中国循环杂志
中國循環雜誌
중국순배잡지
CHINESE CIRCULATION JOURNAL
2015年
6期
576-579
,共4页
盛冲霄%黎红华%刘康%冯海松
盛遲霄%黎紅華%劉康%馮海鬆
성충소%려홍화%류강%풍해송
动脉粥样硬化%亲环素A%内皮依赖性舒张功能
動脈粥樣硬化%親環素A%內皮依賴性舒張功能
동맥죽양경화%친배소A%내피의뢰성서장공능
Atherosclerosis%Cyclophilin A%Endothelial-dependent vasodilatation
目的：观察高脂饮食喂养大鼠动脉粥样硬化病变各阶段内皮依赖性舒张功能的改变以及亲环素A（CyPA）的表达变化。方法：雄性Wistar大鼠30只随机分为4组，对照组（6只），动脉粥样硬化8周组（AS 8W组）8只，动脉粥样硬化12周组（AS 12W组）8只，动脉粥样硬化15周组（AS 15W组）8只。给予动脉粥样硬化组高脂饲料喂养，并给予维生素D3注射液60万IU/kg一次性腹腔注射，对照组给予基础饲料喂养。同期处死大鼠，分离腹主动脉，行苏木素伊红（HE）染色，免疫组化法检测动脉壁CyPA的表达。另外截取约5 mm新鲜大鼠腹主动脉环，在离体器官浴槽中观察离体腹主动脉环对乙酰胆碱舒张反应。结果：随着造模时间的进展，各组大鼠分别呈现出了血管正常结构、内皮细胞破坏，平滑肌细胞增生、粥样斑块形成以及钙化斑块形成等不同的病变特征。内皮依赖性舒张功能进行性下降，对照组、AS 8W组、AS 12W组、AS 15W组最大舒张度分别为（93.46±2.80）%、（82.58±3.25）%、（61.19±3.72）%、（41.28±2.68）%，各组间差异均有统计学意义（P均＜0.05）。血管内皮细胞以及平滑肌细胞CyPA的表达不断增加，四组大鼠CyPA平均光密度测定分别为0.25±0.06、0.34±0.09、0.53±0.09、0.68±0.13，各组间比较差异均有统计学意义（P均＜0.05）。结论： CyPA的表达随着病变进展不断增加，内皮依赖血管舒张功能进行性下降。CyPA的表达与动脉粥样硬化病变严重程度有关。CyPA可能是动脉粥样硬化的始动因素之一。
目的：觀察高脂飲食餵養大鼠動脈粥樣硬化病變各階段內皮依賴性舒張功能的改變以及親環素A（CyPA）的錶達變化。方法：雄性Wistar大鼠30隻隨機分為4組，對照組（6隻），動脈粥樣硬化8週組（AS 8W組）8隻，動脈粥樣硬化12週組（AS 12W組）8隻，動脈粥樣硬化15週組（AS 15W組）8隻。給予動脈粥樣硬化組高脂飼料餵養，併給予維生素D3註射液60萬IU/kg一次性腹腔註射，對照組給予基礎飼料餵養。同期處死大鼠，分離腹主動脈，行囌木素伊紅（HE）染色，免疫組化法檢測動脈壁CyPA的錶達。另外截取約5 mm新鮮大鼠腹主動脈環，在離體器官浴槽中觀察離體腹主動脈環對乙酰膽堿舒張反應。結果：隨著造模時間的進展，各組大鼠分彆呈現齣瞭血管正常結構、內皮細胞破壞，平滑肌細胞增生、粥樣斑塊形成以及鈣化斑塊形成等不同的病變特徵。內皮依賴性舒張功能進行性下降，對照組、AS 8W組、AS 12W組、AS 15W組最大舒張度分彆為（93.46±2.80）%、（82.58±3.25）%、（61.19±3.72）%、（41.28±2.68）%，各組間差異均有統計學意義（P均＜0.05）。血管內皮細胞以及平滑肌細胞CyPA的錶達不斷增加，四組大鼠CyPA平均光密度測定分彆為0.25±0.06、0.34±0.09、0.53±0.09、0.68±0.13，各組間比較差異均有統計學意義（P均＜0.05）。結論： CyPA的錶達隨著病變進展不斷增加，內皮依賴血管舒張功能進行性下降。CyPA的錶達與動脈粥樣硬化病變嚴重程度有關。CyPA可能是動脈粥樣硬化的始動因素之一。
목적：관찰고지음식위양대서동맥죽양경화병변각계단내피의뢰성서장공능적개변이급친배소A（CyPA）적표체변화。방법：웅성Wistar대서30지수궤분위4조，대조조（6지），동맥죽양경화8주조（AS 8W조）8지，동맥죽양경화12주조（AS 12W조）8지，동맥죽양경화15주조（AS 15W조）8지。급여동맥죽양경화조고지사료위양，병급여유생소D3주사액60만IU/kg일차성복강주사，대조조급여기출사료위양。동기처사대서，분리복주동맥，행소목소이홍（HE）염색，면역조화법검측동맥벽CyPA적표체。령외절취약5 mm신선대서복주동맥배，재리체기관욕조중관찰리체복주동맥배대을선담감서장반응。결과：수착조모시간적진전，각조대서분별정현출료혈관정상결구、내피세포파배，평활기세포증생、죽양반괴형성이급개화반괴형성등불동적병변특정。내피의뢰성서장공능진행성하강，대조조、AS 8W조、AS 12W조、AS 15W조최대서장도분별위（93.46±2.80）%、（82.58±3.25）%、（61.19±3.72）%、（41.28±2.68）%，각조간차이균유통계학의의（P균＜0.05）。혈관내피세포이급평활기세포CyPA적표체불단증가，사조대서CyPA평균광밀도측정분별위0.25±0.06、0.34±0.09、0.53±0.09、0.68±0.13，각조간비교차이균유통계학의의（P균＜0.05）。결론： CyPA적표체수착병변진전불단증가，내피의뢰혈관서장공능진행성하강。CyPA적표체여동맥죽양경화병변엄중정도유관。CyPA가능시동맥죽양경화적시동인소지일。
Objective: To observe the changes of endothelial-dependent vasodilatation and cyclophilin A (CyPA) expression at different phases of atherosclerosis in experimental rats. Methods: A total of 30 male Wistar rats were divided into 4 groups: Control group, the rats received normal diet, and 3 atherosclerosis groups, the rats received high cholesterol diet for different period of time and a single dose intraperitoneal injection of Vitamin D3 at 600,000 IU/kg, as Atherosclerosis at 8 weeks (AS 8W) group, AS 12W group and AS 15W group, n=8 in each AS group. The rats were sacriifced at the same time to isolate aorta abdominalis. The expression of CyPA at the wall of aorta abdominalis was detected by HE staining and immunohistochemistry. In addition, the aorta abdominalises was cut into 5 mm rings to observe its response to acethylcholine in exvivo organ bath. Results: With prolonged modeling time, the rats at different groups presented normal vessel structure, endothelial cell damage, vessel smooth muscle cell proliferation, atherosclerosis plaque formation and calciifed plaque formation for differentpathological characteristics. The endothelial-dependent vasodilatation was decreased and the maximum vasodilatation percentage in Control group, AS 8W group, AS 12W group and AS 15W group were at (93.46 ± 2.80) %, (82.58 ± 3.25) %, (61.19 ± 3.72)% and (41.28 ± 2.68)% respectively,P<0.05 between each group. The CyPA expression in endothelial cell and vessel smooth muscle cell were increased accordingly in 4 groups by OD value as (0.25 ± 0.06), (0.34 ± 0.09), (0.53 ± 0.09) and (0.68 ± 0.13) respectively,P<0.05 between each group. Conclusion: The CyPA expression increased and the endothelial-dependent vasodilatation decreased with the progress of atherosclerosis accordingly in experimental rats. The expression level of CyPA is related to atherosclerosis degree and it is one of the initial factors for atherosclerosis in rats.
