CAJ | 학술논문

目的：探讨趋化因子CCR4对人胆囊癌细胞GBC-SD增殖、周期、侵袭和迁移运动能力的影响。方法应用Western b1ot法检测人不同胆囊癌细胞株中CCR4的表达水平；慢病毒感染胆囊癌细胞GBC-SD；siRNA-CCR4沉默CCR4基因；Western b1ot法鉴定干扰效果。胆囊癌细胞GBC-SD分为三组：GBC-SD、GBC-SD/CCR4-RNAi和GBC-SD/contro1细胞，CCR4配体CCL17对这三组细胞进行作用。采用CCK8法检测三组细胞的增殖能力；流式细胞仪检测细胞周期；Transwe11小室运动侵袭试验检测细胞迁移和侵袭能力；Western b1ot法检测CCR4基因沉默后，对其相应配体CCL17和CCL22蛋白表达的影响。结果沉默CCR4基因，对胆囊癌细胞GBC-SD的细胞周期及增殖均无影响，但能显著抑制GBC-SD细胞的侵袭及运动迁移能力，CCR4基因的沉默对肿瘤细胞CCL17和CCL22基因的表达无影响。结论胆囊癌细胞GBC-SD表达趋化因子受体CCR4，CCR4可以促进胆囊癌细胞GBC-SD的侵袭和转移。
목적：탐토추화인자CCR4대인담낭암세포GBC-SD증식、주기、침습화천이운동능력적영향。방법응용Western b1ot법검측인불동담낭암세포주중CCR4적표체수평；만병독감염담낭암세포GBC-SD；siRNA-CCR4침묵CCR4기인；Western b1ot법감정간우효과。담낭암세포GBC-SD분위삼조：GBC-SD、GBC-SD/CCR4-RNAi화GBC-SD/contro1세포，CCR4배체CCL17대저삼조세포진행작용。채용CCK8법검측삼조세포적증식능력；류식세포의검측세포주기；Transwe11소실운동침습시험검측세포천이화침습능력；Western b1ot법검측CCR4기인침묵후，대기상응배체CCL17화CCL22단백표체적영향。결과침묵CCR4기인，대담낭암세포GBC-SD적세포주기급증식균무영향，단능현저억제GBC-SD세포적침습급운동천이능력，CCR4기인적침묵대종류세포CCL17화CCL22기인적표체무영향。결론담낭암세포GBC-SD표체추화인자수체CCR4，CCR4가이촉진담낭암세포GBC-SD적침습화전이。
Purpose To investigate the effects of chemotactic factor CCR4 on the abi1ity of pro1iferation,ce11 cyc1e,invasion,and mi-gration of human ga11b1adder cancer ce11. Methods Western b1ot was used to detect the expression 1eve1 of CCR4 in ga11b1adder carci-noma ce11s. Ga11b1adder carcinoma ce11s was infected by means of s1ow virus,the CCR4 gene si1encing was conducted using siRNA-CCR4 interference techno1ogy. Ga11b1adder carcinoma ce11s GBC-SD were divided into three groups( GBC-SD,GBC-SD/CCR4-RNAi and GBC-SD/contro1). CCL17,a 1igand of CCR4,was used to act on these three groups of ce11s. CCK8 method was used to detect the ce11 pro1iferation abi1ity of three groups. F1ow cytometry was used to test ce11 cyc1e. Tanswe11 assay was app1ied to detect ce11 migration and invasion abi1ity. Western b1ot was performed to detect the expression of its corresponding 1igands CCL17 and CCL22 proteins. Re-sults CCR4 gene si1ence did not inf1uence ce11 cyc1e and pro1iferation of ga11b1adder ce11 GBC-SD,but can significant1y inhibit GBC-SD ce11 invasion and movement abi1ity,CCR4 gene si1ence had no inf1uence on the expression of CCL17 and CCL22 gene in tumor ce11s. Conclusion Ga11b1adder carcinoma ce11s GBC-SD express chemokine receptor CCR4,chemokine receptor CCR4 can promote the invasion and metastasis of GBC-SD ce11s.