CAJ | 학술논문

目的：研究重楼提取物单体重楼皂苷Ⅱ在体外对A375人黑素瘤细胞增殖和凋亡的影响。方法体外培养A375人黑素瘤细胞株，采用MTT法检测重楼皂苷Ⅱ对细胞增殖的影响，计算IC50值；透射电镜观察细胞超微结构的变化；采用流式细胞术检测重楼皂苷Ⅱ干预后A375人黑素瘤细胞凋亡的情况。结果重楼皂苷Ⅱ对A375人黑素瘤细胞的增殖有明显的抑制作用，24h的IC50值为12?04μmol/L。重楼皂苷Ⅱ对人A375细胞生长的抑制作用表现出时间和浓度依赖性。另外重楼皂苷Ⅱ引起A375黑素瘤细胞的凋亡率显著增加。结论重楼皂苷Ⅱ对A375人黑素瘤有潜在的抗癌作用，可能是通过诱导黑素瘤细胞凋亡而发挥作用。
목적：연구중루제취물단체중루조감Ⅱ재체외대A375인흑소류세포증식화조망적영향。방법체외배양A375인흑소류세포주，채용MTT법검측중루조감Ⅱ대세포증식적영향，계산IC50치；투사전경관찰세포초미결구적변화；채용류식세포술검측중루조감Ⅱ간예후A375인흑소류세포조망적정황。결과중루조감Ⅱ대A375인흑소류세포적증식유명현적억제작용，24h적IC50치위12?04μmol/L。중루조감Ⅱ대인A375세포생장적억제작용표현출시간화농도의뢰성。령외중루조감Ⅱ인기A375흑소류세포적조망솔현저증가。결론중루조감Ⅱ대A375인흑소류유잠재적항암작용，가능시통과유도흑소류세포조망이발휘작용。
Objective To investigate the potential effect of polyphyllin Ⅱ( PPⅡ) on the proliferation and apop￣tosis of A375 human melanoma cell. Methods A375 Human melanoma cells were cultured with or without PP Ⅱ. The proliferation of cells was measured by MTT assay. The changes of cell morphology were observed by transmission electron microscopy. The apoptosis and cell cycle were measured by flow cytometry. Results The results have shown that the proliferation of A375 human melanoma cells were significantly inhibited by PP Ⅱ. The 50% inhibitory con￣centration ( IC50) of PP Ⅱ were 12.04μmol/L at 24 h. Conclusion The inhibition of PP Ⅱ to melanoma cell was in a dose and time-dependent manner. After being treated with PP Ⅱ, the apoptotic rate of cells was remarkably higher than that of the control. Polyphyllin Ⅱ potentially has anticancer effects on A375 human melanoma cells in vitro,which result from increased cell apoptosis.