湖南师范大学学报（医学版）
湖南師範大學學報（醫學版）
호남사범대학학보（의학판）
JOURNAL OF HUNAN NORMAL UNIVERSITY(MEDICAL SCIENCE)
2015年
2期
4-7
,共4页
周丰%周光华%陈璇
週豐%週光華%陳璇
주봉%주광화%진선
乳腺癌%Fas%基因多态性%基因表达
乳腺癌%Fas%基因多態性%基因錶達
유선암%Fas%기인다태성%기인표체
breast cancer%Fas%SNP%gene expression
目的：探讨 Fas 基因启动子区单核苷酸多态性与乳腺癌遗传易感性的关系。方法：采用病例对照研究，利用 PCR-RFLP 和测序的方法对214例乳腺癌患者与204例健康人对照的 Fas-670A/G 位点进行基因分型。并对检测结果进行χ2检验。通过实时 ELISA 检测 Fas 基因的表达水平。结果：Fas-670A/G 和乳腺癌群体的基因型分布与对照组有显著差异；GG、AA、AG 基因型个体之间 sFas 基因表达水平有显著差异，GG 型个体 Fas 基因的表达水平显著高于 AA、AG 基因型个体。结论：乳腺癌组中 Fas 基因的表达显著增加，Fas-670A/G 多态性位点通过调节 Fas 基因的表达与女性患乳腺癌发病风险显著相关。且 GG 基因型增强了 Fas 基因的表达，增加了发病风险。
目的：探討 Fas 基因啟動子區單覈苷痠多態性與乳腺癌遺傳易感性的關繫。方法：採用病例對照研究，利用 PCR-RFLP 和測序的方法對214例乳腺癌患者與204例健康人對照的 Fas-670A/G 位點進行基因分型。併對檢測結果進行χ2檢驗。通過實時 ELISA 檢測 Fas 基因的錶達水平。結果：Fas-670A/G 和乳腺癌群體的基因型分佈與對照組有顯著差異；GG、AA、AG 基因型箇體之間 sFas 基因錶達水平有顯著差異，GG 型箇體 Fas 基因的錶達水平顯著高于 AA、AG 基因型箇體。結論：乳腺癌組中 Fas 基因的錶達顯著增加，Fas-670A/G 多態性位點通過調節 Fas 基因的錶達與女性患乳腺癌髮病風險顯著相關。且 GG 基因型增彊瞭 Fas 基因的錶達，增加瞭髮病風險。
목적：탐토 Fas 기인계동자구단핵감산다태성여유선암유전역감성적관계。방법：채용병례대조연구，이용 PCR-RFLP 화측서적방법대214례유선암환자여204례건강인대조적 Fas-670A/G 위점진행기인분형。병대검측결과진행χ2검험。통과실시 ELISA 검측 Fas 기인적표체수평。결과：Fas-670A/G 화유선암군체적기인형분포여대조조유현저차이；GG、AA、AG 기인형개체지간 sFas 기인표체수평유현저차이，GG 형개체 Fas 기인적표체수평현저고우 AA、AG 기인형개체。결론：유선암조중 Fas 기인적표체현저증가，Fas-670A/G 다태성위점통과조절 Fas 기인적표체여녀성환유선암발병풍험현저상관。차 GG 기인형증강료 Fas 기인적표체，증가료발병풍험。
Objective To evaluate the association between polymorphisms in Fas promoter region and susceptibility to breast cancer. Methods In the case-control study, we genotyped Fas-670A/G and Fas-1377A/G by PCR-RFLP in 214 pa-tients with breast cancer and 204 healthy controls. Results were analyzed using Chi-square test. Fas gene expression wwas detected by q-RT-PCR in breast cancer. Results The distribution of Fas-670A/G genotype and allele type was significant difference between the patients and healthy controls. Subjects with GG genotype significantly increased the risk of breast can-cer compared with the individuals carrying AA and AG genotype. Fas expression level in subjects with breast cancer was sig-nificantly increased compared with the healthy control. And the Fas expression in subjects with GG genotype was significantly higher than that in subjects with AA, GG genotypes. Conclusion Fas expression significantly increased in breast cancer. Fas was regulated by Fas-670A/G and significantly associated with susceptibility to breast cancer. GG genotype enhanced Fas ex-pression and increased the risk to breast cancer.
