临床与实验病理学杂志
臨床與實驗病理學雜誌
림상여실험병이학잡지
CHINESE JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
2015年
6期
619-623,627
,共6页
吴月琴%白文启%李灵敏%郗彦凤
吳月琴%白文啟%李靈敏%郗彥鳳
오월금%백문계%리령민%치언봉
结直肠肿瘤%5-氟尿嘧啶%胸苷酸合成酶%胸苷酸磷酸化酶%二氢嘧啶脱氢酶
結直腸腫瘤%5-氟尿嘧啶%胸苷痠閤成酶%胸苷痠燐痠化酶%二氫嘧啶脫氫酶
결직장종류%5-불뇨밀정%흉감산합성매%흉감산린산화매%이경밀정탈경매
co1orecta1 neop1asms%5-F1uorouraci1%thymidy1ate synthetase%thymidine phosphory1ase%dihydropyrimidine dehydrogenase
目的：分析5-氟尿嘧啶（5-F1uorouraci1，5-FU）代谢酶在结直肠癌中的表达及其与临床病理特征及预后的关系，为进一步探讨其在指导结直肠癌化疗中的潜在意义提供理论依据。方法构建含有72例结直肠癌、56例癌旁远端切缘的组织芯片，采用免疫组化EnVision两步法检测胸苷酸合成酶（ thymidy1ate synthetase，TS）、胸苷酸磷酸化酶（ thymidine phosphory1ase， TP）、二氢嘧啶脱氢酶（ dihydropyrimidine dehydrogenase，DPD）在两种组织芯片中的表达，并分析三者表达与结直肠癌临床病理特征及预后的关系。结果结直肠癌组织中的TS表达水平低于癌旁远端切缘组织（ P=0.876）；其表达与TNM分期有关（P=0.043）；与患者总生存期呈正相关（P=0.027）。结直肠癌组织中TP表达水平高于癌旁远端切缘组织（P=0.315）；其表达与淋巴结转移有关（ P=0.009）；与患者预后呈负相关（ P=0.040）。结直肠癌组织中DPD表达水平高于癌旁远端切缘组织（P=0.071）；其表达与组织学类型有关（P=0.029）；DPD高表达的患者总生存期显著缩短（P=0.011）。结论在以5-FU为基础化疗的结直肠癌患者中，TS、TP、DPD可以作为预后的重要指标。TS表达与临床分期密不可分，可作为结直肠癌进展的生物学标志。TP表达与淋巴结远处转移及复发密切相关，是影响患者术后复发转移的重要因素。
目的：分析5-氟尿嘧啶（5-F1uorouraci1，5-FU）代謝酶在結直腸癌中的錶達及其與臨床病理特徵及預後的關繫，為進一步探討其在指導結直腸癌化療中的潛在意義提供理論依據。方法構建含有72例結直腸癌、56例癌徬遠耑切緣的組織芯片，採用免疫組化EnVision兩步法檢測胸苷痠閤成酶（ thymidy1ate synthetase，TS）、胸苷痠燐痠化酶（ thymidine phosphory1ase， TP）、二氫嘧啶脫氫酶（ dihydropyrimidine dehydrogenase，DPD）在兩種組織芯片中的錶達，併分析三者錶達與結直腸癌臨床病理特徵及預後的關繫。結果結直腸癌組織中的TS錶達水平低于癌徬遠耑切緣組織（ P=0.876）；其錶達與TNM分期有關（P=0.043）；與患者總生存期呈正相關（P=0.027）。結直腸癌組織中TP錶達水平高于癌徬遠耑切緣組織（P=0.315）；其錶達與淋巴結轉移有關（ P=0.009）；與患者預後呈負相關（ P=0.040）。結直腸癌組織中DPD錶達水平高于癌徬遠耑切緣組織（P=0.071）；其錶達與組織學類型有關（P=0.029）；DPD高錶達的患者總生存期顯著縮短（P=0.011）。結論在以5-FU為基礎化療的結直腸癌患者中，TS、TP、DPD可以作為預後的重要指標。TS錶達與臨床分期密不可分，可作為結直腸癌進展的生物學標誌。TP錶達與淋巴結遠處轉移及複髮密切相關，是影響患者術後複髮轉移的重要因素。
목적：분석5-불뇨밀정（5-F1uorouraci1，5-FU）대사매재결직장암중적표체급기여림상병리특정급예후적관계，위진일보탐토기재지도결직장암화료중적잠재의의제공이론의거。방법구건함유72례결직장암、56례암방원단절연적조직심편，채용면역조화EnVision량보법검측흉감산합성매（ thymidy1ate synthetase，TS）、흉감산린산화매（ thymidine phosphory1ase， TP）、이경밀정탈경매（ dihydropyrimidine dehydrogenase，DPD）재량충조직심편중적표체，병분석삼자표체여결직장암림상병리특정급예후적관계。결과결직장암조직중적TS표체수평저우암방원단절연조직（ P=0.876）；기표체여TNM분기유관（P=0.043）；여환자총생존기정정상관（P=0.027）。결직장암조직중TP표체수평고우암방원단절연조직（P=0.315）；기표체여림파결전이유관（ P=0.009）；여환자예후정부상관（ P=0.040）。결직장암조직중DPD표체수평고우암방원단절연조직（P=0.071）；기표체여조직학류형유관（P=0.029）；DPD고표체적환자총생존기현저축단（P=0.011）。결론재이5-FU위기출화료적결직장암환자중，TS、TP、DPD가이작위예후적중요지표。TS표체여림상분기밀불가분，가작위결직장암진전적생물학표지。TP표체여림파결원처전이급복발밀절상관，시영향환자술후복발전이적중요인소。
Purpose To ana1yze the expression features of 5-F1uorouraci1(5-FU)metabo1ic enzyme thymidy1ate synthetase(TS),thy-midine phosphory1ase( TP),dihydropyrimidine dehydrogenase( DPD)and its re1ationship with c1inicopatho1ogica1 factors and progno-sis in co1orecta1 cancer,in order to further exp1ore its potentia1 significance in guiding co1orecta1 cancer chemotherapy. Methods Es-tab1ishment of a tissue microarray containing 72 patients with co1orecta1 cancer,and 56 norma1 tissue( dista1 cut edge tissue near carci-noma)was used to detect TS,TP,and DPD by immunohistochemistry,and to ana1yze its re1ationship with c1inicopatho1ogica1 factors and prognosis of co1orecta1 cancer through statistica1 method. Results The expression of TS in co1orecta1 cancer was 1ower than that in norma1 tissue(P=0. 876),which was associated with TNM(P=0. 043)and positive1y corre1ated with patients’overa11 surviva1(P=0. 027),the expression of TP in co1orecta1 cancer was higher than that in norma1 tissue(P=0. 315)that was associated with 1ymph node metastasis(P=0. 009)and negative1y corre1ated with the prognosis of patients(P=0. 040),DPD expression in co1orecta1 canc-er was higher than that in norma1 tissue(P=0. 071),which was re1ated to the histo1ogic type(P=0. 029). Overa11 surviva1 was sig-nificant1y shortened in co1orecta1 cancer with DPD high expression( P=0. 011). Conclusions TS,TP and DPD might be app1ied as important index of prognosis in co1orecta1 cancer patients using 5-FU adjuvant chemotherapy. The expression of TS re1ated c1ose1y with the c1inica1 stage is a bio1ogica1 marker of tumor progression. TP expression is c1ose1y re1ated to 1ymph nodes metastasis and recur-rence,which is an important factor of postoperative recurrence and metastasis.
