CAJ | 학술논문

优化了黑曲霉A．niger 6640发酵制备蔗果低聚糖的发酵条件，研究了A．niger 6640中β-呋喃果糖苷转移酶（β-Ffase ）的性质，用阴离子柱DEAE Sepharose CL-6 B对β-Ffase进行了分离纯化，用SDS-PAGE电泳初步分析了β-Ffase的分子质量．结果表明：A． niger 6640发酵制备FOS 的最佳pH 值为7.6、最佳发酵时间为40 h、最佳发酵温度为33℃、最佳蔗糖质量分数为40％；在磷酸氢二钠－柠檬酸缓冲液中，采用真空干燥工艺从A．niger 6640中制得的β-Ffase的催化反应的最佳pH值为6.5、最佳反应时间为16 h、最适酶用量为0.19 g；分离纯化得到的β-Ffase的分子质量约为75 ku．
우화료흑곡매A．niger 6640발효제비자과저취당적발효조건，연구료A．niger 6640중β-부남과당감전이매（β-Ffase ）적성질，용음리자주DEAE Sepharose CL-6 B대β-Ffase진행료분리순화，용SDS-PAGE전영초보분석료β-Ffase적분자질량．결과표명：A． niger 6640발효제비FOS 적최가pH 치위7.6、최가발효시간위40 h、최가발효온도위33℃、최가자당질량분수위40％；재린산경이납－저몽산완충액중，채용진공간조공예종A．niger 6640중제득적β-Ffase적최화반응적최가pH치위6.5、최가반응시간위16 h、최괄매용량위0.19 g；분리순화득도적β-Ffase적분자질량약위75 ku．
In this paper, the preparation conditions of fructooligosaccharides ( FOS) via the biotransformation by Aspergillus niger 6640 ( A.niger 6640) were optimized, and the properties of crude enzyme of β-Ffase from A.ni-ger 6640 were investigated .Then,β-Ffase was isolated and purified by using an anion column of DEAE Sepharose CL-6B, and its molecular mass was preliminarily measured by means of SDS-PAGE electrophoresis.The results show that (1) the optimal conditions for FOS preparation should be controlled at a pH value of 7.6, a fermentation time of 40 h, a fermentation temperature of 33℃ and a sucrose mass fraction of 40%; ( 2 ) in the buffer of Na2 HPO4-citric acid for the biotransformation using vacuum-driedβ-Ffase, the optimal conditions are determined as pH 6.5, a reaction time of 16 h and aβ-Ffase dosage of 0.19 g;and (3) the molecular mass of β-Ffase after isola-tion and purification is about 75 ku.