CAJ | 학술논문

研究了Seveage法对紫苏叶多糖的脱蛋白工艺。结果表明，氯仿与正丁醇的体积比为4∶1，样液与Seveage试剂的体积比为1∶1，时间为30 min，次数为4次，紫苏叶多糖的脱蛋白率为72.1%，多糖损失率为17.3%。紫苏叶多糖提取液经活性炭脱色、Seveage脱蛋白后通过DEAE-纤维素柱层析，采用蒸馏水、0.2、0.4、0.6、0.8、1.0 mol/L NaCl洗脱液进行洗脱，分离得到紫苏叶多糖Ⅰ（PLPⅠ）3.18μg/mL、紫苏叶多糖Ⅱ（PLPⅡ）4.64μg/mL、紫苏叶多糖Ⅲ（PLPⅢ）12.22μg/mL、紫苏叶多糖Ⅳ（PLPⅣ）26.36μg/mL、紫苏叶多糖Ⅴ（PLPⅤ）32.63μg/mL、紫苏叶多糖Ⅵ（PLPⅥ）62.52μg/mL六个紫苏叶多糖组分。
연구료Seveage법대자소협다당적탈단백공예。결과표명，록방여정정순적체적비위4∶1，양액여Seveage시제적체적비위1∶1，시간위30 min，차수위4차，자소협다당적탈단백솔위72.1%，다당손실솔위17.3%。자소협다당제취액경활성탄탈색、Seveage탈단백후통과DEAE-섬유소주층석，채용증류수、0.2、0.4、0.6、0.8、1.0 mol/L NaCl세탈액진행세탈，분리득도자소협다당Ⅰ（PLPⅠ）3.18μg/mL、자소협다당Ⅱ（PLPⅡ）4.64μg/mL、자소협다당Ⅲ（PLPⅢ）12.22μg/mL、자소협다당Ⅳ（PLPⅣ）26.36μg/mL、자소협다당Ⅴ（PLPⅤ）32.63μg/mL、자소협다당Ⅵ（PLPⅥ）62.52μg/mL륙개자소협다당조분。
The conditions of deproteinization from perilla leaf polysaccharide were studied by using Seveage method. The result showed that the best operation condition was that, chloroform and1-butanol ratio of 4, sample liquid and Sevage liquid ratio of 1, protein removal time of 30 minutes and protein removal cycle numbers of 4, which the deproteinization rate was 72.1%and the loss ratio of polysaccharide was 17.3%. After decolor by activated charcoal and deproteinizate by Seveage , the extracting solution of perilla leaf polysaccharide could be separated by DEAE-cellulose column chromatography , and gradiantly eluted with distilled water, 0.2, 0.4, 0.6, 0.8, 1.0 mol/L NaCl eluant to all can get six components named PLPⅠ3.18μg/mL, PLPⅡ4.64μg/mL, PLPⅢ12.22μg/mL, PLPⅣ26.36μg/mL, PLPⅤ32.63μg/mL, PLPⅥ62.52μg/mL.