CAJ | 학술논문

以菲降解菌———鞘氨醇单胞菌（Sphingomonas sp.）GY2B和芘降解菌———假单胞菌（Sphingomonas sp.）GP3A为研究对象，对两株菌进行融合前的抗药性标记筛选，融合后的菌株通过形态学及分子生物学进行分析鉴定，并测定其对菲和芘的降解效果。结果表明，筛选出GY2B的遗传标记为哌拉西林抗性（80μg·mL-1），GP3A的遗传标记为头孢他啶抗性（80μg·mL-1）或红霉素抗性（100~150μg·mL-1）。通过菲和芘的初步降解实验筛选出一株融合菌株F14,通过平板菌落形态、扫描电镜（SEM）及PCR-RFLP分析鉴定F14是不同于亲本的菌株，是GY2B和GP3A的融合子。融合菌株F14既可以降解菲又可以降解芘，对初始浓度为100 mg·L-1的菲和芘的降解率分别为99%（24 h）和18%（10 d），降解能力和降解效果明显高于其亲本。
이비강해균———초안순단포균（Sphingomonas sp.）GY2B화비강해균———가단포균（Sphingomonas sp.）GP3A위연구대상，대량주균진행융합전적항약성표기사선，융합후적균주통과형태학급분자생물학진행분석감정，병측정기대비화비적강해효과。결과표명，사선출GY2B적유전표기위고랍서림항성（80μg·mL-1），GP3A적유전표기위두포타정항성（80μg·mL-1）혹홍매소항성（100~150μg·mL-1）。통과비화비적초보강해실험사선출일주융합균주F14,통과평판균락형태、소묘전경（SEM）급PCR-RFLP분석감정F14시불동우친본적균주，시GY2B화GP3A적융합자。융합균주F14기가이강해비우가이강해비，대초시농도위100 mg·L-1적비화비적강해솔분별위99%（24 h）화18%（10 d），강해능력화강해효과명현고우기친본。
In the present study, a phenethrene-degradation strain(Sphingomonas sp.)GY2B and a pyrene-degradation strain(Pseudomonas sp.)GP3A were used to obtain a fusant capable of degrading PAHs. The antibiotic resistance test was used to screen the genetic markers. Morphology and molecular biology were employed to identify fusants. Results showed that the genetic markers of Sphingomonas sp. GY2B was piperacillin(80μg·mL-1)-resistant and Pseudomonas sp. GP3A was ceftazidime(80μg·mL-1)- or erythromycin(100~150μg·mL-1)-resistant, respectively. A fusant strain with the highest degradation of both phenanthrene and pyrene was selected and named F14. Tests by colony morphology, electron microscope, scanning electron microscope(SEM)and molecular technology(PCR-RFLP)indicated that the strain F14 was a fusant of GP3A and GY2B, and was different from its parents. F14 could degrade both phenanthrene and pyrene. It almost completely degraded phenanthrene(100 μg·mL-1)within 24 hours, much quicker than GY2B and other strains. F14 degraded 18% of pyrene(100 mg·L-1)within 10 days, still higher than GP3A.