CAJ | 학술논문

目的：探究巨噬细胞移动抑制因子（MIF）在兔心肌缺血/再灌注及急性心肌梗死模型中的动态变化。方法实验动物为雄性新西兰大白兔18只，随机分为假手术组、心肌梗死组及再灌注组，每组各6只。以“二线二结法”构造再灌注及心梗模型，假手术组仅穿线不结扎。心肌梗死组一直结扎左前降支；再灌注组均给以结扎30 min再灌注60 min处理。分别在术前1h、术后3 h、6 h、24 h、48 h、72 h股静脉穿刺抽血，酶联免疫吸附法检测血浆MIF浓度。结果三组术前MIF浓度无统计学差异（P均＞0.05）。与假手术组比较，再灌注组以及心肌梗死组术后3 h、6 h、24 h、48 h、72 h MIF浓度均升高，差异有统计学意义（P均＜0.05）。结论急性心肌梗死及缺血/再灌注过程中，MIF释放增加，浓度升高。
목적：탐구거서세포이동억제인자（MIF）재토심기결혈/재관주급급성심기경사모형중적동태변화。방법실험동물위웅성신서란대백토18지，수궤분위가수술조、심기경사조급재관주조，매조각6지。이“이선이결법”구조재관주급심경모형，가수술조부천선불결찰。심기경사조일직결찰좌전강지；재관주조균급이결찰30 min재관주60 min처리。분별재술전1h、술후3 h、6 h、24 h、48 h、72 h고정맥천자추혈，매련면역흡부법검측혈장MIF농도。결과삼조술전MIF농도무통계학차이（P균＞0.05）。여가수술조비교，재관주조이급심기경사조술후3 h、6 h、24 h、48 h、72 h MIF농도균승고，차이유통계학의의（P균＜0.05）。결론급성심기경사급결혈/재관주과정중，MIF석방증가，농도승고。
Objective To study the dynamic changes of macrophage migration inhibitory factor (MIF) in myocardial ischemia/reperfusion and acute myocardial infarction (AMI) in rabbits. Methods Male New Zealand rabbits (n=18) were randomly divided into sham-operation group, AMI group and reperfusion group (each n=6). The models of reperfusion and AMI were established by using 2-suture 2-ligation method, sham-operation group was not given ligation, AMI group was given ligation of left anterior descending branch, and reperfusion group was given ligation for 30 min and reperfusion for 60 min. The blood samples were collected from femoral vein at different time points including 1 h before operation and 3 h, 6 h, 24 h, 48 h and 72 h after operation for detecting plasma MIF concentration by using enzyme-linked immunosorbent assay (ELISA). Results The plasma MIF concentration had no statistical difference among 3 groups before operation (all P>0.05), and increased in reperfusion group and AMI group 3 h, 6 h, 24 h, 48 h and 72 h after operation compared with sham-operation group (all P<0.05). Conclusion MIF will release more and its concentration will increase during the process of AMI and ischemia/reperfusion.