CAJ | 학술논문

目的：探究柴胡皂苷B2对人神经母细胞瘤细胞（SH －SY5Y）过氧化氢损伤后的作用。方法采用体外细胞培养的方法，建立 SH －SY5Y神经细胞 H2 O2损伤模型。分别将含体积分数0.05、0．10、0．20柴胡皂苷B2（10－4 mol／L）的小牛血清按10％的比例加入SH －SY5Y细胞中，然后采用140μmol／L的H2 O2进行处理，平行设立10％小牛血清组和空白血清未经H2 O2处理组作为模型组和空白组。观察各组细胞形态，同时测定细胞存活率，检测柴胡皂苷B2作用下SH －SY5Y神经细胞的生存状态。结果同模型组比较，1×10－6 mol／L和2×10－6 mol／L的柴胡皂苷B2均能显著减轻 H2 O2造成的S H －SY5Y神经细胞的过氧损伤，明显提高细胞的存活率。结论柴胡皂苷B2对过氧化氢诱导损伤的人神经母细胞瘤细胞具有明显的保护作用。
목적：탐구시호조감B2대인신경모세포류세포（SH －SY5Y）과양화경손상후적작용。방법채용체외세포배양적방법，건립 SH －SY5Y신경세포 H2 O2손상모형。분별장함체적분수0.05、0．10、0．20시호조감B2（10－4 mol／L）적소우혈청안10％적비례가입SH －SY5Y세포중，연후채용140μmol／L적H2 O2진행처리，평행설립10％소우혈청조화공백혈청미경H2 O2처리조작위모형조화공백조。관찰각조세포형태，동시측정세포존활솔，검측시호조감B2작용하SH －SY5Y신경세포적생존상태。결과동모형조비교，1×10－6 mol／L화2×10－6 mol／L적시호조감B2균능현저감경 H2 O2조성적S H －SY5Y신경세포적과양손상，명현제고세포적존활솔。결론시호조감B2대과양화경유도손상적인신경모세포류세포구유명현적보호작용。
Objective To investigate the effect of Saikosaponin B2 on the damage of cultured SH -SY5Y cells. Methods Damaged SH -SY5Y cells model was established with the method of cell culture in vitro. 10% calf serum including volume fraction 0. 05 ,0. 10 ,0. 20 Saikosaponin B2 (10-4 mol/L) were added respectively into the SH -SY5Y cells ,which were then treated with 140μmol/L hydrogen perox‐ide (H2 O2 ). 10% calf serum group and blank serum without H2 O2 -treated group were as the model group and the control group. The effect of Saikosaponin B2 was observed by morphological identification , colorimetric MTT assay. Results Both Saikosaponin B2 of 1 × 10-6 mol/L and 2 × 10-6 mol/L can relieve the damage of SH -SY5Y cells and increase the survival of the cells. Conclusions Saikosaponin B2 can protect the cultured SH -SY5Y cells from damage induced by H2 O2.