兰州大学学报（医学版）
蘭州大學學報（醫學版）
란주대학학보（의학판）
JOURNAL OF LANZHOU UNIVERSITY(MEDICAL SCIENCES)
2015年
3期
1-7
,共7页
张艳杰%武敏%蒋贤杰%张渝君
張豔傑%武敏%蔣賢傑%張渝君
장염걸%무민%장현걸%장투군
全长组织因子%肿瘤细胞转移%慢病毒表达载体%RNA干扰%乳腺癌
全長組織因子%腫瘤細胞轉移%慢病毒錶達載體%RNA榦擾%乳腺癌
전장조직인자%종류세포전이%만병독표체재체%RNA간우%유선암
full-length tissue factor%tumor cell migration%lentivirus expression vector%RNA interference%breast cancer
目的用慢病毒表达载体研究全长组织因子（flTF）在乳腺癌发生和发展中的作用。方法包装flTF的慢病毒颗粒感染永生化乳腺上皮细胞株MCF-10A实现flTF的过表达，用flTF shRNA的慢病毒颗粒感染乳腺癌细胞株MDA-MB-231下调flTF的表达，用Transwell的方法检测flTF过表达和下调表达之后对细胞迁移的影响，用流式细胞术检测flTF对细胞周期的影响，蛋白印迹法检测flTF过表达和下调表达的效果，结合MTS方法检测flTF对细胞增殖的影响。结果flTF在乳腺癌细胞株MDA-MB-231中的表达明显高于永生化的乳腺上皮细胞MCF-10A，正常细胞中flTF过表达促进了细胞的迁移并对其生长速度产生了一定的影响。在肿瘤细胞中下调flTF的表达，能在一定程度上抑制肿瘤细胞的迁移和生长。结论慢病毒表达载体在flTF的表达对乳腺癌细胞的影响中得到成功应用， flTF可作为抑制肿瘤细胞生长和迁移的靶分子，下调flTF的表达或许能为乳腺癌的临床治疗开辟一条新的途径。
目的用慢病毒錶達載體研究全長組織因子（flTF）在乳腺癌髮生和髮展中的作用。方法包裝flTF的慢病毒顆粒感染永生化乳腺上皮細胞株MCF-10A實現flTF的過錶達，用flTF shRNA的慢病毒顆粒感染乳腺癌細胞株MDA-MB-231下調flTF的錶達，用Transwell的方法檢測flTF過錶達和下調錶達之後對細胞遷移的影響，用流式細胞術檢測flTF對細胞週期的影響，蛋白印跡法檢測flTF過錶達和下調錶達的效果，結閤MTS方法檢測flTF對細胞增殖的影響。結果flTF在乳腺癌細胞株MDA-MB-231中的錶達明顯高于永生化的乳腺上皮細胞MCF-10A，正常細胞中flTF過錶達促進瞭細胞的遷移併對其生長速度產生瞭一定的影響。在腫瘤細胞中下調flTF的錶達，能在一定程度上抑製腫瘤細胞的遷移和生長。結論慢病毒錶達載體在flTF的錶達對乳腺癌細胞的影響中得到成功應用， flTF可作為抑製腫瘤細胞生長和遷移的靶分子，下調flTF的錶達或許能為乳腺癌的臨床治療開闢一條新的途徑。
목적용만병독표체재체연구전장조직인자（flTF）재유선암발생화발전중적작용。방법포장flTF적만병독과립감염영생화유선상피세포주MCF-10A실현flTF적과표체，용flTF shRNA적만병독과립감염유선암세포주MDA-MB-231하조flTF적표체，용Transwell적방법검측flTF과표체화하조표체지후대세포천이적영향，용류식세포술검측flTF대세포주기적영향，단백인적법검측flTF과표체화하조표체적효과，결합MTS방법검측flTF대세포증식적영향。결과flTF재유선암세포주MDA-MB-231중적표체명현고우영생화적유선상피세포MCF-10A，정상세포중flTF과표체촉진료세포적천이병대기생장속도산생료일정적영향。재종류세포중하조flTF적표체，능재일정정도상억제종류세포적천이화생장。결론만병독표체재체재flTF적표체대유선암세포적영향중득도성공응용， flTF가작위억제종류세포생장화천이적파분자，하조flTF적표체혹허능위유선암적림상치료개벽일조신적도경。
Objective To study the role of full-length tissue factor in initiation and development of breast cancer with lentivirus expression system. Methods Pack full-length tissue factor lentivirus particle and infect human non-tumorigenic immortalized breast epithelial cell line MCF-10A cells to overexpression full-length tissue factorin this cell line. Infect breast cancer cell line MDA-MB-231 with full-length tissue factor's shR-NA particles to knockdown full-length tissue factor. The effects of full-length tissue factor's overexpression and knock-down on cell migration were shown by Transwell assay, cell cycle was detected by Flow cytome-try, full-length tissue factor's overexpression and knockdown were detected by Western blot, the effect of tis-sue factor on cell proliferation was detected by MTS assay. Results The expression of full-length tissue factor in breast cancer cell line MDA-MB-231 was significantly higher than that in human non-tumorigenic immor-talized breast epithelial cell line MCF-10A cells , overexpression of full-length tissue factor in MCF-10A cells increased cell migration and growth, while knockdown of tissue factor in MDA-MB-231 cells, suppressed migration and proliferation of these malignant tumor cells. Conclusion These results suggest that Letivirus expression system was successfully used in the study of full-length tissue factor's role in breast cancer. Therefore, full-length tissue factor could be a target for treatment of breast cancer. Inhibition of full-length <br> tissue factor expression would open up a novel approach to anti-breast cancer therapy.
