CAJ | 학술논문

目的：比较卡式全自动血型仪法与传统血清学法对不规则抗体筛查的应用效果。方法分别采用卡式全自动血型仪法与传统血清学方法对2013年10月1日至2014年4月30日东莞市无偿献血者45709份标本进行不规则抗体筛查，对筛查阳性的标本采用抗球蛋白法进行鉴定，比较两种方法检出不规则抗体的性能，并对献血者不规则抗体分布进行分析。结果卡式全自动血型仪检出不规则抗体阳性95例，检出率为0．208％，传统血清学法检出不规则抗体16例，检出率0．035％，差异有统计学意义（ P＜0．05）；卡式全自动血型仪法阳性检出符合率为56．547％，高于传统血清学法阳性检出符合率28．571％（ P＜0．05）；RhD阴性献血者不规则抗体阳性率为2．242％，高于RhD阳性献血者不规则抗体率0．198％（P＜0．05）。结论卡式全自动血型仪法鉴定献血者红细胞不规则抗体的阳性检出率高于传统血清学法，对RhD阴性献血者更应进行不规则抗体的筛查，献血者红细胞不规则抗体类型以Ig M型无临床意义的抗体为主。
목적：비교잡식전자동혈형의법여전통혈청학법대불규칙항체사사적응용효과。방법분별채용잡식전자동혈형의법여전통혈청학방법대2013년10월1일지2014년4월30일동완시무상헌혈자45709빈표본진행불규칙항체사사，대사사양성적표본채용항구단백법진행감정，비교량충방법검출불규칙항체적성능，병대헌혈자불규칙항체분포진행분석。결과잡식전자동혈형의검출불규칙항체양성95례，검출솔위0．208％，전통혈청학법검출불규칙항체16례，검출솔0．035％，차이유통계학의의（ P＜0．05）；잡식전자동혈형의법양성검출부합솔위56．547％，고우전통혈청학법양성검출부합솔28．571％（ P＜0．05）；RhD음성헌혈자불규칙항체양성솔위2．242％，고우RhD양성헌혈자불규칙항체솔0．198％（P＜0．05）。결론잡식전자동혈형의법감정헌혈자홍세포불규칙항체적양성검출솔고우전통혈청학법，대RhD음성헌혈자경응진행불규칙항체적사사，헌혈자홍세포불규칙항체류형이Ig M형무림상의의적항체위주。
Objective To compare the application effect between the method of cassette automatic blood analyzer and the method of traditional serological .Methods Using the method of cassette automatic blood analyzer and the method of traditional serological , respectively ,the samples of blood donors in Dongguan from October 1 ,2013 to April 30 ,2014 were performed irregular antibody screening .The positive samples of screening were identified by using antiglobulin method ,and the irregular antibodies of blood do‐nors were analyzed .Results There were 95 positive cases of irregular antibody by the method of cassette automatic blood analyzer , and the detection rate was 0 .208% .There were 16 positive cases of irregular antibody by the method of traditional serological ,the detection rate was 0 .035% ,and there was statistical significance in differences (P< 0 .05) .The positive coincidence rate of the method of cassette automatic blood analyzer was 56 .547% ,higher than the rate of the method of traditional serological which was 28 .571 % (P<0 .05) .The positive rate of irregular antibody was 2 .242% in RhD‐negative blood donors ,higher than the rate in RhD‐positive blood donors which was 0 .198% (P<0 .05) .Conclusion The positive rate of irregular antibody in blood donors i‐dentified by using the method of cassette automatic blood analyzer is higher than the rate identified by using the method of tradition‐al serological .The irregular antibody screening should be performed for RhD‐negative blood donors .The types of irregular antibody in blood donors are mainly the types of IgM which are no clinical significance .