中国药品标准
中國藥品標準
중국약품표준
DRUG STANDARDS OF CHINA
2015年
3期
176-180
,共5页
余芬%孙明波%沈伟%杨兴鹅%杨普珊%伍胤杰%李佳%杨希
餘芬%孫明波%瀋偉%楊興鵝%楊普珊%伍胤傑%李佳%楊希
여분%손명파%침위%양흥아%양보산%오윤걸%리가%양희
培养基%高压灭菌%pH最适范围
培養基%高壓滅菌%pH最適範圍
배양기%고압멸균%pH최괄범위
media%autoclaving%pH optimum range
目的：通过检测、分析改良马丁培养基等6种生物制品生产中常用液体培养基高压灭菌前后的pH变化，给实际配制培养基时预调pH的范围提供参考。方法：将培养基干粉按要求配制，用0.5 mol · L－1 NaOH溶液调节成不同的pH梯度（6.80～8.10），经高压灭菌后检测pH值，并对结果进行对比、分析、统计。结果：改良马丁培养基、硫乙醇酸盐流体培养基、麦康凯肉汤培养基、胰酪胨大豆肉汤培养基4种灭菌后pH降低，其中，改良马丁培养基的下降幅度最大；营养肉汤培养基灭菌后pH升高；精氨酸支原体肉汤培养基变化不显著。结论：不同培养基灭菌前后的pH变化差异较大，根据pH检测结果变化规律得出各培养基高压灭菌前最适的pH预调范围。
目的：通過檢測、分析改良馬丁培養基等6種生物製品生產中常用液體培養基高壓滅菌前後的pH變化，給實際配製培養基時預調pH的範圍提供參攷。方法：將培養基榦粉按要求配製，用0.5 mol · L－1 NaOH溶液調節成不同的pH梯度（6.80～8.10），經高壓滅菌後檢測pH值，併對結果進行對比、分析、統計。結果：改良馬丁培養基、硫乙醇痠鹽流體培養基、麥康凱肉湯培養基、胰酪胨大豆肉湯培養基4種滅菌後pH降低，其中，改良馬丁培養基的下降幅度最大；營養肉湯培養基滅菌後pH升高；精氨痠支原體肉湯培養基變化不顯著。結論：不同培養基滅菌前後的pH變化差異較大，根據pH檢測結果變化規律得齣各培養基高壓滅菌前最適的pH預調範圍。
목적：통과검측、분석개량마정배양기등6충생물제품생산중상용액체배양기고압멸균전후적pH변화，급실제배제배양기시예조pH적범위제공삼고。방법：장배양기간분안요구배제，용0.5 mol · L－1 NaOH용액조절성불동적pH제도（6.80～8.10），경고압멸균후검측pH치，병대결과진행대비、분석、통계。결과：개량마정배양기、류을순산염류체배양기、맥강개육탕배양기、이락동대두육탕배양기4충멸균후pH강저，기중，개량마정배양기적하강폭도최대；영양육탕배양기멸균후pH승고；정안산지원체육탕배양기변화불현저。결론：불동배양기멸균전후적pH변화차이교대，근거pH검측결과변화규률득출각배양기고압멸균전최괄적pH예조범위。
Objective:By detecting the pH value of six media before and after autoclaving to set up a certain pH reference in media prep-aration.Methods:The pH value of media were adjusted to 6.80-8.10 with 0.5 mol· L-1 NaOH before autoclaving and detected after autoclaving, then the characteristics of pH variations were compared and analyzed.Results: The pH value of four media, which were Martin Medium Modified, Thioglycollate Medium, MacConkey Broth Medium and Tryptone Soy Broth Medium (TSB), decreased after autoclaving.There was significant reduction in pH for Martin Medium Modified.Nutrient Broth Medium′s pH increased after autoclaving.Mycoplasma ( Ma) Broth Medium Base′s pH was hardly changed.Conclusion:The variations of pH of six media after autoclaving is dif-ferent.This paper provided the appropriate range of pH pre-adjustment in media preparation.
