CAJ | 학술논문

以细菌菌落计数方法，研究大肠埃希菌、金黄色葡萄球菌、鼠伤寒沙门氏菌在不同2，3，5－三苯基氯化四氮唑（TTC）浓度的平板计数琼脂上菌落形态和数量的变化情况。结果表明，3种菌在TTC终浓度≥0.0008％时，菌落颜色比较明显，逐渐加深；大肠埃希菌在TTC终浓度≤0.01％的平板计数琼脂上的菌落数量和对照组无显著差异，TTC终浓度≥0.02％菌落数量明显减少；金黄色葡萄球菌在TTC终浓度≤0.004％的平板计数琼脂上的菌落数量和对照组无显著差异，在0.04％ TTC平板计数琼脂上不能生长；鼠伤寒沙门氏菌TTC终浓度≤0.01％的平板计数琼脂上的菌落数量和对照组无显著差异，在TTC终浓度≥0.02％的平板计数琼脂上菌落数量减少。不同浓度的TTC对鼠伤寒沙门氏菌、大肠埃希菌的生长影响较小，对金黄色葡萄球菌影响较大，故在食品（或饲料）细菌总数检测中，TTC的终浓度应选在0.0008％～0.004％之间。
이세균균락계수방법，연구대장애희균、금황색포도구균、서상한사문씨균재불동2，3，5－삼분기록화사담서（TTC）농도적평판계수경지상균락형태화수량적변화정황。결과표명，3충균재TTC종농도≥0.0008％시，균락안색비교명현，축점가심；대장애희균재TTC종농도≤0.01％적평판계수경지상적균락수량화대조조무현저차이，TTC종농도≥0.02％균락수량명현감소；금황색포도구균재TTC종농도≤0.004％적평판계수경지상적균락수량화대조조무현저차이，재0.04％ TTC평판계수경지상불능생장；서상한사문씨균TTC종농도≤0.01％적평판계수경지상적균락수량화대조조무현저차이，재TTC종농도≥0.02％적평판계수경지상균락수량감소。불동농도적TTC대서상한사문씨균、대장애희균적생장영향교소，대금황색포도구균영향교대，고재식품（혹사료）세균총수검측중，TTC적종농도응선재0.0008％～0.004％지간。
According to bacterial colony counting method ,we researched the changes of bacterial colony morphology and quantity of Escherichia coli ,Staphylococcus aureus and Salmonella typhimurium in different concentrations of TTC agarplate .The results showed that the color of bacte-rial colony of these three strains was obvious and became darkening when TTC final concentration ≥0.0008% ;In TTC final concentration ≤0.01% ,the bacterial colony number of E .coli was no significant difference from that of the control group .However ,the number of colonies were significantly reduced in TTC final concentration ≥0.02% ;In TTC final concentration ≤0.004% ,the bacterial colony number of Staphylococ-cus aureus was no significant difference from that of the control group;In addition ,the bacterial colony could not grow in the medium of TTC's concentration reaching to 0.04% ;When TTC final concentration ≤0.01% ,the bacterial colony number of Salmonella typhi was no significant difference from that of the control group .But when TTC final concentration ≥0.02% ,the number of colonies was reduced .Conclusions:Differ-ent TTC concentrations have less effect on the growth of Salmonella typhi and E .coli ,while they have greater impact on the growth of Staphylo-coccus aureus .So in the detection of total bacteria number in food(or fodder) ,TTC final concentration should be chosen between 0.0008% and 0.004% .