CAJ | 학술논문

目的：正确认识免疫透射比浊法应用于全自动生化分析仪上的抗原过量产生的钩状效应及对这种钩状效应的防范。方法首先对仪器的测量线性范围上限和仪器自动稀释重做功能进行设置，然后对设置前超过测量范围上限的样本进行设置测定及设置前且人工对倍稀释测定。结果设置前测定组平均值为2．49g／L设置后测定组平均值为3．31g／L设置前且人工对倍稀释测定组平均值为3．33 g／L。结论高剂量钩状效应使强阳性标本误测为弱阳性，甚至假阴性结果，高浓度标本误测为低值，所以对仪器进行设置且对标本倍比稀释来进行测定，测出来的值更接近真值。
목적：정학인식면역투사비탁법응용우전자동생화분석의상적항원과양산생적구상효응급대저충구상효응적방범。방법수선대의기적측량선성범위상한화의기자동희석중주공능진행설치，연후대설치전초과측량범위상한적양본진행설치측정급설치전차인공대배희석측정。결과설치전측정조평균치위2．49g／L설치후측정조평균치위3．31g／L설치전차인공대배희석측정조평균치위3．33 g／L。결론고제량구상효응사강양성표본오측위약양성，심지가음성결과，고농도표본오측위저치，소이대의기진행설치차대표본배비희석래진행측정，측출래적치경접근진치。
Objective To understand correctly the hook like effect of the immune transmission and transmission turbidity in the automatic biochemical analyzer and the prevention of the hook like effect .Methods First ,it had the function set of measurement linear range of the upper limit of the instrument and the instrument automatic dilution redo ,and then ,had determination of excee‐ding the upper limit of measurement range and artificial dilution before the sample setting .Results The group average value was 2 .49 g/Lbefore the determination setting ,3 .31 g/L after the determination setting ,and 3 .33 g/L artificial dilution determination . Conclusion High dose hook effect makes the strong positive to weak positive samples of measurement by error ,even false negative results ,and high concentration sample makes low value .So the set of samples and dilution to determination of instrument ,measured values are more close to the true value .