CAJ | 학술논문

建立高效液相色谱法分离测定“黑美人”马铃薯中原花青素含量的分析方法。实验采用C18柱（Phenomenex 5μm；250 mm ×4.6 mm）；V（甲醇）∶V（0.5%冰醋酸水溶液）=30∶70为流动相；柱温：25℃；流速：1 mL/min；检测波长：287 nm。结果表明：原花青素在80μg/mL~200μg/mL浓度范围内有良好的线性关系，相关系数R=0.9957，方法的精密度RSD为2.86%，平均回收率为100.1%，原花青素的平均含量为51.805 mg/g。本方法简便、快速、准确、可靠，可用于“黑美人”马铃薯中原花青素的含量的测定。
건립고효액상색보법분리측정“흑미인”마령서중원화청소함량적분석방법。실험채용C18주（Phenomenex 5μm；250 mm ×4.6 mm）；V（갑순）∶V（0.5%빙작산수용액）=30∶70위류동상；주온：25℃；류속：1 mL/min；검측파장：287 nm。결과표명：원화청소재80μg/mL~200μg/mL농도범위내유량호적선성관계，상관계수R=0.9957，방법적정밀도RSD위2.86%，평균회수솔위100.1%，원화청소적평균함량위51.805 mg/g。본방법간편、쾌속、준학、가고，가용우“흑미인”마령서중원화청소적함량적측정。
Proanthocyanidins in “Heimeiren”Potatoes was determined by HPLC. Phenomenex C18 column (5μm;250 mm×4.6 mm) was used with the mobile phase of 30%methanol and 70 %water(with 0.5%acetic acid).The column temperature, flow rate and detection wavelength were 25 ℃, 1 mL/min and 287 nm, respectively. The results showed that there was a good linear relationship between peak area and proanthocyanidins content within the range of 80 μg/mL-200 μg/mL. Linear correlation was 0.995 7,RSD of precision was 2.86%,the average recovery rate was 100.1 %and the average content of proanthocyanidins was 51.805 mg/g. This method is simple, fast, accurate and reliable, suitable for determination of proanthocyanidins content in“Heimeiren”Potatoes.