山东体育学院学报
山東體育學院學報
산동체육학원학보
JOURNAL OF SHANDONG PHYSICAL EDUCATION INSTITUTE
2015年
3期
73-77
,共5页
有氧运动%心肌梗死%神经重构%炎症
有氧運動%心肌梗死%神經重構%炎癥
유양운동%심기경사%신경중구%염증
aerobic exercise%myocardial infarction%neural remodeling%inflammation
目的：探讨8周有氧运动对心肌梗死大鼠心脏交感重构的影响及其机制。方法：42只健康雄性SD大鼠随机分为假手术组、心肌梗死组、心肌梗死+有氧运动组。结扎冠状动脉左前降支制备大鼠心肌梗死的模型，术后1周心肌梗死+有氧运动组成活大鼠进行8周跑台运动。采用免疫组织化学方法观察心肌中GAP43、TH阳性神经纤维分布及表达；采用western blot检测心肌中NGF蛋白表达；采用实时定量RT-PCR检测IL-1β及TNF-α mRNA表达。结果：与假手术组大鼠相比，心肌梗死组心肌中GAP43、TH阳性神经纤维密度明显增加（ P＜0.01），形态粗大且空间分布紊乱；NGF蛋白，IL-1β及TNF-α mRNA表达显著上调。有氧运动干预后心肌组织神经纤维密度明显降低（ P＜0.01），形态更趋于正常；心肌中NGF、IL-1β及TNF-α表达显著下降。结论：有氧运动减少心肌梗死后心肌局部炎症反应，下调梗死灶周组织NGF表达，抑制交感神经过度再生，从而改善心肌梗死后交感神经重构。
目的：探討8週有氧運動對心肌梗死大鼠心髒交感重構的影響及其機製。方法：42隻健康雄性SD大鼠隨機分為假手術組、心肌梗死組、心肌梗死+有氧運動組。結扎冠狀動脈左前降支製備大鼠心肌梗死的模型，術後1週心肌梗死+有氧運動組成活大鼠進行8週跑檯運動。採用免疫組織化學方法觀察心肌中GAP43、TH暘性神經纖維分佈及錶達；採用western blot檢測心肌中NGF蛋白錶達；採用實時定量RT-PCR檢測IL-1β及TNF-α mRNA錶達。結果：與假手術組大鼠相比，心肌梗死組心肌中GAP43、TH暘性神經纖維密度明顯增加（ P＜0.01），形態粗大且空間分佈紊亂；NGF蛋白，IL-1β及TNF-α mRNA錶達顯著上調。有氧運動榦預後心肌組織神經纖維密度明顯降低（ P＜0.01），形態更趨于正常；心肌中NGF、IL-1β及TNF-α錶達顯著下降。結論：有氧運動減少心肌梗死後心肌跼部炎癥反應，下調梗死竈週組織NGF錶達，抑製交感神經過度再生，從而改善心肌梗死後交感神經重構。
목적：탐토8주유양운동대심기경사대서심장교감중구적영향급기궤제。방법：42지건강웅성SD대서수궤분위가수술조、심기경사조、심기경사+유양운동조。결찰관상동맥좌전강지제비대서심기경사적모형，술후1주심기경사+유양운동조성활대서진행8주포태운동。채용면역조직화학방법관찰심기중GAP43、TH양성신경섬유분포급표체；채용western blot검측심기중NGF단백표체；채용실시정량RT-PCR검측IL-1β급TNF-α mRNA표체。결과：여가수술조대서상비，심기경사조심기중GAP43、TH양성신경섬유밀도명현증가（ P＜0.01），형태조대차공간분포문란；NGF단백，IL-1β급TNF-α mRNA표체현저상조。유양운동간예후심기조직신경섬유밀도명현강저（ P＜0.01），형태경추우정상；심기중NGF、IL-1β급TNF-α표체현저하강。결론：유양운동감소심기경사후심기국부염증반응，하조경사조주조직NGF표체，억제교감신경과도재생，종이개선심기경사후교감신경중구。
Objective:To investigate the role of aerobic exercise in ameliorating sympathetic sprouting after myocardial infarction( MI)in rat hearts. Methods:42 male Sprague -Dawley rats were ran-domly divided into three groups:sham group,MI and MI with aerobic exercise( MI+ME)group. An MI model was induced by ligation of the coronary artery;the rats in MI+ME group were as-signed to 8 weeks of exercise. Immunohistochemistry was used to measure the density of nerve fibers positive for growth-associated protein 43( GAP43 )and tyrosine hydroxylase( TH ). The protein levels of nerve growth factor( NGF)were detected by western blot analysis. The mRNA levels of in-terleukin-1β( IL-1β)and tumor necrosis factor-α( TNF-α)were examined by quantitative real-time PCR. Results:Compared with the sham-operated hearts,MI increased the density of GAP43- and TH -positive fibers,moreover,the distribution and structure of regenerated nerve was heterogeneous in MI hearts. Moreover,the levels of NGF,IL-1βand TNF-αwere upregulat-ed in MI as compared with sham-operated hearts. Aerobic exercise treatment ameliorated sympa-thetic hyperinnervation after MI;the levels of NGF,IL-1β and TNF-α were significantly down-regulated with MI+ME than MI alone. Conclusions:Aerobic exercise reduced inflammatory reaction caused by MI,downregulated NGF expression and inhibited sympathetic hyperinnervation,which may be a potential mechanism for ameliorating sympathetic neural remodeling in rat.
