CAJ | 학술논문

目的：检测妊娠期肝内胆汁淤积症（ ICP）患者胎盘组织缺氧诱导因子HIF-2α蛋白的含量，探讨其在ICP胎儿宫内缺氧中的作用。方法：选取2010年2～8月在弋矶山医院剖宫产分娩的40例孕妇为研究对象，其中正常足月孕妇10例，ICP轻度组15例，重度组15例。采用蛋白免疫印迹法检测各组孕妇胎盘组织中HIF-2α蛋白的表达水平。结果：3组孕妇HIF-2α蛋白表达差异有统计学意义（P＜0．05），轻、重度ICP组HIF-2α蛋白表达较正常对照组有统计学意义（P＜0．05）；轻、重度ICP组间HIF-2α蛋白表达差异无统计学意义（P＞0．05）。结论：ICP患者HIF-2α蛋白在胎盘组织中的高表达，可能在胎盘-胎儿系统对缺氧的自稳和代偿反应中起重要的调节作用。
목적：검측임신기간내담즙어적증（ ICP）환자태반조직결양유도인자HIF-2α단백적함량，탐토기재ICP태인궁내결양중적작용。방법：선취2010년2～8월재익기산의원부궁산분면적40례잉부위연구대상，기중정상족월잉부10례，ICP경도조15례，중도조15례。채용단백면역인적법검측각조잉부태반조직중HIF-2α단백적표체수평。결과：3조잉부HIF-2α단백표체차이유통계학의의（P＜0．05），경、중도ICP조HIF-2α단백표체교정상대조조유통계학의의（P＜0．05）；경、중도ICP조간HIF-2α단백표체차이무통계학의의（P＞0．05）。결론：ICP환자HIF-2α단백재태반조직중적고표체，가능재태반-태인계통대결양적자은화대상반응중기중요적조절작용。
Objective:To investigate the role of hypoxia-inducible factor-2α(HIF-2α) protein in placenta in intrahepatic cholestasis of pregnancy(ICP). Methods:Forty women undergone cesarean section delivery were included between February and August of 2010 in our hospital.Of them,10 were normal full-term pregnancy women,15 were complicated with mild ICP and another 15,with severe ICP.Western blot was performed determine the protein level of placental HIF-2α.Results:HIF-2αlevel was different among the three groups(P <0.05),and higher in mild and severe ICP group as compared with nor-mal controls (P <0.05),yet difference was not significant between mild and severe ICP group(P <0.05).Conclusion:HIF-2αprotein is highly ex-pressed in placenta in ICP pregnancy,suggesting that it play an important role in regulating the compensatory homeostatic response to hypoxemia of placen-ta-fetus system.