uptake and biotransformation of As. Thus it would be possible to reduce the accumulation of As in algae by manipulating P concentrations in growth media. To test this, we first sat up an experiment to examine the As(Ⅴ)adsorption and absorp-tion characteristics of S. platensis under 0~300μg·L-1 As(Ⅴ), and then conducted another experiment to study the effects of normal and low P levels on the As(Ⅴ)uptake, adsorption and biotransformation by S. platensis at 300μg·L-1As(Ⅴ). Algal biomass was not signifi-cantly affected by As(Ⅴ)treatments, compared with the control. However, arsenic accumulation increased with As(Ⅴ)levels in the growth media. At 150~300 μg·L-1 As(Ⅴ), S. platensis contained 1.006 to 1.569 mg·kg-1, which exceeded the national standard(GB 16740—1997)for health(functional)foods(1.0 mg·kg-1). The As absorption was reduced by increasing P concentrations. At P levels of 1/10 normal or higher, P did not change As speciation in S. platensis, whereas only 3.28% of As was transformed to As(Ⅲ)at 1/25 normal P level. When P concentration was lowered to 1/50 of normal P level, As uptake increased significantly to 1.457 mg·kg-1, of which 9.24% and 37.35%were converted to As(Ⅲ)and DMA, respectively. In conclusion, decrease in P supply promoted the uptake, reduction and methyla-tion of As(Ⅴ), though As(Ⅴ)was still the main As species in the microalgae. The majority of As was adsorbed on the surface of algal cells, suggesting that more than 95%of As in the S. platensis could be removed by washing with phosphate buffered saline(PBS)solution.