CAJ | 학술논문

通过PCR-DGGE和测序等方法分析了铜累积性植物茵陈蒿（Artemisia capillaris Thunb.）根际的细菌群落，并研究了耐铜细菌对纳米CuO（CuO NPs）的淋滤作用，试图提高CuO NPs的生物有效性，以加速修复进程。结果表明，茵陈蒿根际细菌多样性很低，主要由气球菌属、寡养单胞菌属和微球菌属组成，其中气球菌属为优势属。低浓度CuO NPs处理对细菌S31的生长有一定促进作用，但400 mg·L-1的CuO NPs则会对其产生明显抑制；不同浓度下，细菌S31对CuO NPs的淋滤作用有明显差异；驯化不仅明显改善了细菌的生长状况，还进一步增强了CuO NPs的淋滤。通过微生物提高人工纳米材料生物有效性的方法能否实际用于环境修复还有待进一步的试验进行验证。
통과PCR-DGGE화측서등방법분석료동루적성식물인진호（Artemisia capillaris Thunb.）근제적세균군락，병연구료내동세균대납미CuO（CuO NPs）적림려작용，시도제고CuO NPs적생물유효성，이가속수복진정。결과표명，인진호근제세균다양성흔저，주요유기구균속、과양단포균속화미구균속조성，기중기구균속위우세속。저농도CuO NPs처리대세균S31적생장유일정촉진작용，단400 mg·L-1적CuO NPs칙회대기산생명현억제；불동농도하，세균S31대CuO NPs적림려작용유명현차이；순화불부명현개선료세균적생장상황，환진일보증강료CuO NPs적림려。통과미생물제고인공납미재료생물유효성적방법능부실제용우배경수복환유대진일보적시험진행험증。
Behavior, environmental fate and biotoxicity of manufactured nano materials(MNMs)have received extensive attention in recent years. However, it is unclear whether MNMs-contaminated soils could be remediated by combined plant and rhizospheric microbes. In this study, bacterial community structure associated with copper(Cu)accumulating plant Artemisia capillaris Thunb. was investigated using PCR-DGGE and sequencing techniques. Subsequently, Cu tolerant bacteria were screened using selective culture medium containing 500 mg·L-1 CuSO4. The effect of Cu tolerant bacteria and bacterial communities on leaching of nano CuO(CuO NPs)was then studied. Results showed that bacterial community in the rhizosphere had very low diversity, and mainly consisted of genus of A erococcus, Stenotrophomonas and Micrococcus, with Aerococcus being the dominant genus. The growth of bacterium S31 was enhanced by low CuO NPs, but suppressed by 400 mg·L-1 CuO NPs. The Cu tolerant bacterium S31 showed a concentration dependent enhancement on leaching of CuO NPs. Acclima-tion significantly improved the growth of bacterium S31 and increased the leaching of CuO NPs. This study indicates the potential to reme-diate MNMs-contaminated soil by combined plant and microbes.