CAJ | 학술논문

目的：研究红芪多糖（HPS）对人牙周膜细胞（hPDLCs）增殖及分泌IL－1β、IL－6的影响。方法：采用改良组织块酶消化法培养人牙周膜细胞，经免疫组化SP法进行鉴定，MTT法检测细胞增殖情况，荧光实时定量PCR和酶联免疫法检测红芪多糖对LPS作用的hPDLCs分泌IL－1β、IL－6的影响。结果：10μg／mL HPS能够促进人牙周膜细胞增殖（P＜0．05）；10μg／mL LPS可显著刺激人牙周膜细胞分泌IL－1β、IL－6（P＜0．01），而给予10μg／mL HPS能抑制LPS刺激人牙周膜细胞分泌IL－1β、IL－6，且与LPS组具有显著性差异（P＜0．01）。结论：适宜浓度的红芪多糖能够促进体外培养的人牙周膜细胞增殖，并抑制LPS作用的人牙周膜细胞分泌IL－1β、IL－6。
목적：연구홍기다당（HPS）대인아주막세포（hPDLCs）증식급분비IL－1β、IL－6적영향。방법：채용개량조직괴매소화법배양인아주막세포，경면역조화SP법진행감정，MTT법검측세포증식정황，형광실시정량PCR화매련면역법검측홍기다당대LPS작용적hPDLCs분비IL－1β、IL－6적영향。결과：10μg／mL HPS능구촉진인아주막세포증식（P＜0．05）；10μg／mL LPS가현저자격인아주막세포분비IL－1β、IL－6（P＜0．01），이급여10μg／mL HPS능억제LPS자격인아주막세포분비IL－1β、IL－6，차여LPS조구유현저성차이（P＜0．01）。결론：괄의농도적홍기다당능구촉진체외배양적인아주막세포증식，병억제LPS작용적인아주막세포분비IL－1β、IL－6。
Objective:The purpose of this study is to observe the effect of radix hedysari polysaccharide(HPS) on the proliferation and expression of interleukin(IL)-1β,IL-6 in human periodontal ligament cells(hPDLCs) and to investigate the effect of HPS on the periodontal inflammatory reaction. Method:Primary hPDLCs were cultured by tissue block enzymolytic method,cells were used after immunohistochemical identification(with SP method) in this experiment. 3-(4,5-Dimethylthi-azol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay were used to examine the effect of HPS on the proliferation of hPDLCs. The effect of HPS on the expression of IL-1β,IL-6 in hPDLCs treated with LPS were determined by quantitative real-time polymerase chain reaction(qRT-PCR) and enzyme-linked immunosorbent assay(ELISA). Result:HPS at a con-centration of 10 μg/mL promoted hPDLCs proliferation.The expression of IL-1β,IL-6 was significantly increased with E. Coli LPS at 10μg/mL(P<0.01). After adding HPS at 10 μg/mL,the production of IL-1β,IL-6 was inhibited(P<0.05). Conclusion:Radix hedysari polysaccharide could stimulated the cell proliferation in vitro,inhibit the expression of IL-1β, IL-6 in human periodontal ligament cells treated with LPS. As a traditional Chinese herb,its mechanism of action of peri-odontal inflammation still needs further study.