CAJ | 학술논문

目的：观察胰岛素样生长因子（ insu1in-1ike growth factor，IGF）-Ⅱ及胰岛素样生长因子结合蛋白（ insu1in-1ike growth factor binding protein，IGFBP）-6在结直肠癌（ co1orecta1 cancer，CRC）组织中的表达及临床意义。方法采用免疫组化MaxVision法及RT-PCR法检测50例CRC组织和50例对应癌旁正常结直肠黏膜组织中IGF-Ⅱ及IGFBP-6的表达。结果（1）CRC组织中IGF-Ⅱ蛋白及mRNA表达量明显高于癌旁正常结直肠黏膜组织；CRC组织中IGFBP-6蛋白及mRNA表达量均明显低于癌旁正常结直肠黏膜组织；（2）IGF-Ⅱ和IGFBP-6表达与肿瘤浸润深度、有无淋巴结转移及Dukes分期有关（P<0.05）；IGF-Ⅱ及IGFBP-6表达与患者性别、年龄、肿瘤分化程度无关（ P>0.05）。结论 IGF-Ⅱ及IGFBP-6的蛋白表达及mRNA表达量与CRC临床病理特征有明显相关性。联合检测IGF-Ⅱ及IGFBP-6有可能作为CRC发生及预后的生物学指标，为CRC的诊断及治疗提供新的方案。
목적：관찰이도소양생장인자（ insu1in-1ike growth factor，IGF）-Ⅱ급이도소양생장인자결합단백（ insu1in-1ike growth factor binding protein，IGFBP）-6재결직장암（ co1orecta1 cancer，CRC）조직중적표체급림상의의。방법채용면역조화MaxVision법급RT-PCR법검측50례CRC조직화50례대응암방정상결직장점막조직중IGF-Ⅱ급IGFBP-6적표체。결과（1）CRC조직중IGF-Ⅱ단백급mRNA표체량명현고우암방정상결직장점막조직；CRC조직중IGFBP-6단백급mRNA표체량균명현저우암방정상결직장점막조직；（2）IGF-Ⅱ화IGFBP-6표체여종류침윤심도、유무림파결전이급Dukes분기유관（P<0.05）；IGF-Ⅱ급IGFBP-6표체여환자성별、년령、종류분화정도무관（ P>0.05）。결론 IGF-Ⅱ급IGFBP-6적단백표체급mRNA표체량여CRC림상병리특정유명현상관성。연합검측IGF-Ⅱ급IGFBP-6유가능작위CRC발생급예후적생물학지표，위CRC적진단급치료제공신적방안。
Purpose To investigate the expression of IGF-Ⅱand IGFBP-6 in co1orecta1 cancer,and to exp1ore the c1inica1 significance in co1orecta1 cancer. Methods IGF-Ⅱand IGFBP-6 were detected by immunohistochemistry and RT-PCR in 50 cases of co1orecta1 cancer(experimenta1 group)and 50 cases of the adjacent mucosa(contro1 group). Results (1)In the experimenta1 group,the ex-pression 1eve1 of IGF-Ⅱprotein and mRNA was significant1y higher than the contro1 group. The expression 1eve1s of IGF-Ⅱprotein and mRNA in co1orecta1 cancer were significant1y 1ower than the contro1 group.(2)The expression 1eve1s of IGF-Ⅱand IGFBP-6 were sig-nificant1y different between different tumor infi1tration depth,1ymph node metastasis,invasion depth and Duke’s stages( P<0. 05), but no difference between genders,age and the degree of tumor differentiation( P>0. 05). Conclusions There is a obvious corre1a-tion between of IGF-Ⅱ and IGFBP-6 in c1inica1 patho1ogica1 parameters in co1orecta1 cancer. Combined detection of the two markers may be the bio1ogica1 indicators of occurrence and prognosis of co1orecta1 cancer,and provide a new scheme for diagnosis and treatment of co1orecta1 cancer.