中国现代医生
中國現代醫生
중국현대의생
CHINA MODERN DOCTOR
2015年
15期
4-7
,共4页
蓝德云%焦军强%王永波%陶学育
藍德雲%焦軍彊%王永波%陶學育
람덕운%초군강%왕영파%도학육
死亡受体%基因多态性%溃疡性结肠炎
死亡受體%基因多態性%潰瘍性結腸炎
사망수체%기인다태성%궤양성결장염
Death receptor%Gene polymorphism%Ulcerative colitis
目的:探讨死亡受体(阅砸)4基因多态性与溃疡性结肠炎(UC)易感性及其临床病理特征的关系。方法在147例UC患者和244名健康对照者中,采用直接测序法检测DR4(rs20575)基因单核苷酸多态性。采用非条件Logistic回归分析DR4(rs20575)基因多态性与UC的关系。结果与对照组比较,UC组DR4(rs20575)突变等位基因(G)和基因型(CG+GG)的频率明显升高(5.78% vs 1.23%,P=0.001,OR=4.930,95%CI:1.192~12.651;7.48% vs 2.46%,P=0.025,OR=3.208,95%CI:1.161~8.869)。根据改良的Truelove&Witts严重程度分型标准将UC患者分为轻度、中度和重度。结果显示中重度UC患者中DR4(rs20575)突变等位基因(G)和基因型(CG+GG)的频率显著高于轻度患者(10.64%vs 3.50%,P=0.020,OR=3.282,95%CI:1.208~8.916;14.89% vs 4.00%,P=0.028,OR=4.200,95%CI:1.165~15.146)。 DR4(rs20575)的突变等位基因和基因型频率在广泛性结肠炎和远端结肠炎之间均无统计学差异(P>0.05)。结论DR4(rs20575)基因多态性与UC易感性密切相关,且可能影响UC的病情严重程度。
目的:探討死亡受體(閱砸)4基因多態性與潰瘍性結腸炎(UC)易感性及其臨床病理特徵的關繫。方法在147例UC患者和244名健康對照者中,採用直接測序法檢測DR4(rs20575)基因單覈苷痠多態性。採用非條件Logistic迴歸分析DR4(rs20575)基因多態性與UC的關繫。結果與對照組比較,UC組DR4(rs20575)突變等位基因(G)和基因型(CG+GG)的頻率明顯升高(5.78% vs 1.23%,P=0.001,OR=4.930,95%CI:1.192~12.651;7.48% vs 2.46%,P=0.025,OR=3.208,95%CI:1.161~8.869)。根據改良的Truelove&Witts嚴重程度分型標準將UC患者分為輕度、中度和重度。結果顯示中重度UC患者中DR4(rs20575)突變等位基因(G)和基因型(CG+GG)的頻率顯著高于輕度患者(10.64%vs 3.50%,P=0.020,OR=3.282,95%CI:1.208~8.916;14.89% vs 4.00%,P=0.028,OR=4.200,95%CI:1.165~15.146)。 DR4(rs20575)的突變等位基因和基因型頻率在廣汎性結腸炎和遠耑結腸炎之間均無統計學差異(P>0.05)。結論DR4(rs20575)基因多態性與UC易感性密切相關,且可能影響UC的病情嚴重程度。
목적:탐토사망수체(열잡)4기인다태성여궤양성결장염(UC)역감성급기림상병리특정적관계。방법재147례UC환자화244명건강대조자중,채용직접측서법검측DR4(rs20575)기인단핵감산다태성。채용비조건Logistic회귀분석DR4(rs20575)기인다태성여UC적관계。결과여대조조비교,UC조DR4(rs20575)돌변등위기인(G)화기인형(CG+GG)적빈솔명현승고(5.78% vs 1.23%,P=0.001,OR=4.930,95%CI:1.192~12.651;7.48% vs 2.46%,P=0.025,OR=3.208,95%CI:1.161~8.869)。근거개량적Truelove&Witts엄중정도분형표준장UC환자분위경도、중도화중도。결과현시중중도UC환자중DR4(rs20575)돌변등위기인(G)화기인형(CG+GG)적빈솔현저고우경도환자(10.64%vs 3.50%,P=0.020,OR=3.282,95%CI:1.208~8.916;14.89% vs 4.00%,P=0.028,OR=4.200,95%CI:1.165~15.146)。 DR4(rs20575)적돌변등위기인화기인형빈솔재엄범성결장염화원단결장염지간균무통계학차이(P>0.05)。결론DR4(rs20575)기인다태성여UC역감성밀절상관,차가능영향UC적병정엄중정도。
Objective To explore the association between death receptor (DR) 4 gene polymorphism and the predis-position of ulcerative colitis(UC). Methods DR4(rs20575)genotypes were determined by direct sequencing in a total of 147 UC patients and 244 healthy controls. Logistic regression analysis was employed to assess the relationship be-tween DR4 (rs20575)gene polymorphism and UC. Results Compared to the controls, the frequencies of variant allele(G)and genotype(CG+GG) of DR4(rs20575)were significantly increased in UC patients(5.78% vs 1.23%,P=0.001,OR=4.930,95%CI1.192-12.651;7.48% vs 2.46%,P=0.025,OR=3.208,95%CI1.161-8.869). According to the Tru-elove &Witt Activity Index, the severity of UC was further stratified into the mild, moderate and severe disease. In re-sult, variant allele (G)and genotype(CG+GG)in DR4(rs20575)were significantly higher in patients with moderate and severe UC than those with mild UC (10.64% vs 3.50%,P=0.020,OR=3.282,95%CI1.208-8.916; 14.89% vs 4.00%,P=0.028,OR=4.200,95%CI1.165-15.146). DR4(rs20575) mutant allele and genotype frequencies between extensive colitis and distal colitis were no statistical difference(P>0.05). Conclusion The genetic polymorphism of DR4(rs20575)is significantly related with the susceptibility to UC, as well as severity of the disease in this cohort of Chinese pa-tients.