河南大学学报（医学版）
河南大學學報（醫學版）
하남대학학보（의학판）
JOURNAL OF HENAN UNIVERSITY (MEDICAL SCIENCE)
2015年
2期
115-118
,共4页
前列腺素E1%缺血再灌注损伤%核因子-B%细胞间黏附分子-1%离体心脏
前列腺素E1%缺血再灌註損傷%覈因子-B%細胞間黏附分子-1%離體心髒
전렬선소E1%결혈재관주손상%핵인자-B%세포간점부분자-1%리체심장
Prostaglandin E1%ischemia reperfusion injury%nuclear transcription factor-B%Intercellular adnesion molecule-1%isolated heart
目的：探讨PGE1预处理对缺血再灌注损伤心肌NF‐κB和ICAM‐1表达的影响，进一步从炎症信号通路揭示PGE1对M IRI保护作用的可能机制。方法40只大鼠随机分为5组：正常对照组、缺血再灌注组（I／R组）、PGE114μg／L预处理组、PGE142μg／L预处理组和PGE1126μg／L预处理组。采用Langendorff大鼠离体心脏灌流装置制备MIRI模型，制备病理切片观察大鼠心肌组织形态学的变化，免疫组化法测定 NF‐κB及ICAM‐1的表达。结果 PGE1各剂量预处理组大鼠缺血再灌注心肌组织形态学变化得到不同程度的改善；NF‐κB及 ICAM‐1的表达在各PGE1预处理组较I／R组明显减少，且各组之间比较有统计学差异（P ＜00．5）。结论 PGE1预处理可以改善大鼠缺血再灌注心肌组织形态学变化，并通过抑制NF‐κB和ICAM‐1的表达减轻心肌中PMN介导的炎症反应，有效减轻离体大鼠M IRI。
目的：探討PGE1預處理對缺血再灌註損傷心肌NF‐κB和ICAM‐1錶達的影響，進一步從炎癥信號通路揭示PGE1對M IRI保護作用的可能機製。方法40隻大鼠隨機分為5組：正常對照組、缺血再灌註組（I／R組）、PGE114μg／L預處理組、PGE142μg／L預處理組和PGE1126μg／L預處理組。採用Langendorff大鼠離體心髒灌流裝置製備MIRI模型，製備病理切片觀察大鼠心肌組織形態學的變化，免疫組化法測定 NF‐κB及ICAM‐1的錶達。結果 PGE1各劑量預處理組大鼠缺血再灌註心肌組織形態學變化得到不同程度的改善；NF‐κB及 ICAM‐1的錶達在各PGE1預處理組較I／R組明顯減少，且各組之間比較有統計學差異（P ＜00．5）。結論 PGE1預處理可以改善大鼠缺血再灌註心肌組織形態學變化，併通過抑製NF‐κB和ICAM‐1的錶達減輕心肌中PMN介導的炎癥反應，有效減輕離體大鼠M IRI。
목적：탐토PGE1예처리대결혈재관주손상심기NF‐κB화ICAM‐1표체적영향，진일보종염증신호통로게시PGE1대M IRI보호작용적가능궤제。방법40지대서수궤분위5조：정상대조조、결혈재관주조（I／R조）、PGE114μg／L예처리조、PGE142μg／L예처리조화PGE1126μg／L예처리조。채용Langendorff대서리체심장관류장치제비MIRI모형，제비병리절편관찰대서심기조직형태학적변화，면역조화법측정 NF‐κB급ICAM‐1적표체。결과 PGE1각제량예처리조대서결혈재관주심기조직형태학변화득도불동정도적개선；NF‐κB급 ICAM‐1적표체재각PGE1예처리조교I／R조명현감소，차각조지간비교유통계학차이（P ＜00．5）。결론 PGE1예처리가이개선대서결혈재관주심기조직형태학변화，병통과억제NF‐κB화ICAM‐1적표체감경심기중PMN개도적염증반응，유효감경리체대서M IRI。
Objective To investigate the effects of NF‐κB and ICAM‐1 expression in preconditioning myocardium with ischemia reperfusion injury and reveal the possible mechanism of PGE 1 protective effect from inflammatory signaling pathways . Methods 40 rats were divided into five groups at random :normal control group , ischemia/reperfusion group and PGE1 14 , 42 , 126 μg/L preconditioning groups . The model of myocardial ischemia reperfusion injury was established in isolated rat heart perfused with Langendorff technique ,the pathological slices were maked to observe the pathomorphological changes of the rat myocardial fibers , the immunohistochemical method was used to measure the changes of NF‐κB and ICAM‐1 expression . Results The morphological changes of ischemia/reperfusion myocardial fiber were improved in PGE1 preconditioning groups . Compared with the I/R group ,the myocardial expression of NF‐κB and ICAM‐1 in PGE1 preconditioning group was significantly reduced (P< 0 .05 ) . Conclusion PGE1 preconditioning can improve the morphological changes of ischemia/reperfusion myocardial fibre , inhibit the expression of NF‐κB and ICAM‐1 in order to effectively reduce the myocardial inflammation mediated PMN and reduce ischemia/reperfusion injury in isolated rat heart .
