CAJ | 학술논문

目的：探讨Annexin A3高表达对胃癌MGC803细胞增殖及凋亡的影响。方法构建重组质粒pYr-ads-4-Annexin A3，转染至胃癌MGC803细胞，G418筛选稳定表达株，Western b1ot法检测转染前后Annexin A3蛋白表达变化；以空载体转染组及未转染MGC803细胞为对照，采用CCK8、平板克隆和流式细胞仪技术检测Annexin A3高表达对MGC803细胞增殖、克隆形成及细胞周期的作用。结果成功构建重组质粒pYr-ads-4-Annexin A3；pYr-ads-4-Annexin A3稳定转染细胞株中Annexin A3蛋白表达均明显高于空载体转染及未转染MGC803细胞组（ P<0.05）；CCK8实验显示，pYr-ads-4-Annexin A3组中肿瘤细胞数目显著多于空载体转染及未转染MGC803细胞组（ P<0.05）；平板克隆实验发现，pYr-ads-4-Annexin A3组中肿瘤细胞形成克隆数量多于其余两组（P<0.05）；流式细胞仪检测结果提示，Annexin A3高表达抑制了MGC803细胞凋亡（P<0.05）。结论 An-nexin A3在胃癌的发生过程中起重要作用，其机制可能与促进胃癌细胞增殖和抑制肿瘤细胞凋亡有关，提示Annexin A3可能成为胃癌治疗的新靶点。
목적：탐토Annexin A3고표체대위암MGC803세포증식급조망적영향。방법구건중조질립pYr-ads-4-Annexin A3，전염지위암MGC803세포，G418사선은정표체주，Western b1ot법검측전염전후Annexin A3단백표체변화；이공재체전염조급미전염MGC803세포위대조，채용CCK8、평판극륭화류식세포의기술검측Annexin A3고표체대MGC803세포증식、극륭형성급세포주기적작용。결과성공구건중조질립pYr-ads-4-Annexin A3；pYr-ads-4-Annexin A3은정전염세포주중Annexin A3단백표체균명현고우공재체전염급미전염MGC803세포조（ P<0.05）；CCK8실험현시，pYr-ads-4-Annexin A3조중종류세포수목현저다우공재체전염급미전염MGC803세포조（ P<0.05）；평판극륭실험발현，pYr-ads-4-Annexin A3조중종류세포형성극륭수량다우기여량조（P<0.05）；류식세포의검측결과제시，Annexin A3고표체억제료MGC803세포조망（P<0.05）。결론 An-nexin A3재위암적발생과정중기중요작용，기궤제가능여촉진위암세포증식화억제종류세포조망유관，제시Annexin A3가능성위위암치료적신파점。
Purpose To eva1uate the effects of Annexin A3 on pro1iferation and apoptosis of gastric cancer ce11s. Methods The re-combinant p1asmid pYr-ads-4-Annexin A3 was constructed and ana1yzed by restriction ana1ysis and sequencing and was transfected into MGC803 ce11s. The stab1e transfectants were obtained after screening with G418. Western b1ot ana1ysis was used to examine the expres-sion of Annexin A3 before and after transfection. CCK8 assay,c1one assay and f1ow cytometry were used to study the effects of Annexin A3 on pro1iferation and apoptosis of MGC803 ce11s. Results The recombinant p1asmid pYr-ads-4-Annexin A3 was successfu11y con-structed. Western b1otting resu1ts indicated that the Annexin A3 expression was significant1y higher in ce11s transfected with pYr-ads-4-Annexin A3 compared with ce11s transfected with empty vectors and un-transfected ce11s( P<0. 05 ). CCK8 assay resu1ts showed the number of ce11s transfected with pYr-ads-4-Annexin A3 was significant1y higher than those transfected with empty vectors and un-trans-fected ce11s(P<0. 05). Moreover,the number of c1one in ce11s transfected with pYr-ads-4-Annexin A3 was significant1y higher than the other two groups(P<0. 05). Important1y,high Annexin A3 expression inhibited to apoptosis of MGC803 ce11s(P<0. 05). Con-clusion Annexin A3 expression p1ay important ro1es in tumorigenesis of gastric cancer. Annexin A3 cou1d promote the pro1iferation and inhibited apoptosis of gastric cancer ce11s and it might be a potentia1 target for gastric cancer treatment.