CAJ | 학술논문

目的：评估外用抗真菌药膏对常见皮肤癣菌及球形孢子丝菌、裴氏着色真菌的体外抑菌活性。方法纳入皮肤常见致病真菌临床分离株7种19株，制作含菌琼脂平板，采用改良琼脂扩散法加入实验药膏，适当培养，观察菌株生长情况并测定抑菌圈直径。结果1％特比萘芬乳膏对所有实验菌株均形成抑菌圈，对常见皮肤癣菌、球形孢子丝菌(酵母相与菌丝相)和裴氏着色真菌的抑菌圈直径大于2％酮康唑、1％联苯苄唑乳膏( P<0?05)；1％联苯苄唑乳膏对于球形孢子丝菌、断发毛癣菌无抑菌圈。结论对于常见皮肤癣菌、球形孢子丝菌和裴氏着色真菌，1％特比萘芬乳膏较2％酮康唑乳膏、1％联苯苄唑乳膏的抗真菌谱更广，抗真菌活性更强。本研究所采用的改良琼脂扩散法成本低，重复性好，简单易操作、贴近临床实际，可为外用抗真菌药物的临床应用提供客观科学的实验依据。
목적：평고외용항진균약고대상견피부선균급구형포자사균、배씨착색진균적체외억균활성。방법납입피부상견치병진균림상분리주7충19주，제작함균경지평판，채용개량경지확산법가입실험약고，괄당배양，관찰균주생장정황병측정억균권직경。결과1％특비내분유고대소유실험균주균형성억균권，대상견피부선균、구형포자사균(효모상여균사상)화배씨착색진균적억균권직경대우2％동강서、1％련분변서유고( P<0?05)；1％련분변서유고대우구형포자사균、단발모선균무억균권。결론대우상견피부선균、구형포자사균화배씨착색진균，1％특비내분유고교2％동강서유고、1％련분변서유고적항진균보경엄，항진균활성경강。본연구소채용적개량경지확산법성본저，중복성호，간단역조작、첩근림상실제，가위외용항진균약물적림상응용제공객관과학적실험의거。
Objective To compare in vitro antifungal activities of three topical antifungal creams on common der￣matophytes,Sporothrix globosa and Fonsecaea pedrosoi. Methods Nineteen pathogenic fungi from seven clinically i￣solated species were used in the study. A modified agar diffusion assay was performed by the modified disk diffusion susceptibility method. Growth status of the isolates and the diameters of the inhibition zones around drug-containing wells were recorded by using photography following an incubation period. Results The inhibition zones of 1% terbin￣afine cream on common dermatophytes,Sporothrix globosa and Fonsecaea pedrosoi were significantly larger than those of the other two creams. No inhibition zones were observed around the 1% bifonazole cream in S globosa and Tricho￣phyton tonsurans. Conclusion The 1% terbinafine cream was more effective in treating common dermatophytes than 1% bifonazole or 2% ketoconazole creams. The modified agar diffusion method is a simple,reproducible,cheap and easily adaptable assay for clinical use.