介入放射学杂志
介入放射學雜誌
개입방사학잡지
JOURNAL OF INTERVENTIONAL RADIOLOGY
2015年
6期
574-578
,共5页
赵真真%王忠敏%陆健%茅爱武%刘芬菊
趙真真%王忠敏%陸健%茅愛武%劉芬菊
조진진%왕충민%륙건%모애무%류분국
125I粒子持续低剂量率照射%60Coγ射线高剂量率照射%非小细胞肺癌%凋亡
125I粒子持續低劑量率照射%60Coγ射線高劑量率照射%非小細胞肺癌%凋亡
125I입자지속저제량솔조사%60Coγ사선고제량솔조사%비소세포폐암%조망
125I seed continuous low dose rate irradiation%60Coγ-ray high dose rate irradiation%non-small cell lung cancer%apoptosis
目的:探讨125I粒子持续低剂量率(CLDR)内照射和60Co γ射线高剂量率(HDR)外照射对非小细胞肺癌(NSCLC)细胞株H1299的细胞生物学效应。方法 H1299细胞处于指数生长期时分别行125I粒子CLDR照射和60Coγ射线HDR照射;克隆形成实验检测细胞存活分数,流式细胞术检测细胞周期和细胞凋亡率,Western blot检测Bax、Bcl-2蛋白表达水平。结果随着照射剂量增大,125I粒子CLDR照射比60Coγ射线HDR照射抑制H1299细胞增殖效应更明显。照射剂量为4 Gy时125I粒子组H1299细胞G2/M期百分比、细胞凋亡率分别为21.77±0.31%、13.79±0.50%;同样照射剂量下,60Co照射组H1299细胞G2/M期百分比仅为18.85±0.99%,细胞凋亡率仅为8.79±0.22%(P<0.05)。125I粒子CLDR照射明显上调Bax蛋白表达,同时下调Bcl-2蛋白表达。结论125I粒子CLDR内照射比60Coγ射线HDR外照射抑制H1299细胞增殖效应更明显。 Bcl-2/Bax蛋白比失衡在125I粒子CLDR照射抗肿瘤效应中可能发挥重要作用。
目的:探討125I粒子持續低劑量率(CLDR)內照射和60Co γ射線高劑量率(HDR)外照射對非小細胞肺癌(NSCLC)細胞株H1299的細胞生物學效應。方法 H1299細胞處于指數生長期時分彆行125I粒子CLDR照射和60Coγ射線HDR照射;剋隆形成實驗檢測細胞存活分數,流式細胞術檢測細胞週期和細胞凋亡率,Western blot檢測Bax、Bcl-2蛋白錶達水平。結果隨著照射劑量增大,125I粒子CLDR照射比60Coγ射線HDR照射抑製H1299細胞增殖效應更明顯。照射劑量為4 Gy時125I粒子組H1299細胞G2/M期百分比、細胞凋亡率分彆為21.77±0.31%、13.79±0.50%;同樣照射劑量下,60Co照射組H1299細胞G2/M期百分比僅為18.85±0.99%,細胞凋亡率僅為8.79±0.22%(P<0.05)。125I粒子CLDR照射明顯上調Bax蛋白錶達,同時下調Bcl-2蛋白錶達。結論125I粒子CLDR內照射比60Coγ射線HDR外照射抑製H1299細胞增殖效應更明顯。 Bcl-2/Bax蛋白比失衡在125I粒子CLDR照射抗腫瘤效應中可能髮揮重要作用。
목적:탐토125I입자지속저제량솔(CLDR)내조사화60Co γ사선고제량솔(HDR)외조사대비소세포폐암(NSCLC)세포주H1299적세포생물학효응。방법 H1299세포처우지수생장기시분별행125I입자CLDR조사화60Coγ사선HDR조사;극륭형성실험검측세포존활분수,류식세포술검측세포주기화세포조망솔,Western blot검측Bax、Bcl-2단백표체수평。결과수착조사제량증대,125I입자CLDR조사비60Coγ사선HDR조사억제H1299세포증식효응경명현。조사제량위4 Gy시125I입자조H1299세포G2/M기백분비、세포조망솔분별위21.77±0.31%、13.79±0.50%;동양조사제량하,60Co조사조H1299세포G2/M기백분비부위18.85±0.99%,세포조망솔부위8.79±0.22%(P<0.05)。125I입자CLDR조사명현상조Bax단백표체,동시하조Bcl-2단백표체。결론125I입자CLDR내조사비60Coγ사선HDR외조사억제H1299세포증식효응경명현。 Bcl-2/Bax단백비실형재125I입자CLDR조사항종류효응중가능발휘중요작용。
Objective To investigate the biological effect of 125I seeds continuous low dose rate (CLDR) irradiation and 60Co γ-ray high dose rate (HDR) irradiation on H1299 cell line of non-small cell lung cancer (NSCLC). Methods H1299 cells in exponential growth were separately irradiated with 125I seeds CLDR irradiation and 60Co γ-ray HDR irradiation. The cell survival fraction was inspected with clone formation experiment, the cell cycle and apoptosis rate was determined with flow cytometry, and the expressions of Bax and Bcl-2 proteins were measured by Western blot method. Results With the irradiation dose increasing, the anti-proliferation effect of 125I seeds CLDR irradiation on H1299 cells became more remarkable than that of 60Coγ-ray HDR irradiation. When the irradiation dose reached 4 Gy, the G2/M phase percentage and the apoptotic ratio of H1299 cells in 125I seeds CLDR irradiation group were 21.77±0.31%and 13.79±0.50% respectively, which were only 18.85±0.99% and 8.79±0.22% respectively in 60Co γ-ray HDR irradiation group, the difference was statistically significant (P<0.05). In 125I seeds CLDR irradiation group the expression of Bax protein was remarkably up-regulated, while the expression of Bcl-2 protein was down-regulated. Conclusion The inhibition effect of 125I seeds CLDR internal irradiation on the proliferation of H1299 cells is more obvious than that of 60Co γ-ray HDR irradiation. In 125I seeds CLDR irradiation group, the imbalance of Bcl-2/Bax ratio may play an important role in achieving the antitumor effect.