福建农业学报
福建農業學報
복건농업학보
FUJIAN JOURNAL OF AGRICULTURAL SCIENCES
2015年
4期
351-356
,共6页
杨德卫%叶宁%叶新福%郑向华%程朝平%卢礼斌%黄凤凰
楊德衛%葉寧%葉新福%鄭嚮華%程朝平%盧禮斌%黃鳳凰
양덕위%협저%협신복%정향화%정조평%로례빈%황봉황
水稻%恢复系%白叶枯病%Xa23%分子标记辅助选择
水稻%恢複繫%白葉枯病%Xa23%分子標記輔助選擇
수도%회복계%백협고병%Xa23%분자표기보조선택
rice%restore line%bacterial blight%X a23 gene%marker assisted selection
利用常规杂交和回交的方法,并通过分子标记辅助选择技术,以携带抗白叶枯病基因 X a23水稻材料IRBBL23为供体材料,以优良早稻恢复系东南恢012的为受体材料,在低世代利用与 X a23基因紧密连锁的分子标记C189在分离群体中进行分子标记辅助选择,在高代稳定株系中采用苗期群体鉴定技术,最终获得一系列目标基因纯合且农艺性状优良的早熟恢复系材料。通过白叶枯病菌株的专化强毒菌系P6对8份材料进行田间接种鉴定,筛选出7份抗白叶枯病的恢复系材料,并对这些株系的主要农艺性状进行了调查和分析。结果表明利用与 X a23基因紧密连锁的分子标记C189开展抗白叶枯病分子标记辅助育种是一种简便、有效的途径。
利用常規雜交和迴交的方法,併通過分子標記輔助選擇技術,以攜帶抗白葉枯病基因 X a23水稻材料IRBBL23為供體材料,以優良早稻恢複繫東南恢012的為受體材料,在低世代利用與 X a23基因緊密連鎖的分子標記C189在分離群體中進行分子標記輔助選擇,在高代穩定株繫中採用苗期群體鑒定技術,最終穫得一繫列目標基因純閤且農藝性狀優良的早熟恢複繫材料。通過白葉枯病菌株的專化彊毒菌繫P6對8份材料進行田間接種鑒定,篩選齣7份抗白葉枯病的恢複繫材料,併對這些株繫的主要農藝性狀進行瞭調查和分析。結果錶明利用與 X a23基因緊密連鎖的分子標記C189開展抗白葉枯病分子標記輔助育種是一種簡便、有效的途徑。
이용상규잡교화회교적방법,병통과분자표기보조선택기술,이휴대항백협고병기인 X a23수도재료IRBBL23위공체재료,이우량조도회복계동남회012적위수체재료,재저세대이용여 X a23기인긴밀련쇄적분자표기C189재분리군체중진행분자표기보조선택,재고대은정주계중채용묘기군체감정기술,최종획득일계렬목표기인순합차농예성상우량적조숙회복계재료。통과백협고병균주적전화강독균계P6대8빈재료진행전간접충감정,사선출7빈항백협고병적회복계재료,병대저사주계적주요농예성상진행료조사화분석。결과표명이용여 X a23기인긴밀련쇄적분자표기C189개전항백협고병분자표기보조육충시일충간편、유효적도경。
The bacterial blight is one of the major diseases in rice .One effective control measure for bacterial blight is to breed resistance cultivars through molecular marker closely linked to the resistance genes .The rice bacterial blight resistance gene Xa23 ,carried by the donor parent IRBB23 ,was introduced into the restorer line Dongnanhui 012'by regular and back crossing and molecular( marker assisted selection .This research was carried out in low generation without resistance using molecular marker assisted selection with marker C189 closely linked to Xa23 gene ,and in high and stable strains using seedling population identification technology .A series of precocious restore lines with homozygous target genes X a23 and stable good agronomic characters were obtained through testing the flanking marker C189. The eight lines were subject to bacterial blight disease inoculation at the booting stages ,and results showed that seven lines were resistant to the most destructive Xoo strain PXO 99(P6) .Meanwhile ,we also analyzed the main agronomic traits of the seven homozygous lines . These results clearly suggested that the marker C189 linked to Xa23 could be conveniently and effectively used in the bacterial blight resistance breeding programs of rice .