河南农业科学
河南農業科學
하남농업과학
JOURNAL OF HENAN AGRICULTURAL SCIENCES
2015年
6期
105-108
,共4页
大葱根系浸提液%化感效应%樱桃萝卜%化感作用抑制率%抗氧化酶%丙二醛
大蔥根繫浸提液%化感效應%櫻桃蘿蔔%化感作用抑製率%抗氧化酶%丙二醛
대총근계침제액%화감효응%앵도라복%화감작용억제솔%항양화매%병이철
root extract of Allium fistulosum%allelopathic effect%Raphanus sativus%inhibitory rates%an-tioxidant enzymes%malonaldehyde
为了探明大葱茬地块对樱桃萝卜生长及生理机制的影响,采用不同质量浓度大葱根系水浸提液处理樱桃萝卜种子,进行发芽试验后测定樱桃萝卜幼苗的生长、抗氧化酶活性及丙二醛( MDA)含量。结果表明:不同质量浓度大葱根系浸提液对樱桃萝卜幼苗化感效应有不同影响。大葱根系浸提液20~30 g/L对樱桃萝卜种子发芽、10~30 g/L对樱桃萝卜幼苗胚根长均表现为极显著的化感抑制作用,而<10 g/L影响不明显,但对苗高始终表现为化感促进作用。与蒸馏水处理(对照)相比,随着大葱根系浸提液质量浓度的升高,过氧化物酶( POD)活性先升高后下降,过氧化氢酶( CAT)活性在浸提液10~30 g/L时极显著降低,而超氧化物歧化酶( SOD)活性无显著变化;对POD、SOD、CAT活性的总化感作用抑制率统计表明,浸提液质量浓度为5~20 g/L时,酶总活性受化感作用促进,而30 g/L时则被抑制;浸提液5~30 g/L处理的MDA含量高于对照,其中20~30 g/L处理与对照差异显著。表明大葱根系浸提液对樱桃萝卜种子发芽及幼苗生长具有化感抑制作用。樱桃萝卜幼苗通过增强POD活性清除体内氧自由基,但是随处理浓度升高,抗氧化酶活性均受抑制,膜质过氧化程度加剧。
為瞭探明大蔥茬地塊對櫻桃蘿蔔生長及生理機製的影響,採用不同質量濃度大蔥根繫水浸提液處理櫻桃蘿蔔種子,進行髮芽試驗後測定櫻桃蘿蔔幼苗的生長、抗氧化酶活性及丙二醛( MDA)含量。結果錶明:不同質量濃度大蔥根繫浸提液對櫻桃蘿蔔幼苗化感效應有不同影響。大蔥根繫浸提液20~30 g/L對櫻桃蘿蔔種子髮芽、10~30 g/L對櫻桃蘿蔔幼苗胚根長均錶現為極顯著的化感抑製作用,而<10 g/L影響不明顯,但對苗高始終錶現為化感促進作用。與蒸餾水處理(對照)相比,隨著大蔥根繫浸提液質量濃度的升高,過氧化物酶( POD)活性先升高後下降,過氧化氫酶( CAT)活性在浸提液10~30 g/L時極顯著降低,而超氧化物歧化酶( SOD)活性無顯著變化;對POD、SOD、CAT活性的總化感作用抑製率統計錶明,浸提液質量濃度為5~20 g/L時,酶總活性受化感作用促進,而30 g/L時則被抑製;浸提液5~30 g/L處理的MDA含量高于對照,其中20~30 g/L處理與對照差異顯著。錶明大蔥根繫浸提液對櫻桃蘿蔔種子髮芽及幼苗生長具有化感抑製作用。櫻桃蘿蔔幼苗通過增彊POD活性清除體內氧自由基,但是隨處理濃度升高,抗氧化酶活性均受抑製,膜質過氧化程度加劇。
위료탐명대총치지괴대앵도라복생장급생리궤제적영향,채용불동질량농도대총근계수침제액처리앵도라복충자,진행발아시험후측정앵도라복유묘적생장、항양화매활성급병이철( MDA)함량。결과표명:불동질량농도대총근계침제액대앵도라복유묘화감효응유불동영향。대총근계침제액20~30 g/L대앵도라복충자발아、10~30 g/L대앵도라복유묘배근장균표현위겁현저적화감억제작용,이<10 g/L영향불명현,단대묘고시종표현위화감촉진작용。여증류수처리(대조)상비,수착대총근계침제액질량농도적승고,과양화물매( POD)활성선승고후하강,과양화경매( CAT)활성재침제액10~30 g/L시겁현저강저,이초양화물기화매( SOD)활성무현저변화;대POD、SOD、CAT활성적총화감작용억제솔통계표명,침제액질량농도위5~20 g/L시,매총활성수화감작용촉진,이30 g/L시칙피억제;침제액5~30 g/L처리적MDA함량고우대조,기중20~30 g/L처리여대조차이현저。표명대총근계침제액대앵도라복충자발아급유묘생장구유화감억제작용。앵도라복유묘통과증강POD활성청제체내양자유기,단시수처리농도승고,항양화매활성균수억제,막질과양화정도가극。
In order to understand the effects of stubble of Allium fistulosum on growth and physiological mechanisms of Raphanus sativus,the growth,antioxidant enzyme activity and malonaldehyde( MDA) con-tent of R. sativus seedling were measured by germination test in different concentrations of root extract of A. fistulosum. The results showed that there were different effects on the allelopathic effects of different concentrations root extract of A. fistulosum on R. sativus seedlings. The germination of R. sativus were in-hibited(P<0. 01) by root extract of A. fistulosum when the concentration of extract were between 20 g/L and 30 g/L. The root length of R. sativus were inhibited(P<0. 01) when the concentration of root extract were between 10 g/L and 30 g/L. And there were no significant difference when the concentration of root extract was less than 10 g/L compare with control. However,the seedling height was promoted by the root extract of A. fistulosum. With the increase of root extract concentration,the peroxide enzyme( POD) activi-ty increased first and then decreased;and catalase( CAT) activity decreased significantly when the con-centration of root extract were between 10 g/L and 30 g/L;there were no significant difference for the ac-tivities of superoxide dismutase( SOD) between the root extract treatments and the control. And total ac-tivities of the three antioxidant enzymes were increased by allelopathy when the concentrations of root ex-tract were between 5 g/L and 20 g/L,and inhibited when the concentrations of root extract was 30 g/L. The contents of MDA were higher than the control when the the concentrations of root extract were be-tween 5 g/L and 30 g/L,and reaching significant levels from 20 g/L to 30 g/L. The above results showed that there was an allelopathic inhibition of A. fistulosum root extract on seed germination and seedling growth of R. sativus. Furthermore,oxygen radical in R. sativus were eliminated by increasing POD activi-ties. However,with the increase of root extract concentration,antioxidant enzymes activities were inhibi-ted,so the peroxidization of cytomembrane would aggravate.