CAJ | 학술논문

以吉富罗非鱼GIFT Oreochromis niloticus同一个家系卵黄囊期鱼苗为研究对象,对比36℃水温诱导与浓度为0.6μg/g (饵料)甲基睾酮投喂对其雄性率及生长发育的影响。结果表明：36℃水温持续诱导10 d的平均雄性率为(95.0±1.5)%,而30℃下投喂甲基睾酮10 d的平均雄性率仅为(73.0±2.4)%,投喂20 d后达到(96.0±1.3)%,36℃水温诱导+甲基睾酮投喂10 d的平均雄性率为(95.8±1.8)%,对照组仅为(56.0±2.1)%；后期的生长发育显示,36℃水温诱导+甲基睾酮投喂10 d组的生长速度最快,平均日增重达到了5.42 g/d,显著优于其他组(P<0.05)；性腺组织切片观察显示,经过36℃水温诱导后的雌鱼大部分具有两性特征,无法正常排卵。研究表明,36℃水温诱导10 d能显著提高吉富罗非鱼仔鱼的雄性率,其效果已接近甲基睾酮处理20 d的效果,研究结果将有望应用于吉富罗非鱼苗种生产。
이길부라비어GIFT Oreochromis niloticus동일개가계란황낭기어묘위연구대상,대비36℃수온유도여농도위0.6μg/g (이료)갑기고동투위대기웅성솔급생장발육적영향。결과표명：36℃수온지속유도10 d적평균웅성솔위(95.0±1.5)%,이30℃하투위갑기고동10 d적평균웅성솔부위(73.0±2.4)%,투위20 d후체도(96.0±1.3)%,36℃수온유도+갑기고동투위10 d적평균웅성솔위(95.8±1.8)%,대조조부위(56.0±2.1)%；후기적생장발육현시,36℃수온유도+갑기고동투위10 d조적생장속도최쾌,평균일증중체도료5.42 g/d,현저우우기타조(P<0.05)；성선조직절편관찰현시,경과36℃수온유도후적자어대부분구유량성특정,무법정상배란。연구표명,36℃수온유도10 d능현저제고길부라비어자어적웅성솔,기효과이접근갑기고동처리20 d적효과,연구결과장유망응용우길부라비어묘충생산。
The effects of water temperature of 36 ℃ and diet containing 0. 6 μg/g methyltestosterone on male rate, growth and development of sibling prolarvae were studied in GIFT Nile tilapia Oreochromis niloticus. The results showed that the average male rate of (95. 0±1. 5)% was observed in the fish raised at 36℃ for 10 days. The aver-age male rate of only (73. 0±2. 4)% was found in the fish fed the diet containing 0. 6 μg/g methyltestosterone at 30 ℃ for 10 days. However, the fish fed the diet containing 0. 6μg/g methyltestosterone for 20 days had male rate of up to (96. 0±1. 3)%. The fish fed the diet containing 0. 6 μg/g methyltestosterone at 36 ℃ water for 10 days had male rate of(95. 8±1. 8)%, about 40% higher than the fish in the control group (only 56%) did. The best growth (5. 42 g/d in average)was observed in the GIFT Nile tilapia fed the diet containing 0. 6 μg/g methyltestos-terone at 36 ℃ for 10 days, significantly higher than that the fish in the other groups (P<0. 05). Histologically, most females raised at 36 ℃ exhibited bisexuality without normal ovulation. In conclusion, the male rate is signifi-cantly improved, close to the fish fed the diet containing methyltestosterone at a dose of 0. 6μg/g feed for 20 days, in larval GIFT Nile tilapia reared at 36 ℃ for 10 days. The findings provide clues for the fingerling production of GIFT Nile tilapia.